目的观察开心散治疗乳腺癌失眠症患者的临床疗效及对血清5-羟色胺(5-hydroxytryptamine,5-HT)水平的影响。方法将63例乳腺癌失眠症患者随机分为对照组33例和治疗组30例。对照组患者采用艾司唑仑治疗,治疗组患者采用开心散联合艾司唑仑...目的观察开心散治疗乳腺癌失眠症患者的临床疗效及对血清5-羟色胺(5-hydroxytryptamine,5-HT)水平的影响。方法将63例乳腺癌失眠症患者随机分为对照组33例和治疗组30例。对照组患者采用艾司唑仑治疗,治疗组患者采用开心散联合艾司唑仑治疗。比较两组治疗前后匹兹堡睡眠质量指数量表(Pittsburgh sleep quality index,PSQI)、抑郁自评量表(self-rating depression scale,SDS)、焦虑自评量表(self-rating anxiety scale,SAS)、欧洲癌症研究和治疗协作组编制的癌症患者生活质量评分(European organization for research and treatment of cancer quality of life questionnaire-core 30,EORTC QLQ-C30)以及血清5-HT水平;在连续治疗4周后,比较两组临床疗效及不良反应发生率。结果治疗4周后,两组PSQI、SDS、SAS评分均较治疗前显著降低(P<0.05),且治疗组PSQI评分显著低于对照组(P<0.05);治疗组EORTC QLQ-C30躯体功能、情绪功能、认知功能以及社会功能评分均显著高于对照组(P<0.05);两组患者治疗后血清5-HT水平较治疗前显著升高(P<0.05),且治疗组血清5-HT水平高于对照组(P<0.05);治疗组疗效优于对照组(P<0.05);治疗组总不良反应发生率低于对照组,差异有统计学意义(P<0.05)。结论相较于单纯艾司唑仑治疗,开心散联合艾司唑仑能更好地改善乳腺癌患者睡眠,升高血清5-HT水平,提高乳腺癌患者生活质量,降低不良反应发生率。展开更多
OBJECTIVE:To verify the hypothesis that electroacupuncture inhibits the hyperactivity of the hypothalamic-pituitary-adrenal(HPA)axis via regulating the expression of glial fibrillary acidic protein(GFAP)in the hippoca...OBJECTIVE:To verify the hypothesis that electroacupuncture inhibits the hyperactivity of the hypothalamic-pituitary-adrenal(HPA)axis via regulating the expression of glial fibrillary acidic protein(GFAP)in the hippocampus of acute myocardial ischemia(AMI)rats.METHODS:Sixty-six healthy male Sprague-Dawley rats were randomly divided into five groups:Sham,AMI(Model),electroacupuncture at Shenmen(HT7)-Tongli(HT5)segment(EA),non-acupoint electroacupuncture(Control),and Model+corticosterone(Model+CORT).AMI was induced via occlusion of the left anterior descending coronary artery,followed by 3 d of electroacupuncture at Shenmen(HT7)-Tongli(HT5)segment.In the Control group,electroacupuncture was applied at points lying 5 and 10 mm from the base of the tail.The AMI+CORT group was injected with CORT(20 mg/kg)in saline.Hemorheology,electrocardiography(ECG),hematoxylin and eosin staining,and expression of glycogen phosphorylase BB(GPBB)and heart-type fatty acid-binding protein(H-FABP)were used to assess cardiac function.The effects of adrenocorticotropic hormone(ACTH)and CORT were evaluated by enzymelinked immunosorbent assay.Protein expression in the Sham and Model groups were screened by tandem mass tag-based quantitative proteomics analysis.Protein expression was evaluated by Western blotting(vimentin and GFAP)and immunofluorescence staining(GFAP).RESULTS:Compared with the Sham group,the hemorheology indicators,heart rate,ECG-ST segment elevation,and GPBB and H-FABP levels were higher in Model rats.The EA group showed reductions in these indicators compared with the Model group.Similarly,in Model rats,the expression of ACTH and CORT were significantly increased compared with the Sham group.The EA group also showed reduced expression of ACTH and CORT.Importantly,proteomics analysis showed that vimentin was differentially expressed in Model rats.Compared with the Sham group,vimentin and GFAP expression in the hippocampus was increased in the Model group but decreased in the AMI+EA group.Additionally,intraperitoneal injection of CORT aggravated the expression of GPBB,H-FABP and GFAP.CONCLUSIONS:Our results suggested that electroacupuncture may protect against cardiac injury induced by AMI through regulation of HPA axis hyperactivity,and that hippocampal GFAP may play an important role in the regulation.展开更多
文摘目的观察开心散治疗乳腺癌失眠症患者的临床疗效及对血清5-羟色胺(5-hydroxytryptamine,5-HT)水平的影响。方法将63例乳腺癌失眠症患者随机分为对照组33例和治疗组30例。对照组患者采用艾司唑仑治疗,治疗组患者采用开心散联合艾司唑仑治疗。比较两组治疗前后匹兹堡睡眠质量指数量表(Pittsburgh sleep quality index,PSQI)、抑郁自评量表(self-rating depression scale,SDS)、焦虑自评量表(self-rating anxiety scale,SAS)、欧洲癌症研究和治疗协作组编制的癌症患者生活质量评分(European organization for research and treatment of cancer quality of life questionnaire-core 30,EORTC QLQ-C30)以及血清5-HT水平;在连续治疗4周后,比较两组临床疗效及不良反应发生率。结果治疗4周后,两组PSQI、SDS、SAS评分均较治疗前显著降低(P<0.05),且治疗组PSQI评分显著低于对照组(P<0.05);治疗组EORTC QLQ-C30躯体功能、情绪功能、认知功能以及社会功能评分均显著高于对照组(P<0.05);两组患者治疗后血清5-HT水平较治疗前显著升高(P<0.05),且治疗组血清5-HT水平高于对照组(P<0.05);治疗组疗效优于对照组(P<0.05);治疗组总不良反应发生率低于对照组,差异有统计学意义(P<0.05)。结论相较于单纯艾司唑仑治疗,开心散联合艾司唑仑能更好地改善乳腺癌患者睡眠,升高血清5-HT水平,提高乳腺癌患者生活质量,降低不良反应发生率。
文摘目的探析人参Panax ginseng近年来的研究热点与前沿,为人参的研究进展做全面概述,帮助迅速把握其研究内涵和研究方向并为今后的研究工作提供见解。方法通过检索中国知识资源总库(CNKI)、万方数据库、维普数据库、中国生物医学文献数据库和Web of Science核心合集数据库2003年1月1日-2023年12月31日收录的中英文人参相关文献,采用CiteSpace和VOSviewer软件分别对已发表的中英文文献作者、机构和关键词绘制成图谱,并进行共现、聚类和时间线可视化分析。结果共纳入文献18833篇,其中中文文献10373篇,英文文献8460篇。近20年人参的中、英文发文量均呈现阶段性变化趋势,整体上呈现稳定增长态态势。中、英文文献发文量最多的作者分别是张连学和Yang Deok-Chun;发文量最多的研究机构分别为中国吉林农业大学和韩国庆熙大学;中国是英文文献发文量最多的国家,且与其他国家保持广泛的联系。结论人参研究当前主要集中在其化学成分分析、临床应用、药理作用、药物栽培等方面,并已得到国内外学者的关注,具有良好的发展势头。人参抗衰老、抗肿瘤机制与糖尿病的防治是现阶段研究热点,预计在未来一段时间内将持续受到关注。同时,中医药理论指导下人参功效的现代科学解释仍需进一步深入研究。
基金Natural Science Foundation-funded Project:Exploring the Mechanism of Anti-Ischemic Effect of Acupuncture Based on the Regulation of Hippocampal-HPA Axis by Glutamatergic Neurons(No.82004462)Opening Project of Zhejiang Provincial Preponderant and Characteristic Subject of Key University(Chinese Traditional Medicine):Study of the Mechanism of Action of Acupuncture in the Treatment of Myocardial Ischemia in Rats Based on the Amygdala Glutamatergic System-Mediated Affective Changes(ZYXYB2019002)+1 种基金National Key Research and Development Program of China:Influence of Heart and Lung Meridians on Heart and Lung Function(No.SQ2018YFC170298)National Natural Science Foundation of Anhui:Mechanism of Action of Intestinal Flora Involved in the Intervention of Myocardial Ischemia by Acupuncture of the Heart Meridian(No.1908085MH289)。
文摘OBJECTIVE:To verify the hypothesis that electroacupuncture inhibits the hyperactivity of the hypothalamic-pituitary-adrenal(HPA)axis via regulating the expression of glial fibrillary acidic protein(GFAP)in the hippocampus of acute myocardial ischemia(AMI)rats.METHODS:Sixty-six healthy male Sprague-Dawley rats were randomly divided into five groups:Sham,AMI(Model),electroacupuncture at Shenmen(HT7)-Tongli(HT5)segment(EA),non-acupoint electroacupuncture(Control),and Model+corticosterone(Model+CORT).AMI was induced via occlusion of the left anterior descending coronary artery,followed by 3 d of electroacupuncture at Shenmen(HT7)-Tongli(HT5)segment.In the Control group,electroacupuncture was applied at points lying 5 and 10 mm from the base of the tail.The AMI+CORT group was injected with CORT(20 mg/kg)in saline.Hemorheology,electrocardiography(ECG),hematoxylin and eosin staining,and expression of glycogen phosphorylase BB(GPBB)and heart-type fatty acid-binding protein(H-FABP)were used to assess cardiac function.The effects of adrenocorticotropic hormone(ACTH)and CORT were evaluated by enzymelinked immunosorbent assay.Protein expression in the Sham and Model groups were screened by tandem mass tag-based quantitative proteomics analysis.Protein expression was evaluated by Western blotting(vimentin and GFAP)and immunofluorescence staining(GFAP).RESULTS:Compared with the Sham group,the hemorheology indicators,heart rate,ECG-ST segment elevation,and GPBB and H-FABP levels were higher in Model rats.The EA group showed reductions in these indicators compared with the Model group.Similarly,in Model rats,the expression of ACTH and CORT were significantly increased compared with the Sham group.The EA group also showed reduced expression of ACTH and CORT.Importantly,proteomics analysis showed that vimentin was differentially expressed in Model rats.Compared with the Sham group,vimentin and GFAP expression in the hippocampus was increased in the Model group but decreased in the AMI+EA group.Additionally,intraperitoneal injection of CORT aggravated the expression of GPBB,H-FABP and GFAP.CONCLUSIONS:Our results suggested that electroacupuncture may protect against cardiac injury induced by AMI through regulation of HPA axis hyperactivity,and that hippocampal GFAP may play an important role in the regulation.
