The Central Asian Orogenic Belt(CAOB)is a huge tectonic mélange that lies between the North China Craton and the Siberian Block.It is composed of multiple orogenic belts,continental fragments,magmatic and metamor...The Central Asian Orogenic Belt(CAOB)is a huge tectonic mélange that lies between the North China Craton and the Siberian Block.It is composed of multiple orogenic belts,continental fragments,magmatic and metamorphic rocks,suture zones and discontinuous ophiolite belts.Although the Hegenshan and Sartohay ophiolites are separated by nearly 3000 km and lie in completely different parts of the CAOB,they are remarkably similar in many respects.Both are composed mainly of serpentinized peridotite and dunite,with minor gabbro and sparse basalt.They both host significant podiform chromitites that consist of high-Al,refractory magnesiochromite with Cr#s[100Cr/(Cr+Al)]averaging<60.The Sartohay ophiolite has a zircon U-Pb age of ca.300 Ma and has been intruded by granitic plutons of similar age,resulting in intense hydrothermal activity and the formation of gold-bearing listwanites.The age of the Hegenshan is not firmly established but is thought to have formed in the Carboniferous.Like many other ophiolites that we have investigated in other orogenic belts,the chromitites in these two bodieshave abundant diamonds,as well as numerous super-reduced and crustal minerals.The diamonds are mostly,colorless to pale yellow,200-300μm across and have euhedral to anhedral shapes.They all have low carbon isotopes(δ14C=-18 to-29)and some have visible inclusions.These are accompanied by numerous super-reduced minerals such as moissanite,native elements(Fe,Cr,Si,Al,Mn),and alloys(e.g.,Ni-Mn-Fe,Ni-Fe-Al,Ni-Mn-Co,Cr-Ni-Fe,Cr-Fe,Cr-Fe-Mn),as well as a wide range of oxides,sulfides and silicates.Grains of zircon are abundant in the chromitites of both ophiolites and range in age from Precambrian to Cretaceous,reflecting both incorporation of old zircons and modification of grains by hydrothermal alteration.Our investigation confirms that high-Al,refractory chromitites in these two ophiolites have the same range of exotic minerals as high-Cr metallurgical chromitites such as those in the Luobusa ophiolite of Tibet.These collections of exotic minerals in ophiolitic chromitites indicate complex,multi-stage recycling of oceanic and continental crustal material at least to the mantle transition zone,followed by uprise and emplacement of the peridotites into relatively shallow ophiolites.展开更多
This study aims at evaluating the heavy metals pollution in soil collected from Penglai Fairyland Park in Guiyang,and predicting the harm of heavy metals to human health. Based on the background value of soil in Guizh...This study aims at evaluating the heavy metals pollution in soil collected from Penglai Fairyland Park in Guiyang,and predicting the harm of heavy metals to human health. Based on the background value of soil in Guizhou and Soil Environment Quality Standard( GB15618-1995),by using the geoaccumulation index method and Hakanson potential ecological risk assessment method,we research pollution characteristics and ecological risk of heavy metals in soil from the study area. The results show that from the index of geoaccumulation,the pollution level of heavy metals in agricultural soil from Penglai Fairyland Park is in the order of Ni > Cu > Hg > Cd > As > Zn > Cr > Pb. From the potential ecological harm index evaluation,soil is at slight ecological risk level in the park and the potential ecological hazard degree is in the order of Hg > Cd > Ni > As > Cu > Cr > Pb > Zn. The soil ecological environment in research area is only mildly damaged. Thus,the soil in research area is at the level of security,and it is suitable for cultivation of crops and less harmful to human health.展开更多
Predicting the external flow field with limited data or limited measurements has attracted long-time interests of researchers in many industrial applications.Physics informed neural network(PINN)provides a seamless fr...Predicting the external flow field with limited data or limited measurements has attracted long-time interests of researchers in many industrial applications.Physics informed neural network(PINN)provides a seamless framework for combining the measured data with the deep neural network,making the neural network capable of executing certain physical constraints.Unlike the data-driven model to learn the end-to-end mapping between the sensor data and high-dimensional flow field,PINN need no prior high-dimensional field as the training dataset and can construct the mapping from sensor data to high dimensional flow field directly.However,the extrapolation of the flow field in the temporal direction is limited due to the lack of training data.Therefore,we apply the long short-term memory(LSTM)network and physics-informed neural network(PINN)to predict the flow field and hydrodynamic force in the future temporal domain with limited data measured in the spatial domain.The physical constraints(conservation laws of fluid flow,e.g.,Navier-Stokes equations)are embedded into the loss function to enforce the trained neural network to capture some latent physical relation between the output fluid parameters and input tempo-spatial parameters.The sparsely measured points in this work are obtained from computational fluid dynamics(CFD)solver based on the local radial basis function(RBF)method.Different numbers of spatial measured points(4–35)downstream the cylinder are trained with/without the prior knowledge of Reynolds number to validate the availability and accuracy of the proposed approach.More practical applications of flow field prediction can compute the drag and lift force along with the cylinder,while different geometry shapes are taken into account.By comparing the flow field reconstruction and force prediction with CFD results,the proposed approach produces a comparable level of accuracy while significantly fewer data in the spatial domain is needed.The numerical results demonstrate that the proposed approach with a specific deep neural network configuration is of great potential for emerging cases where the measured data are often limited.展开更多
OBJECTIVE: To evaluate the protective effect of puerarin [an isoflavone compound extracted from Gegen(Radix Puerariae Lobatae)] in scleral remodeling induced by extremely low frequency electromagnetic fields(ELF-EMFs)...OBJECTIVE: To evaluate the protective effect of puerarin [an isoflavone compound extracted from Gegen(Radix Puerariae Lobatae)] in scleral remodeling induced by extremely low frequency electromagnetic fields(ELF-EMFs).METHODS: Human fetal scleral fibroblasts(HFSFs)were divided into 5 groups:(a) untreated controls;(b) cells treated with ELF-EMFs;(c) cells treated with ELF-EMFs and puerarin 0.1 μM;(d) cells treated with ELF-EMFs and puerarin 1 μM;(e) cells treated with ELF-EMFs and puerarin 10 μM. Cell proliferation activity was measured by the cell-counting kit-8 assay. Matrix metalloproteinase-2(MMP-2) activity was measured by gelatin enzymography.MMP-2 and collagenⅠ(COL1A1) m RNA, protein expression were measured by Real-Time polymerase chain reaction, Western blot analysis, respectively.RESULTS: Puerarin reduced the inhibition in cell proliferation, MMP-2 activity, m RNA, protein expression of HFSFs exposed to ELF-EMFs and enhanced the COL1A1 m RNA and protein expression.CONCLUSION: Puerarin was found to participate in the matrix remodeling process. It might be a potential agent for the treatment of extracellular matrix degradation of sclera associated with ocular conditions.展开更多
The balanced actions between ubiquitination and deubiquitination precisely control the levels of various proteins vital for spermatogenesis. Ubiquitin-specific processing proteases(USPs) are the largest family of deub...The balanced actions between ubiquitination and deubiquitination precisely control the levels of various proteins vital for spermatogenesis. Ubiquitin-specific processing proteases(USPs) are the largest family of deubiquitinatingenzymes(DUBs),containing more than 50 members. So far, the functions of only a few USPs in male fertility have been studied, the roles of the majority are yet unknown. The present study aimed to explore the function of Usp29(ubiquitin-specific protease 29) in male fertility. We found that Usp29 showed predominant expression in mouse testis, and its m RNA expression started to increase at 14 days postpartum(dpp), with a peak at 28 and 35 dpp. Using CRISPR/Cas9 technology, we generated Usp29 knockout mice(Usp29^(–/–)). Usp29^(–/–)mice exhibited no overt developmental anomalies. Further examination revealed that Usp29^(–/–)mice had normal fertility and showed no detectable difference in the testis/body weight ratio, testicular and epididymal histology as well as epididymal sperm count from the wild-type littermates. Moreover, Usp29 is not a pseudogene in mice. Taken together, our study first reported that though Usp29 is predominantly expressed in the testis, it is not essential for male fertility in mice.展开更多
Background The thirteen-valent pneumococcal conjugate vaccine(PCV13)is not included in the national immu-nization program and is administered voluntarily with informed consent in China.In preparation for assessing the...Background The thirteen-valent pneumococcal conjugate vaccine(PCV13)is not included in the national immu-nization program and is administered voluntarily with informed consent in China.In preparation for assessing the impact of pilot introduction in Hainan Province,we conducted a carriage study among children under 5 years of age from four locations in Hainan Province,China.Methods From March to June 2022,nasopharyngeal(NP)swabs,collected from healthy children aged younger than 59 months who lived in the 4 different locations(Haikou,Wanning,Baisha and Qiongzhong)in Hainan Province,were tested for pneumococcus using conventional culture.Pneumococcal isolates were serotyped using the Quel-lung reaction.Risk factors associated with pneumococcal colonization were assessed using univariate analysis and multivariable logistic regression adjusting for age,daycare attendance and other factors.Results Pneumococcus was isolated in 710(30.4%)of the 2333 children enrolled.Of 737 pneumococci,29 serotypes were identifed;60.9%were PCV13 serotypes;the most common vaccine serotypes were 6B(20.4%),19F(13.0%),6A(11.9%)and 23F(6.1%);and the most common nonvaccine serotypes were 23A(12.9%),34(6.1%)and nontypeable(NT)pneumococci(5.6%).Children vaccinated with PCV13 had lower carriage(17.7%vs 32.5%;P=0.0001)and fewer PCV13 serotypes(41.9%vs 62.7%;P=0.0017)compared to unimmunized children.After adjustment,NP carriage Was higher among children attending daycare(aOR=2.3,95%CI:1.7-3.2),living in rural areas(aOR=1.4,95%CI:1.1-1.8),living with siblings(aOR=1.3,95%CI:1.0-1.6)and whose mothers had completed senior high/technical sec-ondary school(aOR=1.5,95%CI:1.1-2.0).In contrast,completion of 3-4 doses of PCV13 were associated with a lower carriage rate(aOR=0.6,95%CI:0.4-0.9).Conclusions We established the baseline of pneumococcal carriage,serotype distribution and PCV13 immuni-zation rates among healthy children under 5 years of age in Hainan Province,prior to the introduction of PCV13 into the national immunization program.The high proportion of PCV13 serotypes suggests that PCV13 introduction will likely have a substantial impact on pneumococcal carriage in Hainan Province.展开更多
文摘The Central Asian Orogenic Belt(CAOB)is a huge tectonic mélange that lies between the North China Craton and the Siberian Block.It is composed of multiple orogenic belts,continental fragments,magmatic and metamorphic rocks,suture zones and discontinuous ophiolite belts.Although the Hegenshan and Sartohay ophiolites are separated by nearly 3000 km and lie in completely different parts of the CAOB,they are remarkably similar in many respects.Both are composed mainly of serpentinized peridotite and dunite,with minor gabbro and sparse basalt.They both host significant podiform chromitites that consist of high-Al,refractory magnesiochromite with Cr#s[100Cr/(Cr+Al)]averaging<60.The Sartohay ophiolite has a zircon U-Pb age of ca.300 Ma and has been intruded by granitic plutons of similar age,resulting in intense hydrothermal activity and the formation of gold-bearing listwanites.The age of the Hegenshan is not firmly established but is thought to have formed in the Carboniferous.Like many other ophiolites that we have investigated in other orogenic belts,the chromitites in these two bodieshave abundant diamonds,as well as numerous super-reduced and crustal minerals.The diamonds are mostly,colorless to pale yellow,200-300μm across and have euhedral to anhedral shapes.They all have low carbon isotopes(δ14C=-18 to-29)and some have visible inclusions.These are accompanied by numerous super-reduced minerals such as moissanite,native elements(Fe,Cr,Si,Al,Mn),and alloys(e.g.,Ni-Mn-Fe,Ni-Fe-Al,Ni-Mn-Co,Cr-Ni-Fe,Cr-Fe,Cr-Fe-Mn),as well as a wide range of oxides,sulfides and silicates.Grains of zircon are abundant in the chromitites of both ophiolites and range in age from Precambrian to Cretaceous,reflecting both incorporation of old zircons and modification of grains by hydrothermal alteration.Our investigation confirms that high-Al,refractory chromitites in these two ophiolites have the same range of exotic minerals as high-Cr metallurgical chromitites such as those in the Luobusa ophiolite of Tibet.These collections of exotic minerals in ophiolitic chromitites indicate complex,multi-stage recycling of oceanic and continental crustal material at least to the mantle transition zone,followed by uprise and emplacement of the peridotites into relatively shallow ophiolites.
基金Supported by Agricultural Science and Technology Achievement Transformation Fund Project of the Ministry of Science and Technology(2013F-2001007)Project of Edible Fungus Engineering Center of Guizhou Province(G20144002)+1 种基金Guiyang Science and Technology Project(201410014)Project of Baiyun District Science and Technology Bureau(201419)
文摘This study aims at evaluating the heavy metals pollution in soil collected from Penglai Fairyland Park in Guiyang,and predicting the harm of heavy metals to human health. Based on the background value of soil in Guizhou and Soil Environment Quality Standard( GB15618-1995),by using the geoaccumulation index method and Hakanson potential ecological risk assessment method,we research pollution characteristics and ecological risk of heavy metals in soil from the study area. The results show that from the index of geoaccumulation,the pollution level of heavy metals in agricultural soil from Penglai Fairyland Park is in the order of Ni > Cu > Hg > Cd > As > Zn > Cr > Pb. From the potential ecological harm index evaluation,soil is at slight ecological risk level in the park and the potential ecological hazard degree is in the order of Hg > Cd > Ni > As > Cu > Cr > Pb > Zn. The soil ecological environment in research area is only mildly damaged. Thus,the soil in research area is at the level of security,and it is suitable for cultivation of crops and less harmful to human health.
基金supported by the National Natural Science Foundation of China(Grant Nos.52206053,52130603)。
文摘Predicting the external flow field with limited data or limited measurements has attracted long-time interests of researchers in many industrial applications.Physics informed neural network(PINN)provides a seamless framework for combining the measured data with the deep neural network,making the neural network capable of executing certain physical constraints.Unlike the data-driven model to learn the end-to-end mapping between the sensor data and high-dimensional flow field,PINN need no prior high-dimensional field as the training dataset and can construct the mapping from sensor data to high dimensional flow field directly.However,the extrapolation of the flow field in the temporal direction is limited due to the lack of training data.Therefore,we apply the long short-term memory(LSTM)network and physics-informed neural network(PINN)to predict the flow field and hydrodynamic force in the future temporal domain with limited data measured in the spatial domain.The physical constraints(conservation laws of fluid flow,e.g.,Navier-Stokes equations)are embedded into the loss function to enforce the trained neural network to capture some latent physical relation between the output fluid parameters and input tempo-spatial parameters.The sparsely measured points in this work are obtained from computational fluid dynamics(CFD)solver based on the local radial basis function(RBF)method.Different numbers of spatial measured points(4–35)downstream the cylinder are trained with/without the prior knowledge of Reynolds number to validate the availability and accuracy of the proposed approach.More practical applications of flow field prediction can compute the drag and lift force along with the cylinder,while different geometry shapes are taken into account.By comparing the flow field reconstruction and force prediction with CFD results,the proposed approach produces a comparable level of accuracy while significantly fewer data in the spatial domain is needed.The numerical results demonstrate that the proposed approach with a specific deep neural network configuration is of great potential for emerging cases where the measured data are often limited.
基金Supported by the General Program of the Bio-medical Division of the Shanghai Science and Technology Commission:Effect of Extremely Low Frequency Electromagnetic Fields on Myopia Based on RPE-sclera Complex(No.10411966200)the Scientific Research Fund of Chinese Medical of Shanghai Health Bureau:Effect of Puerarin on RPE Cells Exposed to ELF-EMF(No.2014JP015)
文摘OBJECTIVE: To evaluate the protective effect of puerarin [an isoflavone compound extracted from Gegen(Radix Puerariae Lobatae)] in scleral remodeling induced by extremely low frequency electromagnetic fields(ELF-EMFs).METHODS: Human fetal scleral fibroblasts(HFSFs)were divided into 5 groups:(a) untreated controls;(b) cells treated with ELF-EMFs;(c) cells treated with ELF-EMFs and puerarin 0.1 μM;(d) cells treated with ELF-EMFs and puerarin 1 μM;(e) cells treated with ELF-EMFs and puerarin 10 μM. Cell proliferation activity was measured by the cell-counting kit-8 assay. Matrix metalloproteinase-2(MMP-2) activity was measured by gelatin enzymography.MMP-2 and collagenⅠ(COL1A1) m RNA, protein expression were measured by Real-Time polymerase chain reaction, Western blot analysis, respectively.RESULTS: Puerarin reduced the inhibition in cell proliferation, MMP-2 activity, m RNA, protein expression of HFSFs exposed to ELF-EMFs and enhanced the COL1A1 m RNA and protein expression.CONCLUSION: Puerarin was found to participate in the matrix remodeling process. It might be a potential agent for the treatment of extracellular matrix degradation of sclera associated with ocular conditions.
基金Project supported by the Natural Science Foundation of Zhejiang Province(No.LY14H120003)the Qianjiang Talent Project of Zhejiang Province(No.2013R10040),China
基金supported by the National Key Research and Developmental Program of China(2018YFC1004700 and 2016YFC1000600)the Strategic Priority Research Program of the Chinese Academy of Sciences(XDB19000000)+1 种基金the National Natural Science Foundation of China(31401953,31630050,31890780,31871514 and 81571495)Major Program of Development Foundation of Hefei Centre for Physical Science and Technology(2018ZYFX005)
文摘The balanced actions between ubiquitination and deubiquitination precisely control the levels of various proteins vital for spermatogenesis. Ubiquitin-specific processing proteases(USPs) are the largest family of deubiquitinatingenzymes(DUBs),containing more than 50 members. So far, the functions of only a few USPs in male fertility have been studied, the roles of the majority are yet unknown. The present study aimed to explore the function of Usp29(ubiquitin-specific protease 29) in male fertility. We found that Usp29 showed predominant expression in mouse testis, and its m RNA expression started to increase at 14 days postpartum(dpp), with a peak at 28 and 35 dpp. Using CRISPR/Cas9 technology, we generated Usp29 knockout mice(Usp29^(–/–)). Usp29^(–/–)mice exhibited no overt developmental anomalies. Further examination revealed that Usp29^(–/–)mice had normal fertility and showed no detectable difference in the testis/body weight ratio, testicular and epididymal histology as well as epididymal sperm count from the wild-type littermates. Moreover, Usp29 is not a pseudogene in mice. Taken together, our study first reported that though Usp29 is predominantly expressed in the testis, it is not essential for male fertility in mice.
基金This research was funded by Bill&Melinda Gates Foundation(No.INV-034554)Beijing Municipal Natural Science Foundation(No.L202007)Special funds for the construction of high-level public health technical personnel from Beijing Municipal Health Commission(No.2022-3-01-021).
文摘Background The thirteen-valent pneumococcal conjugate vaccine(PCV13)is not included in the national immu-nization program and is administered voluntarily with informed consent in China.In preparation for assessing the impact of pilot introduction in Hainan Province,we conducted a carriage study among children under 5 years of age from four locations in Hainan Province,China.Methods From March to June 2022,nasopharyngeal(NP)swabs,collected from healthy children aged younger than 59 months who lived in the 4 different locations(Haikou,Wanning,Baisha and Qiongzhong)in Hainan Province,were tested for pneumococcus using conventional culture.Pneumococcal isolates were serotyped using the Quel-lung reaction.Risk factors associated with pneumococcal colonization were assessed using univariate analysis and multivariable logistic regression adjusting for age,daycare attendance and other factors.Results Pneumococcus was isolated in 710(30.4%)of the 2333 children enrolled.Of 737 pneumococci,29 serotypes were identifed;60.9%were PCV13 serotypes;the most common vaccine serotypes were 6B(20.4%),19F(13.0%),6A(11.9%)and 23F(6.1%);and the most common nonvaccine serotypes were 23A(12.9%),34(6.1%)and nontypeable(NT)pneumococci(5.6%).Children vaccinated with PCV13 had lower carriage(17.7%vs 32.5%;P=0.0001)and fewer PCV13 serotypes(41.9%vs 62.7%;P=0.0017)compared to unimmunized children.After adjustment,NP carriage Was higher among children attending daycare(aOR=2.3,95%CI:1.7-3.2),living in rural areas(aOR=1.4,95%CI:1.1-1.8),living with siblings(aOR=1.3,95%CI:1.0-1.6)and whose mothers had completed senior high/technical sec-ondary school(aOR=1.5,95%CI:1.1-2.0).In contrast,completion of 3-4 doses of PCV13 were associated with a lower carriage rate(aOR=0.6,95%CI:0.4-0.9).Conclusions We established the baseline of pneumococcal carriage,serotype distribution and PCV13 immuni-zation rates among healthy children under 5 years of age in Hainan Province,prior to the introduction of PCV13 into the national immunization program.The high proportion of PCV13 serotypes suggests that PCV13 introduction will likely have a substantial impact on pneumococcal carriage in Hainan Province.