冰川作为地球主要的冰冻圈要素之一,蕴藏着丰富的微生物资源。本文通过培养和16S r RNA基因序列鉴定,研究了新疆北部木斯岛冰川的表层雪、表层冰、深层冰、表层融水和前沿土壤生境中的可培养细菌群落结构。结果表明,从木斯岛冰川中培养...冰川作为地球主要的冰冻圈要素之一,蕴藏着丰富的微生物资源。本文通过培养和16S r RNA基因序列鉴定,研究了新疆北部木斯岛冰川的表层雪、表层冰、深层冰、表层融水和前沿土壤生境中的可培养细菌群落结构。结果表明,从木斯岛冰川中培养的细菌属于拟杆菌门(Bacteroidetes)、变形菌门(Proteobacteria)、奇异球菌-栖热菌门(Deinococcus-Thermus)和放线菌门(Actinobacteria),优势门为变形菌门和放线菌门,占所有分离菌株数量的78%。优势属为黄杆菌属(Flavobacterium)、假单胞菌属(Pseudomonas)和冷杆菌属(Cryobacterium),有15株细菌为潜在新种。在10℃和25℃下培养的菌落数分别为6.0×10^(1)~5.3×10^(3)CFU·m L^(-1)(CFU·g^(-1))和3.1×10^(1)~5.1×10^(3)CFU·m L^(-1)(CFU·g^(-1))。木斯岛冰川不同生境物种多样性差异较大,前沿土壤生境物种多样性最高,表层融水生境物种多样性最低。可培养细菌具有生境特异性,表层雪、表层冰、深层冰和前沿土壤生境分别有2、2、1和19个特有的属,假单胞菌属是五种生境的共有属,许多源自雪的细菌类群可以在不同生境中保存下来。在10℃和25℃下共培养出16个共有属,分别有9个和7个特有属。木斯岛冰川蕴含丰富的菌株多样性,可为开发冰川微生物资源提供数据支持和菌株资源。展开更多
转脂蛋白(Lipid transfer protein,LTP)广泛参与植物的各种生长发育进程及逆境响应,但对其表达调控规律的研究则鲜有报道。首先鉴定了ZmLTP3基因上游启动子的核心序列,进而构建了玉米(Zea mays L.)自交系B73的cDNA表达文库,克隆了ZmLTP...转脂蛋白(Lipid transfer protein,LTP)广泛参与植物的各种生长发育进程及逆境响应,但对其表达调控规律的研究则鲜有报道。首先鉴定了ZmLTP3基因上游启动子的核心序列,进而构建了玉米(Zea mays L.)自交系B73的cDNA表达文库,克隆了ZmLTP3基因上游启动子区域,利用酵母单杂交技术筛选,筛选ZmLTP3基因启动子序列互作蛋白。通过测序和生物信息学分析,共筛选到13个互作蛋白cDNA。其中ZmLSD1具有典型的转录因子特征,属于C4类型锌指蛋白家族成员。EMSA检测结果表明,只有ZmLSD1蛋白可以与来自ZmLTP3基因启动子的DNA探针序列结合。上述结果表明ZmLSD1能与ZmLTP3基因启动子结合,并调控其表达的转录因子。展开更多
The Loess Plateau,located in Gansu Province,is an important energy base in China because most of the oil and gas resources are distributed in Gansu Province.In the last 40 a,ecological environment in this region has b...The Loess Plateau,located in Gansu Province,is an important energy base in China because most of the oil and gas resources are distributed in Gansu Province.In the last 40 a,ecological environment in this region has been extremely destroyed due to the over-exploitation of crude-oil resources.Remediation of crude-oil contaminated soil in this area remains to be a challenging task.In this study,in order to elucidate the effects of organic compost and biochar on phytoremediation of crude-oil contaminated soil(20 g/kg)by Calendula officinalis,we designed five treatments,i.e.,natural attenuation(CK),planted C.officinalis only(P),planted C.officinalis with biochar amendment(PB),planted C.officinalis with organic compost amendment(PC),and planted C.officinalis with co-amendment of biochar and organic compost(PBC).After 152 d of cultivation,total petroleum hydrocarbons(TPH)removal rates of CK,P,PB,PC and PBC were 6.36%,50.08%,39.58%,73.10%and 59.87%,respectively.Shoot and root dry weights of C.officinalis significantly increased by 172.31%and 80.96%under PC and 311.61%and 145.43%under PBC,respectively as compared with P(P<0.05).Total chlorophyll contents in leaves of C.officinalis under P,PC and PBC significantly increased by 77.36%,125.50%and 79.80%,respectively(P<0.05)as compared with PB.Physical-chemical characteristics and enzymatic activity of soil in different treatments were also assessed.The highest total N,total P,available N,available P and SOM(soil organic matter)occurred in PC,followed by PBC(P<0.05).C.officinalis rhizospheric soil dehydrogenase(DHA)and polyphenol oxidase(PPO)activities in PB were lower than those of other treatments(P<0.05).The values of ACE(abundance-based coverage estimators)and Chao 1 indices for rhizospheric bacteria were the highest under PC followed by PBC,P,PB and CK(P<0.05).However,the Shannon index for bacteria was the highest under PC and PBC,followed by P,PB and CK(P<0.05).In terms of soil microbial community composition,Proteiniphilum,Immundisolibacteraceae and Solimonadaceae were relatively more abundant under PC and PBC.Relative abundances of Pseudallescheria,Ochroconis,Fusarium,Sarocladium,Podospora,Apodus,Pyrenochaetopsis and Schizothecium under PC and PBC were higher,while relative abundances of Gliomastix,Aspergillus and Alternaria were lower under PC and PBC.As per the nonmetric multidimensional scaling(NMDS)analysis,application of organic compost significantly promoted soil N and P contents,shoot length,root vitality,chlorophyll ratio,total chlorophyll,abundance and diversity of rhizospheric soil microbial community in C.officinalis.A high p H value and lower soil N and P contents induced by biochar,altered C.officinalis rhizospheric soil microbial community composition,which might have restrained its phytoremediation efficiency.The results suggest that organic compost-assisted C.officinalis phytoremediation for crude-oil contaminated soil was highly effective in the Loess Plateau,China.展开更多
Blue-green electroluminescence has been observed in free-standing diamond films which were deposited by microwave plasma assisted CVD on silicon substrates.The electroluminescence device is driven by a 60 Hz power sup...Blue-green electroluminescence has been observed in free-standing diamond films which were deposited by microwave plasma assisted CVD on silicon substrates.The electroluminescence device is driven by a 60 Hz power supply.The threshold voltage was about 112 V peak-to-peak.The electroluminescence spectrum at room temperature,showed a blue-green band with the peak centered at 485nm suggesting band A type emission.Electroluminescence was also observed at 77K.展开更多
A kind of highly transparent A1_(2)O_(3)ceramics is fabricated in a way of reducing the scattering origins such as the boundary scattering and the facies-difference scattering.The ceramics is a hopeful substitute of t...A kind of highly transparent A1_(2)O_(3)ceramics is fabricated in a way of reducing the scattering origins such as the boundary scattering and the facies-difference scattering.The ceramics is a hopeful substitute of the optoelectronic detector windows made of crystalline A1_(2)O_(3)with several advantages such as lower cost,improvement of detecting accuracy and increase of the damage threshold etc.展开更多
文摘冰川作为地球主要的冰冻圈要素之一,蕴藏着丰富的微生物资源。本文通过培养和16S r RNA基因序列鉴定,研究了新疆北部木斯岛冰川的表层雪、表层冰、深层冰、表层融水和前沿土壤生境中的可培养细菌群落结构。结果表明,从木斯岛冰川中培养的细菌属于拟杆菌门(Bacteroidetes)、变形菌门(Proteobacteria)、奇异球菌-栖热菌门(Deinococcus-Thermus)和放线菌门(Actinobacteria),优势门为变形菌门和放线菌门,占所有分离菌株数量的78%。优势属为黄杆菌属(Flavobacterium)、假单胞菌属(Pseudomonas)和冷杆菌属(Cryobacterium),有15株细菌为潜在新种。在10℃和25℃下培养的菌落数分别为6.0×10^(1)~5.3×10^(3)CFU·m L^(-1)(CFU·g^(-1))和3.1×10^(1)~5.1×10^(3)CFU·m L^(-1)(CFU·g^(-1))。木斯岛冰川不同生境物种多样性差异较大,前沿土壤生境物种多样性最高,表层融水生境物种多样性最低。可培养细菌具有生境特异性,表层雪、表层冰、深层冰和前沿土壤生境分别有2、2、1和19个特有的属,假单胞菌属是五种生境的共有属,许多源自雪的细菌类群可以在不同生境中保存下来。在10℃和25℃下共培养出16个共有属,分别有9个和7个特有属。木斯岛冰川蕴含丰富的菌株多样性,可为开发冰川微生物资源提供数据支持和菌株资源。
文摘转脂蛋白(Lipid transfer protein,LTP)广泛参与植物的各种生长发育进程及逆境响应,但对其表达调控规律的研究则鲜有报道。首先鉴定了ZmLTP3基因上游启动子的核心序列,进而构建了玉米(Zea mays L.)自交系B73的cDNA表达文库,克隆了ZmLTP3基因上游启动子区域,利用酵母单杂交技术筛选,筛选ZmLTP3基因启动子序列互作蛋白。通过测序和生物信息学分析,共筛选到13个互作蛋白cDNA。其中ZmLSD1具有典型的转录因子特征,属于C4类型锌指蛋白家族成员。EMSA检测结果表明,只有ZmLSD1蛋白可以与来自ZmLTP3基因启动子的DNA探针序列结合。上述结果表明ZmLSD1能与ZmLTP3基因启动子结合,并调控其表达的转录因子。
基金funded by the Scientific Project of Gansu Province,China(20JR5RA548)the National Natural Science Foundation of China(31860148)+1 种基金the Natural Science Foundation of Gansu Province,China(21JR1RM333)the Doctoral Program of Longdong University,China(XYBY1909)。
文摘The Loess Plateau,located in Gansu Province,is an important energy base in China because most of the oil and gas resources are distributed in Gansu Province.In the last 40 a,ecological environment in this region has been extremely destroyed due to the over-exploitation of crude-oil resources.Remediation of crude-oil contaminated soil in this area remains to be a challenging task.In this study,in order to elucidate the effects of organic compost and biochar on phytoremediation of crude-oil contaminated soil(20 g/kg)by Calendula officinalis,we designed five treatments,i.e.,natural attenuation(CK),planted C.officinalis only(P),planted C.officinalis with biochar amendment(PB),planted C.officinalis with organic compost amendment(PC),and planted C.officinalis with co-amendment of biochar and organic compost(PBC).After 152 d of cultivation,total petroleum hydrocarbons(TPH)removal rates of CK,P,PB,PC and PBC were 6.36%,50.08%,39.58%,73.10%and 59.87%,respectively.Shoot and root dry weights of C.officinalis significantly increased by 172.31%and 80.96%under PC and 311.61%and 145.43%under PBC,respectively as compared with P(P<0.05).Total chlorophyll contents in leaves of C.officinalis under P,PC and PBC significantly increased by 77.36%,125.50%and 79.80%,respectively(P<0.05)as compared with PB.Physical-chemical characteristics and enzymatic activity of soil in different treatments were also assessed.The highest total N,total P,available N,available P and SOM(soil organic matter)occurred in PC,followed by PBC(P<0.05).C.officinalis rhizospheric soil dehydrogenase(DHA)and polyphenol oxidase(PPO)activities in PB were lower than those of other treatments(P<0.05).The values of ACE(abundance-based coverage estimators)and Chao 1 indices for rhizospheric bacteria were the highest under PC followed by PBC,P,PB and CK(P<0.05).However,the Shannon index for bacteria was the highest under PC and PBC,followed by P,PB and CK(P<0.05).In terms of soil microbial community composition,Proteiniphilum,Immundisolibacteraceae and Solimonadaceae were relatively more abundant under PC and PBC.Relative abundances of Pseudallescheria,Ochroconis,Fusarium,Sarocladium,Podospora,Apodus,Pyrenochaetopsis and Schizothecium under PC and PBC were higher,while relative abundances of Gliomastix,Aspergillus and Alternaria were lower under PC and PBC.As per the nonmetric multidimensional scaling(NMDS)analysis,application of organic compost significantly promoted soil N and P contents,shoot length,root vitality,chlorophyll ratio,total chlorophyll,abundance and diversity of rhizospheric soil microbial community in C.officinalis.A high p H value and lower soil N and P contents induced by biochar,altered C.officinalis rhizospheric soil microbial community composition,which might have restrained its phytoremediation efficiency.The results suggest that organic compost-assisted C.officinalis phytoremediation for crude-oil contaminated soil was highly effective in the Loess Plateau,China.
基金the National Natural Science Foundation of China,and the Natural Science Foundation of He'nan province.
文摘Blue-green electroluminescence has been observed in free-standing diamond films which were deposited by microwave plasma assisted CVD on silicon substrates.The electroluminescence device is driven by a 60 Hz power supply.The threshold voltage was about 112 V peak-to-peak.The electroluminescence spectrum at room temperature,showed a blue-green band with the peak centered at 485nm suggesting band A type emission.Electroluminescence was also observed at 77K.
文摘A kind of highly transparent A1_(2)O_(3)ceramics is fabricated in a way of reducing the scattering origins such as the boundary scattering and the facies-difference scattering.The ceramics is a hopeful substitute of the optoelectronic detector windows made of crystalline A1_(2)O_(3)with several advantages such as lower cost,improvement of detecting accuracy and increase of the damage threshold etc.
