The transcriptomes of three different parts of the fertile tetrasporophyte of Gracilariopsis lemaneiformis,including tip(T),middle(M),and subjacent(S)parts,with a gradual tetrasporangium maturity were analyzed and com...The transcriptomes of three different parts of the fertile tetrasporophyte of Gracilariopsis lemaneiformis,including tip(T),middle(M),and subjacent(S)parts,with a gradual tetrasporangium maturity were analyzed and compared to identify the genes involved in the process of tetrasporogenesis.The number of differentially expressed genes(DEGs)for the Gple-S versus Gple-T comparison was 10296,and the numbers of DEGs for the Gple-S versus Gple-M and Gple-T versus Gple-M comparisons were 7435 and 1337,respectively.Gene Ontology and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analyses were performed,and the results showed the enrichment of 132 KEGG pathways(corrected P<0.05).A total of 58 DEGs related to meiosis were screened and blasted against 18 meiosis-related genes(dmc1,mlh1,mnd1,msh4,msh2,msh6,mre11,pds5,pms1,rad21,rad50,rad51,smc1,smc2,smc4,smc5,smc6,and spo11),including four meiosis-specific genes.The transcriptome comparison indicated that in the T part,the meiosis,ribosome,and RNA transport-related genes were mostly up-regulated compared with those in the other two groups.In the M part,the genes related to ribosomes and the endoplasmic reticulum were also up-regulated compared with those in the lower part.Finally,in the S part,the genes associated with photosynthesis were mostly up-regulated,which might be helpful to the recovery from spore formation and release.展开更多
目的:分析对失眠伴抑郁障碍患者应用针灸治疗的效果。方法:将2019年6月-2020年9月在南京中医药大学附属昆山市中医医院接受治疗的90例失眠伴抑郁障碍患者作为本次观察对象,并将所有患者按照不同的治疗方式分为观察组(45例)和对照组(45...目的:分析对失眠伴抑郁障碍患者应用针灸治疗的效果。方法:将2019年6月-2020年9月在南京中医药大学附属昆山市中医医院接受治疗的90例失眠伴抑郁障碍患者作为本次观察对象,并将所有患者按照不同的治疗方式分为观察组(45例)和对照组(45例),对照组和观察组分别采用常规药物治疗和针灸治疗,应用匹兹堡睡眠质量指数(Pittsburgh Sleep Quality Index,PSQI)、中华生存质量量表(Chinese Quality of Life Scale,ChQOL)、汉密尔顿抑郁量表(Hamilton Depression Scale,HAMD)评估两组疗效、中医证候(情绪不宁、心情抑郁、失眠)积分。结果:观察组的睡眠质量、生存质量明显比对照组好(P<0.05);治疗前观察组的抑郁评分与对照组的差异无统计学意义(P=0.728);治疗后,观察组的抑郁评分明显比对照组低(P<0.05)。结论:对失眠伴抑郁障碍患者应用针灸治疗效果显著,能够有效改善患者的生存质量和睡眠质量,缓解患者的抑郁情绪,使患者能够积极配合临床治疗,同时能以积极乐观的心态面对治疗,提高治疗依从性,相较于常规干预措施更能减轻患者的中医证候积分,有效提高治疗效果,值得推广应用。展开更多
基金This research was supported by the Key Program of Science and Technology Innovation in Ningbo(No.2019B10009)the National Natural Science Foundation of China(No.41476111).
文摘The transcriptomes of three different parts of the fertile tetrasporophyte of Gracilariopsis lemaneiformis,including tip(T),middle(M),and subjacent(S)parts,with a gradual tetrasporangium maturity were analyzed and compared to identify the genes involved in the process of tetrasporogenesis.The number of differentially expressed genes(DEGs)for the Gple-S versus Gple-T comparison was 10296,and the numbers of DEGs for the Gple-S versus Gple-M and Gple-T versus Gple-M comparisons were 7435 and 1337,respectively.Gene Ontology and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analyses were performed,and the results showed the enrichment of 132 KEGG pathways(corrected P<0.05).A total of 58 DEGs related to meiosis were screened and blasted against 18 meiosis-related genes(dmc1,mlh1,mnd1,msh4,msh2,msh6,mre11,pds5,pms1,rad21,rad50,rad51,smc1,smc2,smc4,smc5,smc6,and spo11),including four meiosis-specific genes.The transcriptome comparison indicated that in the T part,the meiosis,ribosome,and RNA transport-related genes were mostly up-regulated compared with those in the other two groups.In the M part,the genes related to ribosomes and the endoplasmic reticulum were also up-regulated compared with those in the lower part.Finally,in the S part,the genes associated with photosynthesis were mostly up-regulated,which might be helpful to the recovery from spore formation and release.
文摘目的:分析对失眠伴抑郁障碍患者应用针灸治疗的效果。方法:将2019年6月-2020年9月在南京中医药大学附属昆山市中医医院接受治疗的90例失眠伴抑郁障碍患者作为本次观察对象,并将所有患者按照不同的治疗方式分为观察组(45例)和对照组(45例),对照组和观察组分别采用常规药物治疗和针灸治疗,应用匹兹堡睡眠质量指数(Pittsburgh Sleep Quality Index,PSQI)、中华生存质量量表(Chinese Quality of Life Scale,ChQOL)、汉密尔顿抑郁量表(Hamilton Depression Scale,HAMD)评估两组疗效、中医证候(情绪不宁、心情抑郁、失眠)积分。结果:观察组的睡眠质量、生存质量明显比对照组好(P<0.05);治疗前观察组的抑郁评分与对照组的差异无统计学意义(P=0.728);治疗后,观察组的抑郁评分明显比对照组低(P<0.05)。结论:对失眠伴抑郁障碍患者应用针灸治疗效果显著,能够有效改善患者的生存质量和睡眠质量,缓解患者的抑郁情绪,使患者能够积极配合临床治疗,同时能以积极乐观的心态面对治疗,提高治疗依从性,相较于常规干预措施更能减轻患者的中医证候积分,有效提高治疗效果,值得推广应用。
