Nitrogen is an essential nutrient for plant growth and development,and plays vital roles in crop yield.Assimilation of nitrogen is thus fine-tuned in response to heterogeneous environments.However,the regulatory mecha...Nitrogen is an essential nutrient for plant growth and development,and plays vital roles in crop yield.Assimilation of nitrogen is thus fine-tuned in response to heterogeneous environments.However,the regulatory mechanism underlying this essential process remains largely unknown.Here,we report that a zinc-finger transcription factor,drought and salt tolerance(DST),controls nitrate assimilation in rice by regulating the expression of OsNR1.2.We found that loss of function of DSTresults in a significant decrease of nitrogen use efficiency(NUE)in the presence of nitrate.Furtherstudy revealed that DST is required for full nitrate reductase activity in rice and directly regulates the expression of OsNR1.2,a gene showing sequence similarity to nitrate reductase.Reverse genetics and biochemistry studies revealed that OsNR1.2 encodes an NADH-dependent nitrate reductase that is required for high NUE of rice.Interestingly,the DST-OsNR1.2 regulatory module is involved in the suppression of nitrate assimilation under drought stress,which contributes to drought tolerance.Considering the negative role of DST in stomata closure,as revealed previously,the positive role of DST in nitrogen assimilation suggests a mechanism couplingni-trogen metabolism and stomata movement.The discovery of this coupling mechanism will aid the engi-neering of drought-tolerant crops with high NUE in the future.展开更多
Canopy photosynthesis is the sum of photosynthesis of all above-ground photosynthetic tissues.Quantitative roles of nonfoliar tissues in canopy photosynthesis remain elusive due to methodology limitations.Here,we deve...Canopy photosynthesis is the sum of photosynthesis of all above-ground photosynthetic tissues.Quantitative roles of nonfoliar tissues in canopy photosynthesis remain elusive due to methodology limitations.Here,we develop the first complete canopy photosynthesis model incorporating all above-ground photosynthetic tissues and validate this model on wheat with state-of-the-art gas exchange measurement facilities.展开更多
基金This study was funded by the Ministry of Science and Technology Key R&D program(2016YFD0100700 to D.Y.C.)Chinese Academy of Sciences(XDB27010103 to D.Y.C)National Natural Science Foundation of China(31801922 to M.L.H).
文摘Nitrogen is an essential nutrient for plant growth and development,and plays vital roles in crop yield.Assimilation of nitrogen is thus fine-tuned in response to heterogeneous environments.However,the regulatory mechanism underlying this essential process remains largely unknown.Here,we report that a zinc-finger transcription factor,drought and salt tolerance(DST),controls nitrate assimilation in rice by regulating the expression of OsNR1.2.We found that loss of function of DSTresults in a significant decrease of nitrogen use efficiency(NUE)in the presence of nitrate.Furtherstudy revealed that DST is required for full nitrate reductase activity in rice and directly regulates the expression of OsNR1.2,a gene showing sequence similarity to nitrate reductase.Reverse genetics and biochemistry studies revealed that OsNR1.2 encodes an NADH-dependent nitrate reductase that is required for high NUE of rice.Interestingly,the DST-OsNR1.2 regulatory module is involved in the suppression of nitrate assimilation under drought stress,which contributes to drought tolerance.Considering the negative role of DST in stomata closure,as revealed previously,the positive role of DST in nitrogen assimilation suggests a mechanism couplingni-trogen metabolism and stomata movement.The discovery of this coupling mechanism will aid the engi-neering of drought-tolerant crops with high NUE in the future.
基金This work was supported by the open research fund of the State Key Laboratory of Hybrid Rice(Hunan Hybrid Rice Research Center)to T.C.(2020KF01)the National Natural Science Foundation of China to T.C.(32000285)and Q.S.(31970378)+1 种基金the Chinese Academy of Science strategic lead-ing project to X.Z.(XDB27020105)the funding from the BASF Belgium Coordination Center-Innovation Center Gent to X.Z..
文摘Canopy photosynthesis is the sum of photosynthesis of all above-ground photosynthetic tissues.Quantitative roles of nonfoliar tissues in canopy photosynthesis remain elusive due to methodology limitations.Here,we develop the first complete canopy photosynthesis model incorporating all above-ground photosynthetic tissues and validate this model on wheat with state-of-the-art gas exchange measurement facilities.
