为探究急性温度胁迫对红耳龟Trachemys scripta elegans消化和免疫功能的影响及饲料添加维生素E(VE)的干预效应。选取健康2龄红耳龟112只,分成未添加VE组和添加VE组(250 mg·kg^(−1)),每组设室温(30℃)、低温(17℃)和高温(35℃),于...为探究急性温度胁迫对红耳龟Trachemys scripta elegans消化和免疫功能的影响及饲料添加维生素E(VE)的干预效应。选取健康2龄红耳龟112只,分成未添加VE组和添加VE组(250 mg·kg^(−1)),每组设室温(30℃)、低温(17℃)和高温(35℃),于胁迫0 h、6 h、12 h取血液和肠道组织,检测血清抗氧化酶和肠道消化酶活性。结果表明,未添加VE组低温下淀粉酶和脂肪酶的活性显著上升,谷胱甘肽(GSH)含量显著降低,高温下胃蛋白酶活性极显著升高,超氧化物歧化酶(SOD)活性显著升高,GSH含量显著下降;添加VE组急性温度胁迫下的SOD活性升幅减小,GSH含量升高,低温下胃蛋白酶活性显著提高,脂肪酶活性显著降低;高温下胃蛋白酶活性极显著降低,淀粉酶活性显著升高。本研究初步阐释了急性温度胁迫对红耳龟的损伤机制,同时发现饲料添加VE具有一定程度的缓解作用,为生产中应对急性温度应激探索了一条绿色可行途径。展开更多
To confirm phytoplasma infection,samples of pomegranate(Punica granatum L.)plants showing symptoms of fasciation were collected from an orchard located in Tai’an,Shandong Province,China.A fragment of approximately 1....To confirm phytoplasma infection,samples of pomegranate(Punica granatum L.)plants showing symptoms of fasciation were collected from an orchard located in Tai’an,Shandong Province,China.A fragment of approximately 1.2 kb was amplified with universal primers targeting the phytoplasma 16S r RNA gene from symptomatic pomegranate plants,while no fragment was obtained from healthy plants.The phytoplasma associated with the disease was designated as pomegranate fasciation(Po F).Two representative phytoplasma 16S r DNA gene sequences(Po F-Ch01 and Po F-Ch02)had 100%nucleotide sequence identity.The 16S r DNA sequence of Po F-Ch01 and Po F-Ch02 showed the highest similarity(99.6%)to that of‘P.granatum’phytoplasma isolate AY-PG,which belong to 16Sr I-B.Further phylogenetic analysis showed that Po F-Ch01 and Po F-Ch02 belonged to a cluster of 16Sr I subgroup members.In silico RFLP analysis indicated that Po F-Ch01 shared the highest similarity coefficient of 0.97 with reference strains of 16Sr I-B,M and N.Actual RFLP analysis of both enzymes Bst U I and Bfa I confirmed that of the virtual RFLP analysis.Combining these results,we concluded that Po F was a member of the‘Candidatus Phytoplasma asteris’group(16Sr I),and has very close relationship with 16Sr I-B subgroup.展开更多
基金supported by National Natural Science Foundation of China (Grant No. 31401718 )the Foundation for Excellent Young Scientists of Shandong Province (Grant No. BS2013NY012 )+1 种基金Specialized Research Fund for the Doctoral Program of Higher Education (Grant No. SRFDP, 20123702110013 BR0801 )
文摘To confirm phytoplasma infection,samples of pomegranate(Punica granatum L.)plants showing symptoms of fasciation were collected from an orchard located in Tai’an,Shandong Province,China.A fragment of approximately 1.2 kb was amplified with universal primers targeting the phytoplasma 16S r RNA gene from symptomatic pomegranate plants,while no fragment was obtained from healthy plants.The phytoplasma associated with the disease was designated as pomegranate fasciation(Po F).Two representative phytoplasma 16S r DNA gene sequences(Po F-Ch01 and Po F-Ch02)had 100%nucleotide sequence identity.The 16S r DNA sequence of Po F-Ch01 and Po F-Ch02 showed the highest similarity(99.6%)to that of‘P.granatum’phytoplasma isolate AY-PG,which belong to 16Sr I-B.Further phylogenetic analysis showed that Po F-Ch01 and Po F-Ch02 belonged to a cluster of 16Sr I subgroup members.In silico RFLP analysis indicated that Po F-Ch01 shared the highest similarity coefficient of 0.97 with reference strains of 16Sr I-B,M and N.Actual RFLP analysis of both enzymes Bst U I and Bfa I confirmed that of the virtual RFLP analysis.Combining these results,we concluded that Po F was a member of the‘Candidatus Phytoplasma asteris’group(16Sr I),and has very close relationship with 16Sr I-B subgroup.
