Objective:To compare effect of immunoprevention or immunotherapy based on denderitic cells (DCs),or supernatants on pancreatic carcinoma and hepatocelluar carcinoma in ritro and in vivo.Methods:DCs and monouclear cell...Objective:To compare effect of immunoprevention or immunotherapy based on denderitic cells (DCs),or supernatants on pancreatic carcinoma and hepatocelluar carcinoma in ritro and in vivo.Methods:DCs and monouclear cells (immunoeffecetor cells) were stimulated with hGM-CSF,hIL-4,hTNF-α,PC3 TA or BEL7402 TA and hIL-2,then DCs and immunoeffector cells were cocultured,and supernatants were harvested.In vitro,the immunoeffector cells were divided into A0 group (without DCs stimulated),A1 group (DCs stimulated,cultured with cytokines cocktail),A2 group (DCs stimulated,cultured with cytokines cocktail and tumor antigen.DCs vaccine).Cytoxicity assay was performed with lactate dehydogenase method,In vivo,the nude mice were allocated in 3 groups:prevention group,receiving immunoeffector cells activated by DCs vaccine 2 days before inoculation with PC3 or BEL7402;treatment group,receiving immunoeffector cells activated by DCs vaccine after development of implanted tumor in all nude mice;control group,receiving equivalents amount of RPMI1640 cultured liquid.On the 45th day,all the nude mice were sacrificed and the tumor was weighed.Results:The maximal inhibition rate of the A0.A1 andA2 were 3.5%.68.1%.81.0% in the BEL7402;4.5%,33.0%.62.4% in the PC3.The differences in tumor weight among three groups were significant,but the difference were not significant between the PC3 and BEL7402.Conclusions:DCs vaccine or supernatants may play an important role in treating and preventing against malignant tumor.展开更多
Objective:Pancreatic cancer is one of the highly malignant tumors of the digestive tract. The pathogenesis is still unclear. The clinical manifestations are mainly upper abdominal pain, jaundice, weight loss and fatig...Objective:Pancreatic cancer is one of the highly malignant tumors of the digestive tract. The pathogenesis is still unclear. The clinical manifestations are mainly upper abdominal pain, jaundice, weight loss and fatigue, and even mental and neurological disorders. However, these symptoms did not manifest in the early stage,leading to early detection, early diagnosis, early treatment, and poor prognosis. Therefore, research on clinical indicators of pancreatic cancer has become a hot topic, such as Tumor marker, albumin/globulin ratio and neutrophil/ lymphocyte ratio, collagen type VI α1 chain, constitutive photomorpho-genesis 9 signaloSome,Soluble UL16-binding protein 2 etc.These indic- ators are one of the commonly used diagnostic methods after pathology and imaging diagnosis.This article reviews and analyzes the indicators related to pancreatic cancer in recent years and reports the following.展开更多
文摘Objective:To compare effect of immunoprevention or immunotherapy based on denderitic cells (DCs),or supernatants on pancreatic carcinoma and hepatocelluar carcinoma in ritro and in vivo.Methods:DCs and monouclear cells (immunoeffecetor cells) were stimulated with hGM-CSF,hIL-4,hTNF-α,PC3 TA or BEL7402 TA and hIL-2,then DCs and immunoeffector cells were cocultured,and supernatants were harvested.In vitro,the immunoeffector cells were divided into A0 group (without DCs stimulated),A1 group (DCs stimulated,cultured with cytokines cocktail),A2 group (DCs stimulated,cultured with cytokines cocktail and tumor antigen.DCs vaccine).Cytoxicity assay was performed with lactate dehydogenase method,In vivo,the nude mice were allocated in 3 groups:prevention group,receiving immunoeffector cells activated by DCs vaccine 2 days before inoculation with PC3 or BEL7402;treatment group,receiving immunoeffector cells activated by DCs vaccine after development of implanted tumor in all nude mice;control group,receiving equivalents amount of RPMI1640 cultured liquid.On the 45th day,all the nude mice were sacrificed and the tumor was weighed.Results:The maximal inhibition rate of the A0.A1 andA2 were 3.5%.68.1%.81.0% in the BEL7402;4.5%,33.0%.62.4% in the PC3.The differences in tumor weight among three groups were significant,but the difference were not significant between the PC3 and BEL7402.Conclusions:DCs vaccine or supernatants may play an important role in treating and preventing against malignant tumor.
基金Hainan Provincial Key Research and Development Project(No.2016116).
文摘Objective:Pancreatic cancer is one of the highly malignant tumors of the digestive tract. The pathogenesis is still unclear. The clinical manifestations are mainly upper abdominal pain, jaundice, weight loss and fatigue, and even mental and neurological disorders. However, these symptoms did not manifest in the early stage,leading to early detection, early diagnosis, early treatment, and poor prognosis. Therefore, research on clinical indicators of pancreatic cancer has become a hot topic, such as Tumor marker, albumin/globulin ratio and neutrophil/ lymphocyte ratio, collagen type VI α1 chain, constitutive photomorpho-genesis 9 signaloSome,Soluble UL16-binding protein 2 etc.These indic- ators are one of the commonly used diagnostic methods after pathology and imaging diagnosis.This article reviews and analyzes the indicators related to pancreatic cancer in recent years and reports the following.
