While electrospinning is an effective method for fabricating nanofibers with good application prospects,controlling the length of stable section of electrospinning jet is critical and remains challenging.In the paper,...While electrospinning is an effective method for fabricating nanofibers with good application prospects,controlling the length of stable section of electrospinning jet is critical and remains challenging.In the paper,the effects of electrospinning parameters on the length of stable section have been investigated experimentally using polyacrylonitrile(PAN).The results based on single factor experiments and orthogonal experiments showed that the stable⁃jet length increased with the increase of solution concentration,receiving distance,voltage and solution flow rate,and decreased with the enhanced concentration of salt which was commonly utilized as additive to facilitate the spinning.Furthermore,experimental results based on polyethylene oxide(PEO)and polyvinyl alcohol(PVA)showed that the length of stable section changed with increasing salt concentration,and due to the parametric interaction,the change was distinct under different driving voltages or solution concentration conditions.展开更多
文摘目的阐明一种多肽标签Lucy-tag对于海七鳃鳗(Petromyzon marinus)嗅觉受体膜表达的促进作用。方法用免疫细胞化学、双荧光素酶报告基因(Luciferase)系统和钙流检测法,验证多肽标签Lucy-tag对于海七鳃鳗嗅觉受体的作用。结果多肽标签Lucy-tag促进了20个嗅觉受体中13个嗅觉受体的膜表达,并且Lucy-tag不影响嗅觉受体的固有活性,Lucy-tag也不影响G蛋白偶联受体(G protein coupled receptor,GPCR)的IP_3信号通路。结论该技术为寻找嗅觉受体的配体提供了有利的技术手段,为进一步深入研究GPCR与配体的功能奠定了良好的理论基础。
基金National Natural Science Foundation of China(Nos.51773037,51973027,51803023,and 61771123)Fundamental Research Funds for the Central Universities(Nos.2232020D⁃15,2232020A⁃08,2232020G⁃01,2232020D⁃14,and 2232018A3⁃11)+3 种基金Changjiang Youth Scholars Program of ChinaInnovation Program of Shanghai Municipal Education Commission,ChinaShanghai Sailing Program,China(No.18YF 1400400)China Postdoctoral Science Foundation(No.2018M640317)。
文摘While electrospinning is an effective method for fabricating nanofibers with good application prospects,controlling the length of stable section of electrospinning jet is critical and remains challenging.In the paper,the effects of electrospinning parameters on the length of stable section have been investigated experimentally using polyacrylonitrile(PAN).The results based on single factor experiments and orthogonal experiments showed that the stable⁃jet length increased with the increase of solution concentration,receiving distance,voltage and solution flow rate,and decreased with the enhanced concentration of salt which was commonly utilized as additive to facilitate the spinning.Furthermore,experimental results based on polyethylene oxide(PEO)and polyvinyl alcohol(PVA)showed that the length of stable section changed with increasing salt concentration,and due to the parametric interaction,the change was distinct under different driving voltages or solution concentration conditions.
