Detection of Tocopherol in Oilseed Rape (Brassica napus L.) Using Gas Chromatography with Flame Ionization Detector

The variation among Chinese genotypes of Brassica napus L. for seed tocopherols content and their analysis using gas chromatography has not been comprehensively reported till to date. In the present study, the tocopherol contents of four Chinese genotypes of Brassica napus L., namely, Gaoyou 605, Zhejiang 619, Zheshuang 758, and Zheshuang 72, were evaluated using three modified sample preparation protocols （P1, P2, and P3） for tocopherol extraction. These methods were distinguished as follows. Protocol one （P1） included the evaporation of solvent after extraction without silylation. Protocol two （P2） followed the direct supernatant collection after overnight extraction without drying and silylation. Protocol three （P3） included trimethylsilylation with N,O-bis（trimethylsilyl） trifluoroacetamide. Genotypic comparison of tocopherol and its isoforms revealed that Gaoyou 605 was dominant over the other genotypes with （140.5＋ 10.5）, （316.2＋ 9.2）, and （559.1＋ 24.3） ~tg g-~ of seed meal ct-, 7-, and total （T-） tocopherol, respectively, and a 0.44＋0.04 ^- to 7-tocopherol ratio. The comparison of the sample preparation protocols, on the other hand, suggests that P3 is the most suitable method for the tocopherol extraction from Brassica oilseeds and for the analysis of tocopherols using gas chromatography flame ionization detector （GC-FID）. Trimethylsilylation is the key step differentiating P3 from P1 and P2. Variations detected in tocopherol contents among the Chinese rapeseed （B. napus） genotypes signify the need to quantify a wide range of rapeseed germplasm for seed tocopherol dynamics in short and crop improvement in long.

茉莉酸对油菜(Brassica napus L.)受镉毒害的缓解作用（英文）

目的:本研究目的在于了解:(1)喷施外源茉莉酸对受到镉胁迫油菜的作用;(2)是否茉莉酸能够通过增强气体交换,从而保护受到氧化胁迫的地上部分组织的叶绿体,进而通过减少镉的吸收来维持离子平衡;(3)是否通过喷施茉莉酸来对具有减缓镉毒害效应的抗氧化酶的活性进行调节。创新点:茉莉酸能够调节响应胁迫的抗氧化酶的活性,从而通过保护叶绿体免受活性氧(ROS)伤害而提高光合产物的能力,最大限度地缓解油菜植株受到的镉毒害。方法:(1)叶片气体交换;(2)叶片光合色素分析;(3)丙二醛与抗氧化酶活性分析;(4)营养成分分析;(5)透射电镜亚细胞水平观察。结论:茉莉酸对于植物受镉毒害的缓解作用的机理在于减少叶片中镉的积累,从而减轻氧化胁迫过程中产生的ROS对于膜系统的损害程度。

氮肥种类与施量对油菜种子生育酚的影响(英文)

研究目的:生育酚是菜籽重要的品质指标,氮肥是影响作物生物学与籽粒产量最常用的肥料。这项研究旨在搞清楚氮肥种类与施量对油菜种子生育酚含量与组分的确切影响,以及这种影响在基因型之间的差异。创新要点:这篇论文研究了不同的氮肥种类(硝态氮与铵态氮)与低、中、高施用量对种子生育酚总量与组分的影响,并分析了其中的原因,为通过合理的氮肥施用方案配置,以达到最理想的菜籽生育酚含量或组分提供依据。研究方法:采用盆钵实验控制氮肥施量与流失的精准方法,五种基因型、二种氮肥种类、三档施量水平,三重复控制误差;尝试用气相色谱法检测菜籽生育酚含量的新方法。重要结论:尿素比硝酸氨更有利于菜籽总生育酚、阿尔法生育酚及伽马生育酚的有效形成;提高氮肥施量对于菜籽形成高含量的总生育酚与伽马生育酚非常有效,但对提高菜籽阿尔法生育酚含量的效果却不太明显。