Sugar content is a determinant of apple(Malus×domestica Borkh.)sweetness.However,the molecular mechanism underlying sucrose accumulation in apple fruit remains elusive.Herein,this study reported the role of the s...Sugar content is a determinant of apple(Malus×domestica Borkh.)sweetness.However,the molecular mechanism underlying sucrose accumulation in apple fruit remains elusive.Herein,this study reported the role of the sucrose transporter MdSUT2.1 in the regulation of sucrose accumulation in apples.The MdSUT2.1 gene encoded a protein with 612 amino acid residues that could be localized at the plasma membrane when expressed in tobacco leaf protoplasts.MdSUT2.1 was highly expressed in fruit and was positively correlated with sucrose accumulation during apple fruit development.Moreover,complementary growth assays in a yeast mutant validated the sucrose transport activity of MdSUT2.1.MdSUT2.1 overexpression in apples and tomatoes resulted in significant increases in sucrose,fructose,and glucose contents compared to the wild type(WT).Further analysis revealed that the expression levels of sugar metabolism-and transport-related genes SUSYs,NINVs,FRKs,HXKs,and TSTs increased in apples and tomatoes with MdSUT2.1 overexpression compared to WT.Finally,unlike the tonoplast sugar transporters MdTST1 and MdTST2,the promoter of MdSUT2.1 was not induced by exogenous sugars.These findings provide valuable insights into the molecular mechanism underlying sugar accumulation in apples.展开更多
目的:描述并比较使用及未使用院前急救医疗服务(emergency medical services,EMS)的急性缺血性脑卒中(acute ischemic stroke,AIS)患者的诊疗方式及诊疗过程。方法:回顾性分析广州市番禺区中心医院自2019年10月至2020年11月救治的1041例...目的:描述并比较使用及未使用院前急救医疗服务(emergency medical services,EMS)的急性缺血性脑卒中(acute ischemic stroke,AIS)患者的诊疗方式及诊疗过程。方法:回顾性分析广州市番禺区中心医院自2019年10月至2020年11月救治的1041例AIS患者。结果:与自行入院者相比,救护车运送入院者年龄较更高,患者出现言语不清的症状更多,入院NIHSS得分更高,从发病到入院时间(time from onset to hospital admission,OTD)时间更短,接受静脉溶栓、血管内介入治疗的比例更高,从入门到开始CT时间(door to CT scan,DTC)时间更短,均具有统计学意义。结论:接受EMS的AIS患者更有利于救治工作的开展。展开更多
基金supported by the earmarked fund for China Agriculture Research System(CARS-27)。
文摘Sugar content is a determinant of apple(Malus×domestica Borkh.)sweetness.However,the molecular mechanism underlying sucrose accumulation in apple fruit remains elusive.Herein,this study reported the role of the sucrose transporter MdSUT2.1 in the regulation of sucrose accumulation in apples.The MdSUT2.1 gene encoded a protein with 612 amino acid residues that could be localized at the plasma membrane when expressed in tobacco leaf protoplasts.MdSUT2.1 was highly expressed in fruit and was positively correlated with sucrose accumulation during apple fruit development.Moreover,complementary growth assays in a yeast mutant validated the sucrose transport activity of MdSUT2.1.MdSUT2.1 overexpression in apples and tomatoes resulted in significant increases in sucrose,fructose,and glucose contents compared to the wild type(WT).Further analysis revealed that the expression levels of sugar metabolism-and transport-related genes SUSYs,NINVs,FRKs,HXKs,and TSTs increased in apples and tomatoes with MdSUT2.1 overexpression compared to WT.Finally,unlike the tonoplast sugar transporters MdTST1 and MdTST2,the promoter of MdSUT2.1 was not induced by exogenous sugars.These findings provide valuable insights into the molecular mechanism underlying sugar accumulation in apples.
文摘目的:描述并比较使用及未使用院前急救医疗服务(emergency medical services,EMS)的急性缺血性脑卒中(acute ischemic stroke,AIS)患者的诊疗方式及诊疗过程。方法:回顾性分析广州市番禺区中心医院自2019年10月至2020年11月救治的1041例AIS患者。结果:与自行入院者相比,救护车运送入院者年龄较更高,患者出现言语不清的症状更多,入院NIHSS得分更高,从发病到入院时间(time from onset to hospital admission,OTD)时间更短,接受静脉溶栓、血管内介入治疗的比例更高,从入门到开始CT时间(door to CT scan,DTC)时间更短,均具有统计学意义。结论:接受EMS的AIS患者更有利于救治工作的开展。
