基于生物信息学分析SPHK1在胃癌中的表达及临床价值

目的:研究SPHK1在胃癌组织中的表达及利用生物信息学分析其在胃癌中的临床意义。方法:利用HPA和TCGA数据库分析SPHK1在胃癌组织中的表达。采用GEPIA数据库分析SPHK1与MKI67两者在胃癌中表达的关联。基于Kaplan-Meier Plotter数据库分析SPHK1 mRNA表达水平与胃癌患者生存期的关系。基因富集通路预测SPHK1对胃癌潜在的生物学功能。采用TIMER数据库,预测SPHK1在免疫浸润细胞中的表达。通过IHC和Western Blot分析SPHK1在胃癌组织中的表达。结果:生物信息学分析表明SPHK1在胃癌组织中较癌旁高表达(P<0.001)。GEPIA数据库分析显示胃癌组织中SPHK1和MKI67表达较高(P<0.05),且二者呈正相关(P=0.000 49)。Kaplan-Meier Plotter生存分析表明,高表达SPHK1在胃癌中预示更差的总生存期(log rank P=0.002)和进展后生存期(log rank P=0.001 9)。基因富集通路数据显示,SPHK1可能参与多个胃癌进展通路。TIMER数据库分析表明,在巨噬细胞中,低SPHK1表达组胃癌患者的进展后生存期优于SPHK1高表达组(P=0.002)。IHC显示,SPHK1在胃癌组织中的染色强度和阳性面积均高于邻近非肿瘤组织,且染色强度TNMI、Ⅱ期弱于Ⅲ、Ⅳ期。Western Blot显示,在9例胃癌组织及配对癌旁组织中,SPHK1蛋白水平在胃癌组织中表达更高(P=0.026 5)。结论:SPHK1基因在胃癌组织中高表达,并与不良预后相关,且可能降低肿瘤免疫抑制,因而可作为评估胃癌患者预后的潜在标志物。

SPHK1 expression in gastric cancer and clinical value based on bioinformatics analysis

Objective:To elucidate the expression of SPHK1 in gastric cancer tissues and to analyse its clinical value in gastric cancer using bioinformatics.Methods:The expression of SPHK1 in gastric cancer tissues was analysed using HPA and TCGA databases.The association between the expression of both SPHK1 and MKI67 in gastric cancer was analysed using GEPIA database.Based on Kaplan-Meier Plotter database to analyse the relationship between SPHK1 mRNA expression level and survival of gastric cancer patients.Gene enrichment pathway predicted the potential biological function of SPHK1 on gastric cancer.The TIMER database was used to predict the expression of SPHK1 in immune-infiltrating cells.The expression of SPHK1 in gastric cancer tissues was analysed by IHC and Western Blot.Results:Bioinformatics analysis showed that SPHK1 was highly expressed in gastric cancer tissues compared to paracancerous tissues(P<0.001).The analysis from the GEPIA database reveals a significant increase in the expression of SPHK1 and MKI67 in gastric cancer tissues(P<0.05).Furthermore,there is a positive correlation between the two proteins(P=0.00049).Kaplan-Meier Plotter survival analysis showed that high expression of SPHK1 predicted worse overall survival tine(logrankP=0.002)and post-progression generation time(logrankP=0.0019)in gastric cancer.Gene enrichment pathway results showed that SPHK1 may be involved in multiple gastric cancer progression pathways.TIMER database analysis showed that in macrophages,gastric cancer patients with low SPHK1 expression group had a better post-progression period than those with high SPHK1 expression group(P=0.002).IHC showed that the staining intensity and staining positive area of SPHK1 in gastric cancer tissues were higher than that of adjacent non-tumour tissues.And the staining intensity of TNMI and Ⅱ stages was weaker than that of Ⅲ and Ⅳ stages.Western blot analysis revealed higher levels of SPHK1 protein in gastric cancer tissue compared to paired adjacent non-cancerous tissue in 9 cases(P=0.0265).Conclusion:SPHK1 gene is highly expressed in gastric cancer tissues and is associated with poor prognosis and may reduce tumour immunosuppression,which may serve as a potential marker for evaluating the prognosis of gastric cancer patients.

安徽省大学生艾滋病知信行情况及影响因素与宣传方式探究

目的了解安徽省大学生关于艾滋病的知信行情况及影响因素,评估不同宣传方式的干预效果。方法采取分层整群抽样方法抽取安徽省10所高校共计1178名学生,进行艾滋病知信行调查并分析影响因素,再根据答题情况分组分别给予一般健康教育、交互式健康教育,统计分析后比较效果。结果安徽省大学生整体上对艾滋病的了解不够全面,总体得分(70.54±10.91)分,部分问题知晓率低于60%。经Logistic回归分析,影响大学生艾滋病知识知晓率的因素主要是年级(OR=2.455, 95%CI:1.894～3.739, P=0.031)和专业(OR=0.084, 95%CI:0.025～0.283, P<0. 001)。2类宣传方式干预前后大学生的艾滋病相关知识、态度情况差异均有统计学意义(P<0.05),其中交互式健康教育的效果要明显优于一般健康教育(P<0.05)。结论在大学生群体中开展艾滋病宣传教育十分必要,并且应根据教育对象的异质性选取适宜的方式,交互式健康教育的方式值得大力推倡。