Background Lymphocyte function and homeostasis is associated with immune defence to infection.Apoptosis of lymphocytes might be a considerably important component which has an impact on immunity to infections in peopl...Background Lymphocyte function and homeostasis is associated with immune defence to infection.Apoptosis of lymphocytes might be a considerably important component which has an impact on immunity to infections in people with hyperglycemia.The aim of this study was to explore the mitochondrial apoptosis pathway of lymphocyte in diabetic patients.Methods Sixty patients with type 2 diabetes mellitus and fifty healthy volunteers were included in this study.Annexin V and propidiumiodide （Pl） were joined in the isolated lymphocytes and the rate of lymphocyte apoptosis was calculated with flow cytometry.Observation of the lymphocytes was done using transmission electron microscopy; mitochondria had been extracted and then mitochondrial membrane potential （MMP） was detected to assess mitochondrial function; the mRNA level of Bcl-2,cytochrome c （Cyt-C）,caspase-9 and caspase-3 were analyzed by real-time reverse transcriptionpolymerase chain reaction （RT-PCR）.Results Apoptosis rate of lymphocyte was significantly higher in diabetic group than that in normal control group （P ＜0.05）.Transmission electron microscopy showed lymphocyte shrinkage and breakage,chromatin condensation and less mitochondria; a fall in MMP levels was also evident; Bcl-2 concentration was reduced and the expressions of caspase-9,caspase-3 and Cyt-C were elevated （P ＜0.05） in diabetic patients.Conclusions The rate of lymphocyte apoptosis was significantly higher in type 2 diabetic patients than that in normal population.Mitochondrial apoptosis pathway may play a very important role in decreasing function of lymphocyte in diabetes.展开更多
基金This study was supported by grants from the National Natural Science Foundation of China （Nos. 81070677 and 81270885）, the National Basic Research Program of China （973 Program, No. 2012CB524900） and Science and Technology Commission of Shanghai Municipality （No. 10JC 1409000）.Acknowledgements： We thank Miss Simkute Vesta from Division of Diabetes, Endocrinology and Metabolism of Imperial College London. We would like to thank our chief nurse Zhu Chunfang who gave us a lot of help in specimen collection.
文摘Background Lymphocyte function and homeostasis is associated with immune defence to infection.Apoptosis of lymphocytes might be a considerably important component which has an impact on immunity to infections in people with hyperglycemia.The aim of this study was to explore the mitochondrial apoptosis pathway of lymphocyte in diabetic patients.Methods Sixty patients with type 2 diabetes mellitus and fifty healthy volunteers were included in this study.Annexin V and propidiumiodide （Pl） were joined in the isolated lymphocytes and the rate of lymphocyte apoptosis was calculated with flow cytometry.Observation of the lymphocytes was done using transmission electron microscopy; mitochondria had been extracted and then mitochondrial membrane potential （MMP） was detected to assess mitochondrial function; the mRNA level of Bcl-2,cytochrome c （Cyt-C）,caspase-9 and caspase-3 were analyzed by real-time reverse transcriptionpolymerase chain reaction （RT-PCR）.Results Apoptosis rate of lymphocyte was significantly higher in diabetic group than that in normal control group （P ＜0.05）.Transmission electron microscopy showed lymphocyte shrinkage and breakage,chromatin condensation and less mitochondria; a fall in MMP levels was also evident; Bcl-2 concentration was reduced and the expressions of caspase-9,caspase-3 and Cyt-C were elevated （P ＜0.05） in diabetic patients.Conclusions The rate of lymphocyte apoptosis was significantly higher in type 2 diabetic patients than that in normal population.Mitochondrial apoptosis pathway may play a very important role in decreasing function of lymphocyte in diabetes.
