A rice population consisting of 90 TN1/Guiyigu F3 lines was employed to analyze the linkage between DNA markers and a new gene Wbph6(t) conferring resistance to whitebacked planthopper, Sogatella furcifera By using th...A rice population consisting of 90 TN1/Guiyigu F3 lines was employed to analyze the linkage between DNA markers and a new gene Wbph6(t) conferring resistance to whitebacked planthopper, Sogatella furcifera By using the mapping approach of bulked extremes and recessive class, Wbph6(t) was mapped onto the short arm of chromosome 11 with a genetic distance of 21.2 cM to SSLP marker RM167.展开更多
By using 304 recombinant inbred lines derived from indica rice cross Zhong 156/Gumei 2, a linkage map consisting of 177 marker loci and covering 12 rice chromosomes was constructed and employed for mapping genes confe...By using 304 recombinant inbred lines derived from indica rice cross Zhong 156/Gumei 2, a linkage map consisting of 177 marker loci and covering 12 rice chromosomes was constructed and employed for mapping genes conferring blast resistance in rice. Genomic location of gene Pi25(t) conferring neck blast resistance to the Chinese isolate 92-183 (race ZC15) was verified to be located between markers A7 and RG456 on chromosome 6, with genetic distances of 1.7 cM and 1.5 cM to A7 and RG456, respectively. Leaf blast resistance of Gumei 2 to the Philippine isolate Ca89 (lineage 4) was found to be controlled by a single gene. The gene tentatively designated as Pi26(\) was located between makers B10 and R674 on chromosome 6, with genetic distances of 5.7 cM and 25.8 cM to B10 and R674 respectively. Resistant alleles at both gene loci were derived from Gumei 2, indicating an existence of resistance gene cluster in Gumei 2.展开更多
The genetic diversity of Dongxiang wild rice populations was evaluated by using 14 SSR primer pairs. Twelve of the primer pairs amplified polymorphic bands. A total of 70 polymorphic DNA bands were generated with aver...The genetic diversity of Dongxiang wild rice populations was evaluated by using 14 SSR primer pairs. Twelve of the primer pairs amplified polymorphic bands. A total of 70 polymorphic DNA bands were generated with average 5. 83 polymorphic bands per primer pair, indicating that the genetic diversity of Dongxiang wild rice was high. Cluster analysis showed that there were highly variant individuals within and among populations, while others were highly similar to each other. The within-population genetic distances were 0. 23 - 0.47, being smaller than 0.40 - 0.55 of among-population. Strict management must be taken for the preservation of Dongxiang wild rice.展开更多
文摘A rice population consisting of 90 TN1/Guiyigu F3 lines was employed to analyze the linkage between DNA markers and a new gene Wbph6(t) conferring resistance to whitebacked planthopper, Sogatella furcifera By using the mapping approach of bulked extremes and recessive class, Wbph6(t) was mapped onto the short arm of chromosome 11 with a genetic distance of 21.2 cM to SSLP marker RM167.
文摘By using 304 recombinant inbred lines derived from indica rice cross Zhong 156/Gumei 2, a linkage map consisting of 177 marker loci and covering 12 rice chromosomes was constructed and employed for mapping genes conferring blast resistance in rice. Genomic location of gene Pi25(t) conferring neck blast resistance to the Chinese isolate 92-183 (race ZC15) was verified to be located between markers A7 and RG456 on chromosome 6, with genetic distances of 1.7 cM and 1.5 cM to A7 and RG456, respectively. Leaf blast resistance of Gumei 2 to the Philippine isolate Ca89 (lineage 4) was found to be controlled by a single gene. The gene tentatively designated as Pi26(\) was located between makers B10 and R674 on chromosome 6, with genetic distances of 5.7 cM and 25.8 cM to B10 and R674 respectively. Resistant alleles at both gene loci were derived from Gumei 2, indicating an existence of resistance gene cluster in Gumei 2.
基金supposed by the National Natural Science Foundation of China(39969001)the Program Project of 973 Priority,China(2002CCC00800)+1 种基金the Talent Scientist Training Program Project of Jiangxi Province(20031)the Natural Science Foundation of Jiangxi Province(0330034).
文摘The genetic diversity of Dongxiang wild rice populations was evaluated by using 14 SSR primer pairs. Twelve of the primer pairs amplified polymorphic bands. A total of 70 polymorphic DNA bands were generated with average 5. 83 polymorphic bands per primer pair, indicating that the genetic diversity of Dongxiang wild rice was high. Cluster analysis showed that there were highly variant individuals within and among populations, while others were highly similar to each other. The within-population genetic distances were 0. 23 - 0.47, being smaller than 0.40 - 0.55 of among-population. Strict management must be taken for the preservation of Dongxiang wild rice.