The effect of nitrogen(N)fertilizer on the development of maize kernels has yet to be fully explored.MicroRNA-mRNA analyses could help advance our understanding of how kernels respond to N.This study analyzed the morp...The effect of nitrogen(N)fertilizer on the development of maize kernels has yet to be fully explored.MicroRNA-mRNA analyses could help advance our understanding of how kernels respond to N.This study analyzed the morphological,physiological,and transcriptomic changes in maize kernels under different N rates(0,100,200,and 300 kg ha–1).The result showed that increasing N application significantly increased maize grains’fresh and dry weight until N reached 200 kg ha–1.Higher levels of indole-3-acetic acid,cytokinin,gibberellin,and a lower level of ethylene were associated with increased N applications.We obtained 31 differentially expressed genes(DEGs)in hormone synthesis and transduction,and 9 DEGs were regulated by 14 differentially expressed microRNAs(DEMIs)in 26 pairs.The candidate DEGs and DEMIs provide valuable insight for manipulating grain filling under different N rates.展开更多
基金supported by the Major Special Research Projects in Gansu Province,China(22ZD6NA009)the State Key Laboratory of Aridland Crop Science,Gansu Agricultural University,China(GSCS-2022-Z02)+1 种基金the National Natural Science Foundation of China(32260549)the National Key R&D Program of China(2022YFD1900300)。
文摘The effect of nitrogen(N)fertilizer on the development of maize kernels has yet to be fully explored.MicroRNA-mRNA analyses could help advance our understanding of how kernels respond to N.This study analyzed the morphological,physiological,and transcriptomic changes in maize kernels under different N rates(0,100,200,and 300 kg ha–1).The result showed that increasing N application significantly increased maize grains’fresh and dry weight until N reached 200 kg ha–1.Higher levels of indole-3-acetic acid,cytokinin,gibberellin,and a lower level of ethylene were associated with increased N applications.We obtained 31 differentially expressed genes(DEGs)in hormone synthesis and transduction,and 9 DEGs were regulated by 14 differentially expressed microRNAs(DEMIs)in 26 pairs.The candidate DEGs and DEMIs provide valuable insight for manipulating grain filling under different N rates.
