CircMAN1A2 promotes vasculogenic mimicry of nasopharyngeal carcinoma cells through upregulating ERBB2 via sponging miR-940

Nasopharyngeal carcinoma(NPC)is the most prevalent human primary malignancy of the head and neck,and the presence of vasculogenic mimicry(VM)renders anti-angiogenic therapy ineffective and poorly prognostic.However,the underlying mechanisms are unclear.In the present study,we used miR-940 silencing and overexpression for in vitro NPC cell EdU staining,wound healing assay and 3D cell culture assay,and in vivo xenograft mouse model and VM formation to assess miR-940 function.We found that ectopic miR-940 expression reduced NPC cell proliferation,migration and VM,as well as tumorigenesis in vivo.By bioinformatic analysis,circMAN1A2 was identified as a circRNA that binds to miR-940.Mechanistically,we confirmed that circMAN1A2 acts as a sponge for miR-940,impairs the inhibitory effect of miR-940 on target ERBB2,and then activates the PI3K/AKT/mTOR signaling pathway using RNA-FISH,dual luciferase reporter gene and rescue analysis assays.In addition,upregulation of ERBB2 expression is associated with clinical staging and poor prognosis of NPC.Taken together,the present findings suggest that circMAN1A2 promotes VM formation and progression of NPC through miR-940/ERBB2 axis and further activates the PI3K/AKT/mTOR pathway.Therefore,circMAN1A2 may become a biomarker and therapeutic target for anti-angiogenic therapy in patients with nasopharyngeal carcinoma.

Hot Spot Analysis and Development Trend of Intelligent Roof Parking Lot:Bibliometric Analysis Based on CiteSpace Visualization Software

Using CiteSpace visualization software,literature research on roof parking lot in recent 20 years is analyzed,and the research direction and development of the relevant papers are integrated and induced.From utilization development,roof greening and technical practice,overall consideration and grasp of the feasibility of roof parking lot are conducted.Realization paths of two kinds of roof parking lots are provided:suspended PRT stereo parking lot and telescopic rail elevator,and it is committed to providing feasible suggestions and reference for future roof parking lot.

Stress-deconcentrated ultrasensitive strain sensor with hydrogen-bonding-tuned fracture resilience for robust biomechanical monitoring

Recently,rapid advances in flexible strain sensors have broadened their application scenario in monitoring of various mechanophysiological signals.Among various strain sensors,the crack-based strain sensors have drawn increasing attention in monitoring subtle mechanical deformation due to their high sensitivity.However,early generation and rapid propagation of cracks in the conductive sensing layer result in a narrow working range,limiting their application in monitoring large biomechanical signals.Herein,we developed a stress-deconcentrated ultrasensitive strain(SDUS)sensor with ultrahigh sensitivity(gauge factor up to2.3×10^(6))and a wide working range(0%-50%)via incorporating notch-insensitive elastic substrate and microcrack-tunable conductive layer.Furthermore,the highly elastic amine-based polymer-modified polydimethylsiloxane substrate without obvious hysteresis endows our SDUS sensor with a rapid response time(2.33 ms)to external stimuli.The accurate detection of the radial pulse,joint motion,and vocal cord vibration proves the capability of SDUS sensor for healthcare monitoring and human-machine communications.