目的分析先天性心脏病介入治疗前后心律失常及心率变异性(heart rate variability,HRV)变化与术后心功能的相关性。方法选取2016年3月至2019年3月在徐州市中心医院接受介入治疗的268例先天性心脏病患者,观察患者治疗前后心律失常的类型...目的分析先天性心脏病介入治疗前后心律失常及心率变异性(heart rate variability,HRV)变化与术后心功能的相关性。方法选取2016年3月至2019年3月在徐州市中心医院接受介入治疗的268例先天性心脏病患者,观察患者治疗前后心律失常的类型和检出率,HRV的时域指标[正常R-R间期平均值(normal R-R interval average,R-R)、相邻正常心动周期≥50ms的R-R间期差值构成比(composition ratio of R-R interval difference between adjacent normal cardiac cycles≥50ms,PNN50%)、24h内每5min节段R-R间期均值标准差(standard deviation of mean R-R interval per 5min segment within 24h,ASDNN)、24h内每5min节段R-R间期平均标准差(mean standard deviation of R-R interval per 5min segment within 24h,SDANN)、24h内R-R间期标准差(standard deviation of R-R interval within 24h,SDNN)、相邻正常R-R间期标准差(adjacent normal R-R interval standard deviation,rMSSD)]及频域指标[总功率(total power,TP)、低频功率(low frequency power,LF)、高频功率(high frequency power,HF)、低频功率/高频功率比值(LF/HF)]的变化,并分析其与术后心功能的相关性。结果治疗后患者心律失常的总检出率为69.03%,明显高于治疗前的51.49%,差异有显著性(P<0.05)。治疗后患者HRV时域和频域指标与治疗前比较明显改善(P<0.05)。心功能Ⅲ级和Ⅳ级患者HRV的时域和频域指标与心功能Ⅰ级和Ⅱ级患者比较差异有显著性(P<0.05)。相关分析显示,心律失常检出率、R-R、PNN50%、ASDNN、SDANN、SDNN、rMSSD、TP、LF、HF、LF/HF与患者术后心功能呈明显相关关系(Spearman等级相关系数Rs=-0.361、0.536、0.416、0.468、0.408、0.438、0.368、0.514、0.317、0.386、0.421,P<0.05),而心律失常类型与患者术后心功能无明显相关性(Rs=0.053,P=0.518)。结论先天性心脏病介入治疗后心律失常发生率及HRV的时域和频域指标明显增高,并与患者术后心功能相关,可作为判定心功能康复的参考指标。展开更多
Objective: To investigate the potential efficacy of panaxadiol saponins component(PDS-C) in the treatment of aplastic anemia(AA) model mice. Methods: Totally 70 mice were divided into 7 groups as follows: normal, mode...Objective: To investigate the potential efficacy of panaxadiol saponins component(PDS-C) in the treatment of aplastic anemia(AA) model mice. Methods: Totally 70 mice were divided into 7 groups as follows: normal, model, low-, medium-, high-dose PDS-C(20, 40, 80 mg/kg, namely L-, M-, H-PDS-C), cyclosporine(40 mg/kg), and andriol(25 mg/kg) groups, respectively. An immune-mediated AA mouse model was established in BALB/c mice by exposing to 5.0 Gy total body irradiation at 1.0 Gy/min, and injecting with lymphocytes from DBA mice. On day 4 after establishment of AA model, all drugs were intragastrically administered daily for 15 days, respectively, while the mice in the normal and model groups were administered with saline solution. After treatment, the peripheral blood counts, bone marrow pathological examination, colony forming assay of bone marrow culture, T lymphocyte subpopulation analysis, as well as T-bet, GATA-3 and Fox P3 proteins were detected by flow cytometry and Western blot. Results: The peripheral blood of white blood cell(WBC), platelet, neutrophil counts and hemoglobin(Hb) concentration were significantly decreased in the model group compared with the normal group(all P<0.01). In response to 3 dose PDS-C treatment, the WBC, platelet, neutrophil counts were significantly increased at a dose-dependent manner compared with the model group(all P<0.01). The myelosuppression status of AA was significantly reduced in M-, H-PDS-C groups, and hematopoietic cell quantity of bone marrow was more abundant than the model group. The colony numbers of myeloid, erythroid and megakaryocytic progenitor cells in the model group were less than those of the normal mice in bone marrow culture, while, PDS-C therapy enhanced proliferation of hematopoietic progenitor cells by significantly increasing colony numbers(all P<0.01). Furthermore,PDS-C therapy increased peripheral blood CD3^+ and CD3^+CD4^+ cells and reduced CD3^+CD8^+ cells(P<0.05 or P<0.01). Meanwhile, PDS-C treatment at medium-and high doses groups also increased CD4^+CD25^+Fox P3^+ cells, downregulated T-bet protein expression, and upregulated GATA-3 and Fox P3 protein expressions in spleen cells(P<0.05). Conclusion: PDS-C possesses dual activities, promoting proliferation hematopoietic progenitor cells and modulating T lymphocyte immune functions in the treatment of AA model mice.展开更多
Graft-versus-host disease(GVHD)is the most common complication after allogeneic hematopoietic stem cell transplantation,and also an important factor affecting the survival and quality of life in patients after transpl...Graft-versus-host disease(GVHD)is the most common complication after allogeneic hematopoietic stem cell transplantation,and also an important factor affecting the survival and quality of life in patients after transplantation.Currently,immunosuppressive therapy is commonly used for GVHD,but the curative effect is not ideal.How to effectively prevent and treat GVHD is one of the difficulties to be solved urgently in the field of transplantation.In this paper,we summarize the latest progress in pathogenesis,prevention and treatment of GVHD with Chinese medicine(CM).We hope it will provide ideas and methods for exploring the mechanism and establishing a new comprehensive therapy for GVHD with CM.展开更多
文摘目的分析先天性心脏病介入治疗前后心律失常及心率变异性(heart rate variability,HRV)变化与术后心功能的相关性。方法选取2016年3月至2019年3月在徐州市中心医院接受介入治疗的268例先天性心脏病患者,观察患者治疗前后心律失常的类型和检出率,HRV的时域指标[正常R-R间期平均值(normal R-R interval average,R-R)、相邻正常心动周期≥50ms的R-R间期差值构成比(composition ratio of R-R interval difference between adjacent normal cardiac cycles≥50ms,PNN50%)、24h内每5min节段R-R间期均值标准差(standard deviation of mean R-R interval per 5min segment within 24h,ASDNN)、24h内每5min节段R-R间期平均标准差(mean standard deviation of R-R interval per 5min segment within 24h,SDANN)、24h内R-R间期标准差(standard deviation of R-R interval within 24h,SDNN)、相邻正常R-R间期标准差(adjacent normal R-R interval standard deviation,rMSSD)]及频域指标[总功率(total power,TP)、低频功率(low frequency power,LF)、高频功率(high frequency power,HF)、低频功率/高频功率比值(LF/HF)]的变化,并分析其与术后心功能的相关性。结果治疗后患者心律失常的总检出率为69.03%,明显高于治疗前的51.49%,差异有显著性(P<0.05)。治疗后患者HRV时域和频域指标与治疗前比较明显改善(P<0.05)。心功能Ⅲ级和Ⅳ级患者HRV的时域和频域指标与心功能Ⅰ级和Ⅱ级患者比较差异有显著性(P<0.05)。相关分析显示,心律失常检出率、R-R、PNN50%、ASDNN、SDANN、SDNN、rMSSD、TP、LF、HF、LF/HF与患者术后心功能呈明显相关关系(Spearman等级相关系数Rs=-0.361、0.536、0.416、0.468、0.408、0.438、0.368、0.514、0.317、0.386、0.421,P<0.05),而心律失常类型与患者术后心功能无明显相关性(Rs=0.053,P=0.518)。结论先天性心脏病介入治疗后心律失常发生率及HRV的时域和频域指标明显增高,并与患者术后心功能相关,可作为判定心功能康复的参考指标。
基金Supported by the National Natural Science Foundation of China(No.81774068)Medical and Health Key Project of Zhejiang Province(No.2011ZDA021)Zhejiang Provincial Natural Science Foundation of China(No.LY14H280004)
文摘Objective: To investigate the potential efficacy of panaxadiol saponins component(PDS-C) in the treatment of aplastic anemia(AA) model mice. Methods: Totally 70 mice were divided into 7 groups as follows: normal, model, low-, medium-, high-dose PDS-C(20, 40, 80 mg/kg, namely L-, M-, H-PDS-C), cyclosporine(40 mg/kg), and andriol(25 mg/kg) groups, respectively. An immune-mediated AA mouse model was established in BALB/c mice by exposing to 5.0 Gy total body irradiation at 1.0 Gy/min, and injecting with lymphocytes from DBA mice. On day 4 after establishment of AA model, all drugs were intragastrically administered daily for 15 days, respectively, while the mice in the normal and model groups were administered with saline solution. After treatment, the peripheral blood counts, bone marrow pathological examination, colony forming assay of bone marrow culture, T lymphocyte subpopulation analysis, as well as T-bet, GATA-3 and Fox P3 proteins were detected by flow cytometry and Western blot. Results: The peripheral blood of white blood cell(WBC), platelet, neutrophil counts and hemoglobin(Hb) concentration were significantly decreased in the model group compared with the normal group(all P<0.01). In response to 3 dose PDS-C treatment, the WBC, platelet, neutrophil counts were significantly increased at a dose-dependent manner compared with the model group(all P<0.01). The myelosuppression status of AA was significantly reduced in M-, H-PDS-C groups, and hematopoietic cell quantity of bone marrow was more abundant than the model group. The colony numbers of myeloid, erythroid and megakaryocytic progenitor cells in the model group were less than those of the normal mice in bone marrow culture, while, PDS-C therapy enhanced proliferation of hematopoietic progenitor cells by significantly increasing colony numbers(all P<0.01). Furthermore,PDS-C therapy increased peripheral blood CD3^+ and CD3^+CD4^+ cells and reduced CD3^+CD8^+ cells(P<0.05 or P<0.01). Meanwhile, PDS-C treatment at medium-and high doses groups also increased CD4^+CD25^+Fox P3^+ cells, downregulated T-bet protein expression, and upregulated GATA-3 and Fox P3 protein expressions in spleen cells(P<0.05). Conclusion: PDS-C possesses dual activities, promoting proliferation hematopoietic progenitor cells and modulating T lymphocyte immune functions in the treatment of AA model mice.
基金Supported by the National Natural Science Foundation of China(No.81774068)the Natural Science Foundation of Zhejiang Province(Youth Project,No.LQ19H290002)。
文摘Graft-versus-host disease(GVHD)is the most common complication after allogeneic hematopoietic stem cell transplantation,and also an important factor affecting the survival and quality of life in patients after transplantation.Currently,immunosuppressive therapy is commonly used for GVHD,but the curative effect is not ideal.How to effectively prevent and treat GVHD is one of the difficulties to be solved urgently in the field of transplantation.In this paper,we summarize the latest progress in pathogenesis,prevention and treatment of GVHD with Chinese medicine(CM).We hope it will provide ideas and methods for exploring the mechanism and establishing a new comprehensive therapy for GVHD with CM.
