Evaluation of gamma interferon assay for detection of bovine tuberculosis in inactive foot and mouth disease vaccine administered cattle

Objective:To investigate the relationship between inactive foot and mouth disease virus (FMDV) vaccination and result of gamma interferon (IFN-γ) assay for tuberculosis in cattle negative for bovine tuberculosis. Methods:A total of 300 tuberculosis-negative cattles were subcutaneously vaccinated with inactive FMD vaccine. Nine hundred blood samples were collected from the same 300 cattles after 7, 15, and 30 days of vaccination. Then, all samples were tested with IFN-γ assay for tuberculosis. Results:A total of 900 blood samples were tested with IFN-γ assay for tuberculosis and all of the samples were found negative for bovine tuberculosis. Conclusions:Our results indicate that inactive FMD vaccination in cattle do not affect results of IFN-γ assay for tuberculosis.

Detection of Legionella pneumophila from domestic water and their antibiotic resistance profiles

Objective:To investigate the presence of Legionella pneumophila(L.pneumophila)in domestic water in Bitlis province and to determine the in vitro susceptibility of the isolates against several antibiotics.Methods:A total of 320 tap water samples were collected from the urban areas and villages of Bitlis province during the period from May to December 2010.All samples were cultured on plates of buffered charcoal yeast extract agar.L.pneumophila strains were tested for antimicrobial susceptibility by the disk diffusion method.Results:L.pneumophila strains were isolated from six(1.9%)domestic water samples.All isolates were typed as L.pneumophila serogroup 1 by latex agglutination test.Four of strains were isolated in July and two of them were detected in August.Antibiotic susceptibility testing was carried out on six L.pneumophila serogroup 1 isolates.Of the six strains,two was resistant to erythromycin and streptomycin,four were resistant to ampicillin and gentamicin,but all were sensitive to chloramphenicol and doxycycline.Conclusions:Our results indicate that L.pneumophila serogroup 1 is the most common type in the domestic water samples and threats public health.This is the first report of L.pneumophila in domestic water samples from Bitlis province.