A method for determination of astaxanthin and astaxanthin eaters in shrimp shell by high performance liquid chromatography is established.Shrimp shell are addressed with 5%hydrochloric acid to remove calcium ions.Afte...A method for determination of astaxanthin and astaxanthin eaters in shrimp shell by high performance liquid chromatography is established.Shrimp shell are addressed with 5%hydrochloric acid to remove calcium ions.After shell is dried,organics from shrimp shell are extracted with anhydrous ethanol.The alcohol extrative of the shrimp shell is mixed with the ammonium sulfate to extract astaxanthin by aqueous two-phase extraction.The crude astaxanthin is collected,which is distributed in the middle layer of the aqueous two-phase layer.After distilled water is added to the crude astaxanthin,the aqueous solution is centrifuged,and the previous step is repeated for several times.The precipitation in centrifuge tube is collected and dried.The crude astaxanthin dried is dissolved with acetone,and the sample solution is separated by TLC.Every pigment on the TLC plate is collected and dissolved with acetone.The pigments are determined by high performance liquid chromatograph.The results show that aqueous two-phase system,3 mL alcohol extractive of astaxanthin and 4.5 mL 20%ammonium sulfate,can be used to acquire crude astaxanthin.The wavelength of the maximum peak of astaxanthin in ethanol solution is 472 nm.A variety of pigments can be separated from the crude astaxanthin by thin-layer chromatography,including free astaxanthin,astaxanthin monoester,astaxanthin diester,echinenone and other substances.It can be seen from high performance liquid chromatography that the appearance time of free astaxanthin is from 4 min to 5.5 min,and the appearance time of astaxanthin monoester is from 10.5 min to 27.8 min.The method is simple about the sample pretreatment and feasible about the determination of astaxanthin and astaxanthin esters in shrimp shell.展开更多
Marine phytoplankton plays a very important role in marine ecology,environment and global climate change,and it is an indicative organism for measuring water quality.The Bohai Sea and the Yellow Sea in China have uniq...Marine phytoplankton plays a very important role in marine ecology,environment and global climate change,and it is an indicative organism for measuring water quality.The Bohai Sea and the Yellow Sea in China have unique geographical locations.Therefore,the characteristics of phytoplankton population distribution in the Bohai Sea and the Yellow Sea are of great significance to the study of marine ecology in China.In this work,the pigment data obtained from the Yellow Sea and Bohai Sea in August 2015 were analyzed by HPLC(High-Performance Liquid Chromatography).Then the pigment data were analyzed statistically by the CHEMTAX software,so that the phytoplankton community structure information was obtained.Results show that in summer 2015,from the perspective of sea area,the biomass of phytoplankton in the surface of Bohai Sea is higher than that in the Yellow Sea,while the phytoplankton biomass in the surface of North Yellow Sea is higher than that in the South.From the perspective of dominant species of phytoplankton,the surface waters of the Yellow Sea and Bohai Sea were dominated by diatoms,prymnesiophytes and chlorophytes,accounting for 55.76%,14.56%and 14.55%respectively.Among them,diatoms accounted for the absolute advantage.展开更多
基金supported by Liao Ning Revitalization Talents Program No. XLYC1807161Dalian High-level Talents Innovation Support Plan No. 2017RQ063+4 种基金Dalian Ocean University Zhanlan scholar ProgramThe National Natural Science Foundation of China under contract Nos. 41206013, 41430963the Public Science and Technology Research Funds Projects of Ocean under contract No. 201205018the National Science and Technology Support Program under contract No. 2014BAB12B02Projects of Institute of Marine Industry Technology of Liaoning Universities
文摘A method for determination of astaxanthin and astaxanthin eaters in shrimp shell by high performance liquid chromatography is established.Shrimp shell are addressed with 5%hydrochloric acid to remove calcium ions.After shell is dried,organics from shrimp shell are extracted with anhydrous ethanol.The alcohol extrative of the shrimp shell is mixed with the ammonium sulfate to extract astaxanthin by aqueous two-phase extraction.The crude astaxanthin is collected,which is distributed in the middle layer of the aqueous two-phase layer.After distilled water is added to the crude astaxanthin,the aqueous solution is centrifuged,and the previous step is repeated for several times.The precipitation in centrifuge tube is collected and dried.The crude astaxanthin dried is dissolved with acetone,and the sample solution is separated by TLC.Every pigment on the TLC plate is collected and dissolved with acetone.The pigments are determined by high performance liquid chromatograph.The results show that aqueous two-phase system,3 mL alcohol extractive of astaxanthin and 4.5 mL 20%ammonium sulfate,can be used to acquire crude astaxanthin.The wavelength of the maximum peak of astaxanthin in ethanol solution is 472 nm.A variety of pigments can be separated from the crude astaxanthin by thin-layer chromatography,including free astaxanthin,astaxanthin monoester,astaxanthin diester,echinenone and other substances.It can be seen from high performance liquid chromatography that the appearance time of free astaxanthin is from 4 min to 5.5 min,and the appearance time of astaxanthin monoester is from 10.5 min to 27.8 min.The method is simple about the sample pretreatment and feasible about the determination of astaxanthin and astaxanthin esters in shrimp shell.
基金supported by Liao Ning Revitalization Talents Program No. XLYC1807161Dalian High-level Talents Innovation Support Plan No. 2017RQ063+4 种基金Dalian Ocean University “Zhanlan scholar”ProgramThe National Natural Science Foundation of China under contract Nos. 41206013 and 41430963the Public Science and Technology Research Funds Projects of Ocean under contract No. 201205018the National Science and Technology Support Program under contract No. 2014BAB12B02Projects of Institute of Marine Industry Technology of Liaoning Universities
文摘Marine phytoplankton plays a very important role in marine ecology,environment and global climate change,and it is an indicative organism for measuring water quality.The Bohai Sea and the Yellow Sea in China have unique geographical locations.Therefore,the characteristics of phytoplankton population distribution in the Bohai Sea and the Yellow Sea are of great significance to the study of marine ecology in China.In this work,the pigment data obtained from the Yellow Sea and Bohai Sea in August 2015 were analyzed by HPLC(High-Performance Liquid Chromatography).Then the pigment data were analyzed statistically by the CHEMTAX software,so that the phytoplankton community structure information was obtained.Results show that in summer 2015,from the perspective of sea area,the biomass of phytoplankton in the surface of Bohai Sea is higher than that in the Yellow Sea,while the phytoplankton biomass in the surface of North Yellow Sea is higher than that in the South.From the perspective of dominant species of phytoplankton,the surface waters of the Yellow Sea and Bohai Sea were dominated by diatoms,prymnesiophytes and chlorophytes,accounting for 55.76%,14.56%and 14.55%respectively.Among them,diatoms accounted for the absolute advantage.
