纤维蛋白粘合剂粘贴双层基质透镜行兔板层角膜移植术

目的:研究纤维蛋白粘合剂(FS)粘贴的双层角膜基质透镜体内生物相容性,探讨使用该种材料行角膜移植的可行性。方法:选取健康清洁级新西兰白兔15只,采用自身对照,以兔右眼为实验眼,左眼为对照眼。实验眼使用FS粘贴的双层角膜基质透镜作为移植材料行板层角膜移植手术,对照眼不进行人工干预。分别于术后7、14、28d使用手持裂隙灯观察双眼角膜情况,并进行生物相容性评分,同时取双眼角膜行HE染色进行组织病理学检测,观察角膜恢复情况。结果:裂隙灯观察结果显示,至角膜移植术后28d,实验眼角膜上皮生长情况良好,角膜透明度基本恢复,水肿程度减轻,新生血管生长至角膜缘后未加重,未见上皮、内皮排斥线等排斥反应;对照眼角膜透明,角膜上皮光滑。生物相容性评分结果显示,角膜移植术后实验眼角膜植片水肿程度逐渐减轻,透明度逐渐恢复,排斥反应较小,角膜植片的生物相容性较好;至术后28d,实验眼和对照眼角膜透明度、水肿程度及新生血管生长程度均无差异(P>0.01)。组织病理学检测结果显示,至角膜移植术后28d,实验眼植片表面有4~5层角膜上皮细胞覆盖,角膜胶原排列整齐、规则,植片内未见明显炎性细胞浸润,双片透镜间分界消失,层间FS被机体完全吸收,植片与植床融合,未见明显分界。结论:使用FS粘贴的双层角膜基质透镜作为植片行板层角膜移植术后恢复较好,排斥反应较小,生物相容性较好,可用于板层角膜移植。

Differentiation of human olfactory mucosa mesenchymal stem cells into photoreceptor cells in vitro

AIM：To investigate whether the human olfactory mucosa mesenchymal stem cells（OM-MSCs）can differentiate into photoreceptor cells in vitro.METHODS：Through the olfactory mucosa adherent method,olfactory mucosa was isolated,cultured and identified in vitro among mesenchymal stem cells.The cell surface markers were analyzed by flow cytometry,induced to differentiate into retinal photoreceptor cells in vitro,and the expression of rhodopsin was observed and identified by Immunofluorescence and Western blot methods.RESULTS：OM-MSCs from human were spindle cellbased,and showing radial colony arrangement.OM-MSCs were negative for CD34,CD45 and CD105,but positive for CD73 and CD90.Following induction,a strong positive reaction was produced by photoreceptor specific marker rhodopsin in the cells.CONSLUSION：This novel finding demonstrates that OM-MSCs can be cultured and expanded in vitro.They possess biological characteristics of mesenchymal stem cells,and have the ability to be induced into retinal cells.