多普勒超声联合血清RBP及HCY检测对早期卵巢癌的预测价值

目的探究并分析多普勒超声联合血清RBP(视黄醇结合蛋白)及HCY(同型半胱氨酸)检测对早期卵巢癌的预测价值。方法选取2015年7月-2016年12月收治的50例早期卵巢癌患者,50例卵巢良性肿瘤患者及50名正常志愿者,分别作为早期卵巢癌组、卵巢良性肿瘤组及对照组。测定各组患者血清RBP、HCY浓度,对所有受试者进行超声检查,记录下患者卵巢超声评分。采用血清RBP、HCY、超声评分、超声评分+RBP+Hcy作为诊断指标对所有受试者进行诊断,比较上述诊断方法的准确性(包括敏感性、特异性及一致性),分析超声评分与卵巢癌病变的相关性。结果肿瘤性质、单侧病变最大直径、单双侧、腹腔积液、肿瘤分期与卵巢癌病变具有一定的相关性(P <0.05);肿瘤性质、单侧病变最大直径、肿瘤分期与卵巢恶性病变具有显著相关性(P <0.05,OR=1.385、1.547、1.521);早期卵巢癌组患者RBP浓度明显低于卵巢良性肿瘤组及对照组(P <0.05),卵巢良性肿瘤组R BP浓度明显低于对照组(P <0.05),早期卵巢癌组患者Hcy浓度、超声评分明显高于卵巢良性肿瘤组及对照组(P <0.05),卵巢良性肿瘤组Hcy浓度、超声评分明显高于对照组(P <0.05),具有统计学意义。超声评分+RBP+Hcy联合诊断敏感度明显高于单纯RBP、Hcy、超声评分(P <0.05),超声评分+RBP+Hcy联合诊断特异性低于单纯RBP、Hcy、超声评分诊断,超声评分+RBP+Hcy联合诊断一致性高于单纯RBP、Hcy、超声评分诊断。结论超声评分+RBP+Hcy联合诊断较单纯应用RBP、Hcy、超声评分更能有效增加卵巢癌准确率,值得临床推广。

N-hexane Alters the Maturation of Oocytes and Induces Apoptosis in Mice

Objective This study was aimed to determine the effects of n-hexane on the maturation of mouse oocytes. Methods Cell culture was used to observe the maturation of mouse oocytes and CLSM was employed to determine their apoptosis. Results Germinal vesicle breakdown （GVBD） and extrusion of the first polar body in mouse oocytes were significantly inhibited by n-hexane. After fertilization, the number of eggs in the mouse was significantly reduced by n-hexane. Mitochondrial membrane potentials （A^Um） were altered in mouse oocytes that were leading to apoptosis of the oocytes. Conclusion N-hexane might have affected the maturation of oocytes, causing alteration of ~qJm and leading to apoptosis which maybe one of the most imn（~rt^nt rnpch^ni^nn~