目的探究循环肿瘤细胞在可切除食管鳞癌患者中的表达水平及其临床意义。方法选取36例经手术治疗的食管鳞癌患者,采用FR-CTC检测方法检测患者术前、术后外周静脉血中CTC水平,分析CTC水平与其他临床病理资料的关系,通过比较术前术后外周血...目的探究循环肿瘤细胞在可切除食管鳞癌患者中的表达水平及其临床意义。方法选取36例经手术治疗的食管鳞癌患者,采用FR-CTC检测方法检测患者术前、术后外周静脉血中CTC水平,分析CTC水平与其他临床病理资料的关系,通过比较术前术后外周血CTC水平的变化,探究手术对CTC水平的影响。结果本研究共入组36名患者,其中男性32人,女性4人,采集术前外周血标本36例,CTC检测结果 2.25～27.5 Unit/3 m L,采集术后标本19例,CTC检测结果 2.33～42.41 Unit/3 m L,接受三切口食管癌根治术的病人术前CTC水平显著低于两切口和左开胸病人(P=0.02),术后病理N0的患者其术前CTC水平显著高于病理N1-2的病人(P=0.01)。此外,对于无淋巴结转移的患者(23例),随着T分期的不断升高,CTC表达水平也相应升高(P=0.02)。选取了19例患者术前术后配对标本进行检测,术前术后患者CTC水平差异无统计学意义(P=0.29)。结论采用FR-CTC技术可有效检测食管鳞癌患者外周血CTC水平,术前CTC水平与肿瘤部位、病理T分期显著相关,有助于评估疾病进展程度,手术治疗并未导致肿瘤细胞进一步播散。展开更多
Nanoparticles with the anti-wear and friction reducing features were applied as cooling lubricant in the grinding fluid. Dry grinding, flood grinding, minimal quantity of lubrication （MQL）, and nanoparticle jet MQL ...Nanoparticles with the anti-wear and friction reducing features were applied as cooling lubricant in the grinding fluid. Dry grinding, flood grinding, minimal quantity of lubrication （MQL）, and nanoparticle jet MQL were used in the grinding experiments. The specific grinding energy of dry grinding, flood grinding and MQL were 84, 29.8, 45.5 J/mm3, respectively. The speci- fic grinding energy significantly decreased to 32.7 J/mm3 in nanoparticle MQL. Compared with dry grinding, the surface roughness values of flood grinding, MQL, and nanoparticle jet MQL were sig- nificantly reduced with the surface topography profile values reduced by 11%, 2.5%, and 10%, respectively, and the ten point height of microcosmic unflatness values reduced by 1.5%, 0.5%, and 1.3%, respectively. These results verified the satisfactory lubrication effects of nanoparticle MQL. MoS：, carbon nanotube （CNT）, and ZrO2 nanoparticles were also added in the grinding fluid of nanoparticle jet MQL to analyze their grinding surface lubrication effects. The specific grinding energy of MoS2 nanoparticle was only 32.7 J/mm3, which was 8.22% and 10.39% lower than those of the other two nanoparticles. Moreover, the surface roughness of workpiece was also smaller with MoS2 nanoparticle, which indicated its remarkable lubrication effects. Furthermore, the role of MoS2 particles in the grinding surface lubrication at different nanoparticle volume con- centrations was analyzed. MoS2 volume concentrations of 1%, 2%, and 3% were used. Experimental results revealed that the specific grinding energy and the workpiece surface roughness initially increased and then decreased as MoS2 nanoparticle volume concentration increased. Satisfactory grinding surface lubrication effects were obtained with 2% MoS2 nanoparticle volume concentration.展开更多
文摘目的探究循环肿瘤细胞在可切除食管鳞癌患者中的表达水平及其临床意义。方法选取36例经手术治疗的食管鳞癌患者,采用FR-CTC检测方法检测患者术前、术后外周静脉血中CTC水平,分析CTC水平与其他临床病理资料的关系,通过比较术前术后外周血CTC水平的变化,探究手术对CTC水平的影响。结果本研究共入组36名患者,其中男性32人,女性4人,采集术前外周血标本36例,CTC检测结果 2.25～27.5 Unit/3 m L,采集术后标本19例,CTC检测结果 2.33～42.41 Unit/3 m L,接受三切口食管癌根治术的病人术前CTC水平显著低于两切口和左开胸病人(P=0.02),术后病理N0的患者其术前CTC水平显著高于病理N1-2的病人(P=0.01)。此外,对于无淋巴结转移的患者(23例),随着T分期的不断升高,CTC表达水平也相应升高(P=0.02)。选取了19例患者术前术后配对标本进行检测,术前术后患者CTC水平差异无统计学意义(P=0.29)。结论采用FR-CTC技术可有效检测食管鳞癌患者外周血CTC水平,术前CTC水平与肿瘤部位、病理T分期显著相关,有助于评估疾病进展程度,手术治疗并未导致肿瘤细胞进一步播散。
基金co-supported by the National Natural Science Foundation of China (No. 51175276)the Qingdao Science and Technology Program of Basic Research Projects (No. 14-2-4-18-jch) of Chinathe Huangdao District Application Science and Technology Project (No. 2014-1-55) of China
文摘Nanoparticles with the anti-wear and friction reducing features were applied as cooling lubricant in the grinding fluid. Dry grinding, flood grinding, minimal quantity of lubrication （MQL）, and nanoparticle jet MQL were used in the grinding experiments. The specific grinding energy of dry grinding, flood grinding and MQL were 84, 29.8, 45.5 J/mm3, respectively. The speci- fic grinding energy significantly decreased to 32.7 J/mm3 in nanoparticle MQL. Compared with dry grinding, the surface roughness values of flood grinding, MQL, and nanoparticle jet MQL were sig- nificantly reduced with the surface topography profile values reduced by 11%, 2.5%, and 10%, respectively, and the ten point height of microcosmic unflatness values reduced by 1.5%, 0.5%, and 1.3%, respectively. These results verified the satisfactory lubrication effects of nanoparticle MQL. MoS：, carbon nanotube （CNT）, and ZrO2 nanoparticles were also added in the grinding fluid of nanoparticle jet MQL to analyze their grinding surface lubrication effects. The specific grinding energy of MoS2 nanoparticle was only 32.7 J/mm3, which was 8.22% and 10.39% lower than those of the other two nanoparticles. Moreover, the surface roughness of workpiece was also smaller with MoS2 nanoparticle, which indicated its remarkable lubrication effects. Furthermore, the role of MoS2 particles in the grinding surface lubrication at different nanoparticle volume con- centrations was analyzed. MoS2 volume concentrations of 1%, 2%, and 3% were used. Experimental results revealed that the specific grinding energy and the workpiece surface roughness initially increased and then decreased as MoS2 nanoparticle volume concentration increased. Satisfactory grinding surface lubrication effects were obtained with 2% MoS2 nanoparticle volume concentration.
