3´,5´-Cyclic adenosine monophosphate(cAMP)is an important metabolite that is specifically enriched in jujube.However,the effect of cAMP on jujube cellular responses has not been comprehensively studied.Here,...3´,5´-Cyclic adenosine monophosphate(cAMP)is an important metabolite that is specifically enriched in jujube.However,the effect of cAMP on jujube cellular responses has not been comprehensively studied.Here,we established jujube cell suspension cultures and investigated the calcium influx in response to cAMP treatment through protoplast isolation and fluorescence intensity.Firstly,cAMP treatment could promote jujube growth and increase the content of endogenous cAMP.Using transcriptome analysis with transgenic Arabidopsis plants overexpressing adenylate cyclase(ZjAC)as a positive control,we identified 60 calcium-related differential expressed genes(DEGs)that contributed to the calcium signaling and inter-or intra-cellular responses.Pharmacological treatments such as cAMP and the calcium ionophore A23187 could induce ZjAC expression,the accumulation of cAMP and calcium influx in jujube cells,while ethylene glycol tetraacetic acid(EGTA)or bithionol treatment inhibited these changes.Moreover,the calcium channels and transporters in calcium influx,such as the ZjCNGC2 channel and the mitogen activated protein(MAP)kinase pathway,could be activated by cAMP treatment.In summary,our findings demonstrated that cAMP biosynthesis is dependent on calcium influx and the amplifying effect between calcium and cAMP may be involved in intracellular signal induction,which might contribute to the growth and development of jujube.展开更多
目的探讨椎间孔镜下改良TESSYS技术联合270°神经根减压治疗腰椎管狭窄症的临床疗效。方法回顾性分析三门峡市中心医院2012年1月至2015年1月收治的123例腰椎管狭窄症患者的临床资料,其中行经椎间孔腰椎椎体间融合术(TLIF)治疗61例(...目的探讨椎间孔镜下改良TESSYS技术联合270°神经根减压治疗腰椎管狭窄症的临床疗效。方法回顾性分析三门峡市中心医院2012年1月至2015年1月收治的123例腰椎管狭窄症患者的临床资料,其中行经椎间孔腰椎椎体间融合术(TLIF)治疗61例(对照组),行椎间孔镜下改良TESSYS技术联合270°神经根减压治疗62例(观察组),比较2组患者手术创面面积、术中出血量、手术时间、卧床时间及住院费用;术前及术后7 d和3、6、12个月,使用视觉疼痛模拟评分(VAS)法评估患者术后腰腿疼痛情况,使用OSWESTRY功能障碍指数(ODI)评估患者腰椎功能改善情况。结果与对照组比较,观察组患者手术创面面积小(P<0.05),术中出血量少(P<0.05),手术时间、卧床时间短(P<0.05),住院费用低(P<0.05)。2组患者术前VAS评分和ODI比较差异无统计学意义(P>0.05);观察组患者术后7 d VAS评分和ODI显著低于对照组(P<0.05);而术后3、6、12个月2组患者VAS评分和ODI比较差异无统计学意义(P>0.05)。结论椎间孔镜下改良TESSYS技术联合270°神经根减压治疗腰椎管狭窄症具有手术创面面积小、术中出血量少、手术时间短、住院费用低等优点。展开更多
基金This research was funded by the Provincial Supporting Program of Hebei for the Returned Oversea Scholars,China(C20210114)the Science and Technology Project of Hebei Education Department(QN2022017)+5 种基金the Fundamental Scientific Research Fund of Universities in Hebei Province(KY2021059)the China Agriculture Research System(CARS-30-2-07)the National Key Research and Development Project of China(2019YFD1001605)the Natural Science Foundation of Hebei Province(C2020204082)the Funds for Hebei Jujube Industry Technology Research Institute after Operation Performance(205676155H)the Young Talent Project of Hebei Agricultural University Foundation(YJ201853).
文摘3´,5´-Cyclic adenosine monophosphate(cAMP)is an important metabolite that is specifically enriched in jujube.However,the effect of cAMP on jujube cellular responses has not been comprehensively studied.Here,we established jujube cell suspension cultures and investigated the calcium influx in response to cAMP treatment through protoplast isolation and fluorescence intensity.Firstly,cAMP treatment could promote jujube growth and increase the content of endogenous cAMP.Using transcriptome analysis with transgenic Arabidopsis plants overexpressing adenylate cyclase(ZjAC)as a positive control,we identified 60 calcium-related differential expressed genes(DEGs)that contributed to the calcium signaling and inter-or intra-cellular responses.Pharmacological treatments such as cAMP and the calcium ionophore A23187 could induce ZjAC expression,the accumulation of cAMP and calcium influx in jujube cells,while ethylene glycol tetraacetic acid(EGTA)or bithionol treatment inhibited these changes.Moreover,the calcium channels and transporters in calcium influx,such as the ZjCNGC2 channel and the mitogen activated protein(MAP)kinase pathway,could be activated by cAMP treatment.In summary,our findings demonstrated that cAMP biosynthesis is dependent on calcium influx and the amplifying effect between calcium and cAMP may be involved in intracellular signal induction,which might contribute to the growth and development of jujube.
文摘目的探讨椎间孔镜下改良TESSYS技术联合270°神经根减压治疗腰椎管狭窄症的临床疗效。方法回顾性分析三门峡市中心医院2012年1月至2015年1月收治的123例腰椎管狭窄症患者的临床资料,其中行经椎间孔腰椎椎体间融合术(TLIF)治疗61例(对照组),行椎间孔镜下改良TESSYS技术联合270°神经根减压治疗62例(观察组),比较2组患者手术创面面积、术中出血量、手术时间、卧床时间及住院费用;术前及术后7 d和3、6、12个月,使用视觉疼痛模拟评分(VAS)法评估患者术后腰腿疼痛情况,使用OSWESTRY功能障碍指数(ODI)评估患者腰椎功能改善情况。结果与对照组比较,观察组患者手术创面面积小(P<0.05),术中出血量少(P<0.05),手术时间、卧床时间短(P<0.05),住院费用低(P<0.05)。2组患者术前VAS评分和ODI比较差异无统计学意义(P>0.05);观察组患者术后7 d VAS评分和ODI显著低于对照组(P<0.05);而术后3、6、12个月2组患者VAS评分和ODI比较差异无统计学意义(P>0.05)。结论椎间孔镜下改良TESSYS技术联合270°神经根减压治疗腰椎管狭窄症具有手术创面面积小、术中出血量少、手术时间短、住院费用低等优点。
