Fifty male Wistar rats were fed a standard chow diet or a high-fat （HF） diet, and different concentrations of green tea polyphenols （GTPs） （0.8, 1.6, and 3.2 g/L） were administered in the drinking water. We foun...Fifty male Wistar rats were fed a standard chow diet or a high-fat （HF） diet, and different concentrations of green tea polyphenols （GTPs） （0.8, 1.6, and 3.2 g/L） were administered in the drinking water. We found that the malondialdehyde （MDA） level in the HF diet group was significantly higher than that in the control （CON） group （P〈0.05）. Decreased peroxisome proliferato r-activated receptor （PPAR）-α and sirtuin 3 （SlRT3） expression, and increased manganese superoxide dismutase （MnSOD） acetylation levels were also detected in the HF diet group （P〈0.05）. GTP treatment upregulated SlRT3 and PPARa expression, increased the ppora mRNA level, reduced the MnSOD acetylation level, and decreased MDA production in rats fed a HF diet （P〈0.05）. No significant differences in total renal MnSOD and PPAR-y coactivator-1α （PGCI-α） expression were detected. The reduced oxidative stress detected in kidney tissues after GTP treatment was partly due to the higher SIRT3 expression, which was likely mediated by PPARα.展开更多
基金supported by a grant(No.81302423,81373007)from the National Natural Science Foundation of China
文摘Fifty male Wistar rats were fed a standard chow diet or a high-fat （HF） diet, and different concentrations of green tea polyphenols （GTPs） （0.8, 1.6, and 3.2 g/L） were administered in the drinking water. We found that the malondialdehyde （MDA） level in the HF diet group was significantly higher than that in the control （CON） group （P〈0.05）. Decreased peroxisome proliferato r-activated receptor （PPAR）-α and sirtuin 3 （SlRT3） expression, and increased manganese superoxide dismutase （MnSOD） acetylation levels were also detected in the HF diet group （P〈0.05）. GTP treatment upregulated SlRT3 and PPARa expression, increased the ppora mRNA level, reduced the MnSOD acetylation level, and decreased MDA production in rats fed a HF diet （P〈0.05）. No significant differences in total renal MnSOD and PPAR-y coactivator-1α （PGCI-α） expression were detected. The reduced oxidative stress detected in kidney tissues after GTP treatment was partly due to the higher SIRT3 expression, which was likely mediated by PPARα.
