Spontaneously blinking probe, which switches between dark and bright state without UV or external additives, is extremely attractive in super resolution imaging of live cells. Herein, a clickable rhodamine spirolactam...Spontaneously blinking probe, which switches between dark and bright state without UV or external additives, is extremely attractive in super resolution imaging of live cells. Herein, a clickable rhodamine spirolactam probe, Atto565-Tet, is rationally constructed for spontaneously blinking after biorthogonal labelling and successfully applied to super resolution imaging of mitochondria and lysosomes.展开更多
基金supported by the National Natural Science Foundation of China (Nos. 21421005, 21576040, 21776037, 22004011)China Postdoctoral Science Foundation (Nos. BX20200073 and 2020M670754)Dalian Science and Technology Innovation Fund (No. 2020JJ25CY014)。
文摘Spontaneously blinking probe, which switches between dark and bright state without UV or external additives, is extremely attractive in super resolution imaging of live cells. Herein, a clickable rhodamine spirolactam probe, Atto565-Tet, is rationally constructed for spontaneously blinking after biorthogonal labelling and successfully applied to super resolution imaging of mitochondria and lysosomes.
