目的探讨健脾化瘀解毒方对慢性萎缩性胃炎(CAG)大鼠增殖、分化ERK/Cyclin E信号通路的影响。方法选用SPF级SD大鼠,运用N-甲基-N’-硝基-N-亚硝基胍(MNNG)复合造模法复制CAG大鼠模型,设立分组:正常组,模型组,叶酸组,健脾化瘀解毒方高、...目的探讨健脾化瘀解毒方对慢性萎缩性胃炎(CAG)大鼠增殖、分化ERK/Cyclin E信号通路的影响。方法选用SPF级SD大鼠,运用N-甲基-N’-硝基-N-亚硝基胍(MNNG)复合造模法复制CAG大鼠模型,设立分组:正常组,模型组,叶酸组,健脾化瘀解毒方高、中、低组。HE染色观察大鼠胃黏膜组织病理学改变;采用qRT-PCR法检测胃黏膜组织ERK1、ERK2、Cyclin E mRNA转录水平;免疫组化法检测胃黏膜组织ERK1、ERK2、Cyclin E蛋白表达。结果健脾化瘀解毒方可显著改善CAG大鼠胃黏膜上皮腺体结构、形态紊乱及细胞异型性等病理表现。模型组CAG大鼠胃黏膜上皮的ERK1、ERK2、Cyclin E mRNA水平及蛋白表达评分均较正常组显著增加(P<0.05或P<0.01);与模型组比较,健脾化瘀解毒方能显著下调CAG大鼠胃黏膜上皮的ERK1、Cyclin E mRNA水平及蛋白表达评分(P<0.05,P<0.01),以高剂量组疗效最佳;健脾化瘀解毒方对ERK2无显著调控作用。结论健脾化瘀解毒方能显著下调ERK1、Cyclin E的表达,抑制胃黏膜上皮细胞的过度增殖,进而阻断CAG恶性转变。展开更多
Radical mastectomy may lead to suppression of cellular immune function in patients with malignant tumors,and affect the quality of life(QOL)of patients.Immune suppression is a common cause of complications and adverse...Radical mastectomy may lead to suppression of cellular immune function in patients with malignant tumors,and affect the quality of life(QOL)of patients.Immune suppression is a common cause of complications and adverse reactions in adjuvant therapy after radical mastectomy of breast cancer.Currently,there are few proven effective treatments for immune suppression.Therefore,it’s necessary to develop a new treatment method.Press needle is widely used in clinical practice.However,there have been relatively few studies that evaluate the effects of press needle on postoperative immune function.The aim of the present study is to assess the effects of press needle on immune function and QOL in female breast cancer patients undergoing radical mastectomy.This study will be a single-center,randomized and single-blinded trial.Totally 78 eligible patients will be randomized in a ratio of 1:1 to the press needle group or the sham press needle group.During the treatment phase,patients will undergo five times weekly of verum press needle or sham press needle for 2 weeks.The primary outcome measures will be the peripheral blood levels of CD8^(+),CD4^(+),CD3^(+),and CD4^(+)/CD8^(+)T cells.The secondary outcome measures will be the changes of patients’QOL,evaluated by the Karnofsky Performance Scale score and the EORTC core quality of life questionnaire(EORTC QLQ-C30).Furthermore,5-year survival rate and recurrence rate will be evaluated.Safety and adverse events will be assessed at each visit.The results of this on-going study will provide clinical evidence for the effects and safety of press needle on immune function and QOL in patients after breast cancer resection compared with sham press needle.展开更多
OBJECTIVE: To investigate the clinical differentiation of spleen-deficiency pattern(SDP), a group of symptoms and signs defined in terms of Traditional Chinese Medicine for its clinical practice.METHODS: Peripheral ve...OBJECTIVE: To investigate the clinical differentiation of spleen-deficiency pattern(SDP), a group of symptoms and signs defined in terms of Traditional Chinese Medicine for its clinical practice.METHODS: Peripheral venous blood(> 3 m L) was collected from each of six type 2 diabetes mellitus(T2DM)-SDP patients and six healthy volunteers. After the isolation of peripheral white blood cells(PWBCs), total RNA was extracted, and quality control was performed on all RNA samples. Microarray experiments were conducted using the Agilent human whole genome gene chip, and genes demonstrating differential expression were screened. Bioinformatics analysis was conducted on these genes using several online databases.RESULTS: We screened a total of 175 differentially expressed genes(DEGs), of which 111(63%) were down-regulated and 64(37%) were up-regulated in T2DM-SDP patients compared with healthy controls. Among the 175 genes, 158 had biological function annotations: 46(29%) were directly related to an individual's immune regulation or response, 25(16%) were associated with substance and energy metabolism of PWBCs which could also indirectly influence immunity, and the remaining87(55%) were involved in a variety of PWBC biological processes that might eventually influence the immune function. Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis revealed that the DEGs were predominantly enriched in seven immune-related pathways. Hierarchical cluster analysis identified gene expression patterns that were distinguishable between the two study groups.CONCLUSION: Our results suggest that T2DM-SDP patients experience significant hypoimmunity and/or immune dysfunctions, and possess a specific gene expression profile. These findings offer new insights into SDP and the clinical pattern differentiation of T2DM-SDP.展开更多
文摘目的探讨健脾化瘀解毒方对慢性萎缩性胃炎(CAG)大鼠增殖、分化ERK/Cyclin E信号通路的影响。方法选用SPF级SD大鼠,运用N-甲基-N’-硝基-N-亚硝基胍(MNNG)复合造模法复制CAG大鼠模型,设立分组:正常组,模型组,叶酸组,健脾化瘀解毒方高、中、低组。HE染色观察大鼠胃黏膜组织病理学改变;采用qRT-PCR法检测胃黏膜组织ERK1、ERK2、Cyclin E mRNA转录水平;免疫组化法检测胃黏膜组织ERK1、ERK2、Cyclin E蛋白表达。结果健脾化瘀解毒方可显著改善CAG大鼠胃黏膜上皮腺体结构、形态紊乱及细胞异型性等病理表现。模型组CAG大鼠胃黏膜上皮的ERK1、ERK2、Cyclin E mRNA水平及蛋白表达评分均较正常组显著增加(P<0.05或P<0.01);与模型组比较,健脾化瘀解毒方能显著下调CAG大鼠胃黏膜上皮的ERK1、Cyclin E mRNA水平及蛋白表达评分(P<0.05,P<0.01),以高剂量组疗效最佳;健脾化瘀解毒方对ERK2无显著调控作用。结论健脾化瘀解毒方能显著下调ERK1、Cyclin E的表达,抑制胃黏膜上皮细胞的过度增殖,进而阻断CAG恶性转变。
基金Supported by Study of Press Needle on Immune Function in Breast Cancer Patients after Radical Mastectomy(No.20ZL15)Enhancement of Scientific Research Capacity of“Hundred Talents Program”(No.20-Q17)。
文摘Radical mastectomy may lead to suppression of cellular immune function in patients with malignant tumors,and affect the quality of life(QOL)of patients.Immune suppression is a common cause of complications and adverse reactions in adjuvant therapy after radical mastectomy of breast cancer.Currently,there are few proven effective treatments for immune suppression.Therefore,it’s necessary to develop a new treatment method.Press needle is widely used in clinical practice.However,there have been relatively few studies that evaluate the effects of press needle on postoperative immune function.The aim of the present study is to assess the effects of press needle on immune function and QOL in female breast cancer patients undergoing radical mastectomy.This study will be a single-center,randomized and single-blinded trial.Totally 78 eligible patients will be randomized in a ratio of 1:1 to the press needle group or the sham press needle group.During the treatment phase,patients will undergo five times weekly of verum press needle or sham press needle for 2 weeks.The primary outcome measures will be the peripheral blood levels of CD8^(+),CD4^(+),CD3^(+),and CD4^(+)/CD8^(+)T cells.The secondary outcome measures will be the changes of patients’QOL,evaluated by the Karnofsky Performance Scale score and the EORTC core quality of life questionnaire(EORTC QLQ-C30).Furthermore,5-year survival rate and recurrence rate will be evaluated.Safety and adverse events will be assessed at each visit.The results of this on-going study will provide clinical evidence for the effects and safety of press needle on immune function and QOL in patients after breast cancer resection compared with sham press needle.
基金Supported by the Administration of Traditional Chinese Medicine of Guangdong Province of China(Study on the Relevance Between the Pi-Deficiency Syndrome and Gene Differential Expression Profile of Immunity and Metabolism in Type 2 Diabetic MellitusNo.20123001)+8 种基金Special Funds from the Central Finance of China in Support of the Development of Local Colleges and Universities[Collaborative Innovation Platform for the Prevention and Treatment of Significant and Refractory Pi-Wei DiseasesEducational Finance Grant No.338(2013)]the National Natural Science Foundation of China(the Mechanism Study of Salivary Alpha Amylase Activity Change in Pi-Deficiency Syndrome Patients Based on the AMY1 Copy Number VariationN-Glycosylated Protein Level and β-Adrenergic Receptor ActivationNo.81102703)the Science and Technology Planning Project of Guangdong Province of China (miRNA as Material Basis for the New Hypothesis"Pi-Metabolism Relevance"and Study on the Molecular Mechanisms of Treating Metabolic Disorders Through PiNo.2013A032500005)
文摘OBJECTIVE: To investigate the clinical differentiation of spleen-deficiency pattern(SDP), a group of symptoms and signs defined in terms of Traditional Chinese Medicine for its clinical practice.METHODS: Peripheral venous blood(> 3 m L) was collected from each of six type 2 diabetes mellitus(T2DM)-SDP patients and six healthy volunteers. After the isolation of peripheral white blood cells(PWBCs), total RNA was extracted, and quality control was performed on all RNA samples. Microarray experiments were conducted using the Agilent human whole genome gene chip, and genes demonstrating differential expression were screened. Bioinformatics analysis was conducted on these genes using several online databases.RESULTS: We screened a total of 175 differentially expressed genes(DEGs), of which 111(63%) were down-regulated and 64(37%) were up-regulated in T2DM-SDP patients compared with healthy controls. Among the 175 genes, 158 had biological function annotations: 46(29%) were directly related to an individual's immune regulation or response, 25(16%) were associated with substance and energy metabolism of PWBCs which could also indirectly influence immunity, and the remaining87(55%) were involved in a variety of PWBC biological processes that might eventually influence the immune function. Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis revealed that the DEGs were predominantly enriched in seven immune-related pathways. Hierarchical cluster analysis identified gene expression patterns that were distinguishable between the two study groups.CONCLUSION: Our results suggest that T2DM-SDP patients experience significant hypoimmunity and/or immune dysfunctions, and possess a specific gene expression profile. These findings offer new insights into SDP and the clinical pattern differentiation of T2DM-SDP.
