目的分析2017-2018年湖南省脊灰实验室所用细胞系的滴度值、急性弛缓性麻痹(acute flaccid paralysis,AFP)病例病原学特征、非脊髓灰质炎肠道病毒(non-polio enterovirus,NPEV)分离率及分布情况,为维持无脊灰状态及预防其传播提供依据...目的分析2017-2018年湖南省脊灰实验室所用细胞系的滴度值、急性弛缓性麻痹(acute flaccid paralysis,AFP)病例病原学特征、非脊髓灰质炎肠道病毒(non-polio enterovirus,NPEV)分离率及分布情况,为维持无脊灰状态及预防其传播提供依据。方法依照WHO《脊髓灰质炎病毒检验手册》(2004年第四版)方法,对AFP病例粪便标本采用鼠肺细胞(mouse L cells expressing the human poliovirus receptor,L20B)和人横纹肌肉瘤细胞(rhabdomyosarcoma,RD)进行肠道病毒(enterovirus,EV)分离,L20B细胞阳性分离物转RD细胞阳性者再进行脊髓灰质炎型内鉴定实验,脊髓灰质炎阳性毒株送中国疾病预防控制中心国家脊髓灰质炎实验室进行型内鉴定。结果在506例(1011份)AFP病例标本中,分离出脊髓灰质炎病毒(polioviru,PV)2株、NPEV 77株,分离率分别为0.20%、7.62%;2株I型PV阳性毒株均有2个核苷酸序列变异,未发现脊髓灰质炎野病毒和疫苗衍生脊灰病毒,0~1岁组AFP病例NPEV分离率最高。结论2017-2018年湖南省脊灰实验室所用细胞系对脊灰病毒的敏感性良好,未发现脊灰野病毒,继续维持了无脊灰状态。展开更多
Mammalian target of rapamycin(mTOR),a serine/threonine kinase orchestrating cellular metabolism,is a crucial immune system regulator.However,it remains unclear how mTOR regulates dendritic cell(DC) function in vivo,es...Mammalian target of rapamycin(mTOR),a serine/threonine kinase orchestrating cellular metabolism,is a crucial immune system regulator.However,it remains unclear how mTOR regulates dendritic cell(DC) function in vivo,especially DC-T cell encounters,a critical step for initiating adaptive immune responses.We dynamically visualized DC-T contacts in mouse lymph node using confocal microscopy and established an encounter model to characterize the effect of mTOR inhibition on DC-T cell encounters using DC morphology.Quantitative data showed mTOR inhibition via rapamycin altered DC shape,with an increased form factor(30.17%) and decreased cellular surface area(20.36%) and perimeter(22.43%),which were associated with Cdc42 protein downregulation(52.71%).Additionally,DCs adopted a similar morphological change with Cdc42 inhibition via ZCL278 as that observed with mTOR inhibition.These morphologically altered DCs displayed low encounter rates with T cells.Time-lapse imaging data of T cell motility supported the simulated result of the encounter model,where antigen-specific T cells appeared to reduce arrest in the lymph nodes of rapamycin-pretreated mice relative to the untreated group.Therefore,mTOR inhibition altered DC morphology in vivo and decreased the DC-T cell encounter rate,as well as Cdc42 inhibition.By establishing an encounter model,our study provides an intuitive approach for the early prediction of DC function through morphological quantification of form factor and area.展开更多
文摘目的分析2017-2018年湖南省脊灰实验室所用细胞系的滴度值、急性弛缓性麻痹(acute flaccid paralysis,AFP)病例病原学特征、非脊髓灰质炎肠道病毒(non-polio enterovirus,NPEV)分离率及分布情况,为维持无脊灰状态及预防其传播提供依据。方法依照WHO《脊髓灰质炎病毒检验手册》(2004年第四版)方法,对AFP病例粪便标本采用鼠肺细胞(mouse L cells expressing the human poliovirus receptor,L20B)和人横纹肌肉瘤细胞(rhabdomyosarcoma,RD)进行肠道病毒(enterovirus,EV)分离,L20B细胞阳性分离物转RD细胞阳性者再进行脊髓灰质炎型内鉴定实验,脊髓灰质炎阳性毒株送中国疾病预防控制中心国家脊髓灰质炎实验室进行型内鉴定。结果在506例(1011份)AFP病例标本中,分离出脊髓灰质炎病毒(polioviru,PV)2株、NPEV 77株,分离率分别为0.20%、7.62%;2株I型PV阳性毒株均有2个核苷酸序列变异,未发现脊髓灰质炎野病毒和疫苗衍生脊灰病毒,0~1岁组AFP病例NPEV分离率最高。结论2017-2018年湖南省脊灰实验室所用细胞系对脊灰病毒的敏感性良好,未发现脊灰野病毒,继续维持了无脊灰状态。
基金supported by the Science Fund for Creative Research Groups of the National Natural Science Foundation of China (61721092)the Major Research Plan of the National Natural Science Foundation of China (91542000, 91442201)+2 种基金National Science Fund for Distinguished Young Scholars (81625012)National Natural Science Foundation of China (81501593)the Director Fund of WNLO
文摘Mammalian target of rapamycin(mTOR),a serine/threonine kinase orchestrating cellular metabolism,is a crucial immune system regulator.However,it remains unclear how mTOR regulates dendritic cell(DC) function in vivo,especially DC-T cell encounters,a critical step for initiating adaptive immune responses.We dynamically visualized DC-T contacts in mouse lymph node using confocal microscopy and established an encounter model to characterize the effect of mTOR inhibition on DC-T cell encounters using DC morphology.Quantitative data showed mTOR inhibition via rapamycin altered DC shape,with an increased form factor(30.17%) and decreased cellular surface area(20.36%) and perimeter(22.43%),which were associated with Cdc42 protein downregulation(52.71%).Additionally,DCs adopted a similar morphological change with Cdc42 inhibition via ZCL278 as that observed with mTOR inhibition.These morphologically altered DCs displayed low encounter rates with T cells.Time-lapse imaging data of T cell motility supported the simulated result of the encounter model,where antigen-specific T cells appeared to reduce arrest in the lymph nodes of rapamycin-pretreated mice relative to the untreated group.Therefore,mTOR inhibition altered DC morphology in vivo and decreased the DC-T cell encounter rate,as well as Cdc42 inhibition.By establishing an encounter model,our study provides an intuitive approach for the early prediction of DC function through morphological quantification of form factor and area.
