[Objectives] To optimize the extraction process and determine the total triterpenoids from Semen Trichosanthis.[Methods]The Box-behnken response surface methodology was applied to optimize the extraction conditions of...[Objectives] To optimize the extraction process and determine the total triterpenoids from Semen Trichosanthis.[Methods]The Box-behnken response surface methodology was applied to optimize the extraction conditions of total triterpenoids in Semen Trichosanthis.And the spectrophotometry was used to determine the content of total triterpenoids in Semen Trichosanthis.[Results] The optimal extraction conditions were solid to liquid ratio of 40∶ 1,ultrasonic time of 20 min,and ultrasonic power of 100 W.Under these conditions,the content of total triterpenoids in Semen Trichosanthis was significantly different among the 10 production areas,among which the No.9 production area(Anhui 1) had the highest total triterpenoids content of 45.71 mg/g,while No.7 production area(Shandong) had the lowest total triterpenoids content of 15.22 mg/g.[Conclusions]The Box-behnken response surface methodology is reliable for the extraction of total triterpenoids in Semen Trichosanthis,and the spectrophotometry is proper for determining the content of total triterpenoids in Semen Trichosanthis.There are large differences in the content of total triterpenoids in Semen Trichosanthis produced in different areas.展开更多
Sulfuric acid-phenol and sulfuric acid-anthrone methods were used to detect polysaccharide content in shoots of Aralia elata( Miq.) Seem.,and the conversion factor to glucose was measured with refined polysaccharide...Sulfuric acid-phenol and sulfuric acid-anthrone methods were used to detect polysaccharide content in shoots of Aralia elata( Miq.) Seem.,and the conversion factor to glucose was measured with refined polysaccharides. Comprehensive evaluation was carried out by linear relationship,precision,reproducibility,stability and recovery rate. The results showed that the linear relationship between glucose concentration and absorbance was good when glucose concentration was0-40 μg/ml,and the average recovery rate was equal to or higher than 97. 00% with good reproducibility( RSD 〈 1. 60%,n = 5). It revealed that the two methods were accurate and reliable,and suitable for the determination of polysaccharide content in the shoots of A. elata. Polysaccharide content detected by sulfuric acid-phenol and sulfuric acid-anthrone methods was 19. 31% and 20. 40% respectively.展开更多
[Objectives]The purpose of this study was to establish a method for the determination of five kinds of flavonoids in trichosanthes peel.[Methods]The contents of rutin,isoquercitrin,quercitrin,quercetin and kaempferol ...[Objectives]The purpose of this study was to establish a method for the determination of five kinds of flavonoids in trichosanthes peel.[Methods]The contents of rutin,isoquercitrin,quercitrin,quercetin and kaempferol in trichosanthes peel samples collected from different sites were determined by high-performance liquid chromatography.With each component as an index,principal component analysis and cluster analysis were performed.[Results]The contents of flavonoids in the trichosanthes peel samples from different sites were different.The cumulative variance contribution rate of isoquercitrin was 95.514%.The results of cluster analysis show that the quality of trichosanthes peel from Anhui was the best.[Conclusions]Taking isoquercitrin as a common factor to carry out content determination will help to better control the quality of trichosanthes peel.展开更多
基金Supported by Project of Department of Education of Liaoning Province(L2017lkyfwdf-05)Natural Science Foundation Project of Department of Education of Liaoning Province(20180551223&2016003003)
文摘[Objectives] To optimize the extraction process and determine the total triterpenoids from Semen Trichosanthis.[Methods]The Box-behnken response surface methodology was applied to optimize the extraction conditions of total triterpenoids in Semen Trichosanthis.And the spectrophotometry was used to determine the content of total triterpenoids in Semen Trichosanthis.[Results] The optimal extraction conditions were solid to liquid ratio of 40∶ 1,ultrasonic time of 20 min,and ultrasonic power of 100 W.Under these conditions,the content of total triterpenoids in Semen Trichosanthis was significantly different among the 10 production areas,among which the No.9 production area(Anhui 1) had the highest total triterpenoids content of 45.71 mg/g,while No.7 production area(Shandong) had the lowest total triterpenoids content of 15.22 mg/g.[Conclusions]The Box-behnken response surface methodology is reliable for the extraction of total triterpenoids in Semen Trichosanthis,and the spectrophotometry is proper for determining the content of total triterpenoids in Semen Trichosanthis.There are large differences in the content of total triterpenoids in Semen Trichosanthis produced in different areas.
基金Supported by Scientific Research Project of Education Department of Liaoning Province,China(L2017lkyfwdf-05)Public Welfare Fund Project of Department of Science and Technology of Liaoning Province,China(2016003003)
文摘Sulfuric acid-phenol and sulfuric acid-anthrone methods were used to detect polysaccharide content in shoots of Aralia elata( Miq.) Seem.,and the conversion factor to glucose was measured with refined polysaccharides. Comprehensive evaluation was carried out by linear relationship,precision,reproducibility,stability and recovery rate. The results showed that the linear relationship between glucose concentration and absorbance was good when glucose concentration was0-40 μg/ml,and the average recovery rate was equal to or higher than 97. 00% with good reproducibility( RSD 〈 1. 60%,n = 5). It revealed that the two methods were accurate and reliable,and suitable for the determination of polysaccharide content in the shoots of A. elata. Polysaccharide content detected by sulfuric acid-phenol and sulfuric acid-anthrone methods was 19. 31% and 20. 40% respectively.
基金College Students'Innovation and Entrepreneurship Training Program of Liaoning Province(201911430039).
文摘[Objectives]The purpose of this study was to establish a method for the determination of five kinds of flavonoids in trichosanthes peel.[Methods]The contents of rutin,isoquercitrin,quercitrin,quercetin and kaempferol in trichosanthes peel samples collected from different sites were determined by high-performance liquid chromatography.With each component as an index,principal component analysis and cluster analysis were performed.[Results]The contents of flavonoids in the trichosanthes peel samples from different sites were different.The cumulative variance contribution rate of isoquercitrin was 95.514%.The results of cluster analysis show that the quality of trichosanthes peel from Anhui was the best.[Conclusions]Taking isoquercitrin as a common factor to carry out content determination will help to better control the quality of trichosanthes peel.