In recent years, the occurrence of rice false smut has become increasingly serious and the area of damage has expanded year by year, so the false smut has become one of the diseases which have seriously affected rice ...In recent years, the occurrence of rice false smut has become increasingly serious and the area of damage has expanded year by year, so the false smut has become one of the diseases which have seriously affected rice yield and quality in Heilongjiang Province. However, the genetic diversity research on false smut in Heilongjiang Province was relatively weak. In the current investigation, seven polymorphic SSR markers were taken to analyze the genetic diversity among 89 strains of the pathogens of rice false smut collected from five main rice growing areas in Heilongjiang Province, China. The results showed that the amplified bands of each pair of primers were between 2 and 7. A total of 43 bands were obtained, and each primer was expanded for 4.8 bands. The genetic similarity coefficient(GSC) between strains by using SSR molecular marker analysis showed that the GSC of the strain of Ustilaginoidea virens was 0.613-0.955, with an average of 0.741. According to the results cluster analysis, when the genetic distance was 0.73, 89 strains were divided into three groups, and five strains of rice smut strains were distributed in three populations, and strains in Harbin City were mainly distributed in group Ⅲ. In Mudanjiang City, the strains were mainly distributed in groups Ⅰ and Ⅲ, and the strains in Suihua City were mainly distributed in groups Ⅰ and Ⅱ, and the strains in Jiamusi City were evenly distributed in groups Ⅰ, Ⅱ and Ⅲ, and the strains in Qiqihar City were mainly distributed in groups Ⅱ and Ⅲ. The results showed that the genetic background of Ustilaginoidea virens in Heilongjiang Province was relatively low in complexity, and the overall inheritance of the strain was relatively stable.展开更多
Rice bacterial leaf brown spot disease caused by Pseudomonas syringae pv.syringae(Pss)is a major disease on rice.In recent years,Pss has emerged worldwide,seriously affecting rice production.It is very important to es...Rice bacterial leaf brown spot disease caused by Pseudomonas syringae pv.syringae(Pss)is a major disease on rice.In recent years,Pss has emerged worldwide,seriously affecting rice production.It is very important to establish a rapid detection method of Pss for the diagnosis and prevention of this disease.In order to robust and accurately diagnose the rice bacterial leaf brown spot disease in the field and laboratory,an assay system for the Pss was developed in this study,and the specific sequence of hrcN was used as the target,based on loop-mediated isothermal amplification(LAMP).The best detection system was MgSO 48 mmol·L^(-1),Bst DNA polymerase 8 U,dNTP 1.4 mmol·L^(-1),the ratio of internal and outer primers was 2:1,the reaction temperature was 63℃,the reaction time was 45 min,and the lowest sensitivity was 104 CFU·mL^(-1).This results provided an accurate and robust method for laboratory and field diagnosis of bacterial leaf brown spot disease of rice.展开更多
Rice false smut is a destructive disease that affects rice grain badly.The disease seriously affects the yield and quality of rice in Heilongjiang Province.In this paper,a pair of specific primers was designed to dete...Rice false smut is a destructive disease that affects rice grain badly.The disease seriously affects the yield and quality of rice in Heilongjiang Province.In this paper,a pair of specific primers was designed to detect the false smut pathogen rapidly and efficiently.The results showed that the pair of primers had strong specificity for false smut pathogen.In addition,the sensitivity of this primer to the genomic DNA of rice false smut pathogen in PCR reaction was 1 pg.By using these primers,the rice false smut pathogen could be detected within 48 h after inoculation,and a PCR reaction system with good specificity and high sensitivity was established.展开更多
目的:评价腹腔镜髂耻韧带悬吊术在盆腔器官脱垂中的临床效果及安全性。方法:回顾分析2019年1月至2021年12月为36例盆腔器官脱垂定量分度法(pelvic organ prolapse quantitation,POP-Q)≥Ⅱ度,以中盆腔脱垂为主的盆腔器官脱垂患者行腹腔...目的:评价腹腔镜髂耻韧带悬吊术在盆腔器官脱垂中的临床效果及安全性。方法:回顾分析2019年1月至2021年12月为36例盆腔器官脱垂定量分度法(pelvic organ prolapse quantitation,POP-Q)≥Ⅱ度,以中盆腔脱垂为主的盆腔器官脱垂患者行腹腔镜髂耻韧带悬吊术的临床资料,比较术前及术后6个月POP-Q评分、生活质量评分,以评估客观治愈率、主观满意度,并观察手术并发症情况。结果:36例均成功完成腹腔镜髂耻韧带悬吊术,其中28例保留子宫。手术时间平均(38.05±8.04)min,出血量平均(20.00±9.85)mL,术中无血管、肠管、输尿管、膀胱及神经损伤,术后住院(4.47±1.02)d,无术后出血、血肿发生,留置导尿24~48 h,其中1例术后发生尿潴留,治疗后排尿通畅。随访6~24个月,未发现阴道网片暴露,无盆腔疼痛、新发压力性尿失禁及排便困难。术后1年复发1例。与术前相比,术后6个月Aa、Ba、C、D、Ap、Bp点的POP-Q值得到纠正,差异有统计学意义(P<0.01)。手术前后盆底障碍影响简易问卷7评分[(82.86±7.56)vs.(23.79±9.36)]、盆底功能障碍问卷表简表20评分[(65.41±11.10)vs.(23.47±10.57)]、盆腔器官脱垂/尿失禁性功能问卷表12评分[(32.70±10.30)vs.(15.70±7.48)]差异有统计学意义(P<0.01)。结论:腹腔镜髂耻韧带悬吊术易于分离及定位锚定点,手术并发症少,容易学习,尤其利于在基层医院推广应用,为治疗盆腔器官脱垂提供了新的术式选择。展开更多
目的:对证据图研究进行文献计量学分析,以期了解目前证据图研究的现状并为学者开展相关研究提供借鉴和参考。方法:通过检索PubMed、EMBASE.com、Cochrane Library和 Web of Science获取证据图文献。利用VOSviewer 1.6.10软件对纳入研究...目的:对证据图研究进行文献计量学分析,以期了解目前证据图研究的现状并为学者开展相关研究提供借鉴和参考。方法:通过检索PubMed、EMBASE.com、Cochrane Library和 Web of Science获取证据图文献。利用VOSviewer 1.6.10软件对纳入研究的作者、国家、机构和关键词等信息进行提取,并对国家生成可视化合作网络图,对关键词进行聚类分析。采用CiteSpace V软件分析高产作者和高产机构之间的关系并生成可视化知识图谱。结果:共纳入证据图研究112篇,发文量最多的国家为美国(50篇),发文量最多的机构为塔夫茨大学(11篇),591位作者参与了证据图研究的发表,但发文量大于2篇的作者仅占4.74%。主要机构形成了3个研究团队,主要作者形成了6个合作团体,但各作者之间、机构之间合作较少。研究的主题主要有4个,主要的研究热点主题是肥胖、营养等人类的公共健康、婴幼儿的临床状况和青少年的心理健康。结论:高产的作者较少,参与研究的团体较多,各团体、机构之间的联系不够紧密,仍需进一步增强合作。证据图研究主题不多,仍需开拓新的研究主题。展开更多
基金Supported by the Natural Science Foundation of Heilongjiang Province(C2017032)Applied Technology Research and Development Program of Heilongjiang Province(GA19B104)。
文摘In recent years, the occurrence of rice false smut has become increasingly serious and the area of damage has expanded year by year, so the false smut has become one of the diseases which have seriously affected rice yield and quality in Heilongjiang Province. However, the genetic diversity research on false smut in Heilongjiang Province was relatively weak. In the current investigation, seven polymorphic SSR markers were taken to analyze the genetic diversity among 89 strains of the pathogens of rice false smut collected from five main rice growing areas in Heilongjiang Province, China. The results showed that the amplified bands of each pair of primers were between 2 and 7. A total of 43 bands were obtained, and each primer was expanded for 4.8 bands. The genetic similarity coefficient(GSC) between strains by using SSR molecular marker analysis showed that the GSC of the strain of Ustilaginoidea virens was 0.613-0.955, with an average of 0.741. According to the results cluster analysis, when the genetic distance was 0.73, 89 strains were divided into three groups, and five strains of rice smut strains were distributed in three populations, and strains in Harbin City were mainly distributed in group Ⅲ. In Mudanjiang City, the strains were mainly distributed in groups Ⅰ and Ⅲ, and the strains in Suihua City were mainly distributed in groups Ⅰ and Ⅱ, and the strains in Jiamusi City were evenly distributed in groups Ⅰ, Ⅱ and Ⅲ, and the strains in Qiqihar City were mainly distributed in groups Ⅱ and Ⅲ. The results showed that the genetic background of Ustilaginoidea virens in Heilongjiang Province was relatively low in complexity, and the overall inheritance of the strain was relatively stable.
基金Supported by the Natural Science Foundation of Heilongjiang Province(Topic C2017032)Heilongjiang Province Applied Technology Research and Development Program(Topic GA19B104)the National Key Research and Development Program(Topic 2018YFD0300105)。
文摘Rice bacterial leaf brown spot disease caused by Pseudomonas syringae pv.syringae(Pss)is a major disease on rice.In recent years,Pss has emerged worldwide,seriously affecting rice production.It is very important to establish a rapid detection method of Pss for the diagnosis and prevention of this disease.In order to robust and accurately diagnose the rice bacterial leaf brown spot disease in the field and laboratory,an assay system for the Pss was developed in this study,and the specific sequence of hrcN was used as the target,based on loop-mediated isothermal amplification(LAMP).The best detection system was MgSO 48 mmol·L^(-1),Bst DNA polymerase 8 U,dNTP 1.4 mmol·L^(-1),the ratio of internal and outer primers was 2:1,the reaction temperature was 63℃,the reaction time was 45 min,and the lowest sensitivity was 104 CFU·mL^(-1).This results provided an accurate and robust method for laboratory and field diagnosis of bacterial leaf brown spot disease of rice.
基金Supported by the Science and Technology Precision Poverty Alleviation Project of Planting Industry(ZY18C08)Special Project to Guide the Development of Central and Local Science and Technology。
文摘Rice false smut is a destructive disease that affects rice grain badly.The disease seriously affects the yield and quality of rice in Heilongjiang Province.In this paper,a pair of specific primers was designed to detect the false smut pathogen rapidly and efficiently.The results showed that the pair of primers had strong specificity for false smut pathogen.In addition,the sensitivity of this primer to the genomic DNA of rice false smut pathogen in PCR reaction was 1 pg.By using these primers,the rice false smut pathogen could be detected within 48 h after inoculation,and a PCR reaction system with good specificity and high sensitivity was established.
文摘目的:评价腹腔镜髂耻韧带悬吊术在盆腔器官脱垂中的临床效果及安全性。方法:回顾分析2019年1月至2021年12月为36例盆腔器官脱垂定量分度法(pelvic organ prolapse quantitation,POP-Q)≥Ⅱ度,以中盆腔脱垂为主的盆腔器官脱垂患者行腹腔镜髂耻韧带悬吊术的临床资料,比较术前及术后6个月POP-Q评分、生活质量评分,以评估客观治愈率、主观满意度,并观察手术并发症情况。结果:36例均成功完成腹腔镜髂耻韧带悬吊术,其中28例保留子宫。手术时间平均(38.05±8.04)min,出血量平均(20.00±9.85)mL,术中无血管、肠管、输尿管、膀胱及神经损伤,术后住院(4.47±1.02)d,无术后出血、血肿发生,留置导尿24~48 h,其中1例术后发生尿潴留,治疗后排尿通畅。随访6~24个月,未发现阴道网片暴露,无盆腔疼痛、新发压力性尿失禁及排便困难。术后1年复发1例。与术前相比,术后6个月Aa、Ba、C、D、Ap、Bp点的POP-Q值得到纠正,差异有统计学意义(P<0.01)。手术前后盆底障碍影响简易问卷7评分[(82.86±7.56)vs.(23.79±9.36)]、盆底功能障碍问卷表简表20评分[(65.41±11.10)vs.(23.47±10.57)]、盆腔器官脱垂/尿失禁性功能问卷表12评分[(32.70±10.30)vs.(15.70±7.48)]差异有统计学意义(P<0.01)。结论:腹腔镜髂耻韧带悬吊术易于分离及定位锚定点,手术并发症少,容易学习,尤其利于在基层医院推广应用,为治疗盆腔器官脱垂提供了新的术式选择。
文摘目的:对证据图研究进行文献计量学分析,以期了解目前证据图研究的现状并为学者开展相关研究提供借鉴和参考。方法:通过检索PubMed、EMBASE.com、Cochrane Library和 Web of Science获取证据图文献。利用VOSviewer 1.6.10软件对纳入研究的作者、国家、机构和关键词等信息进行提取,并对国家生成可视化合作网络图,对关键词进行聚类分析。采用CiteSpace V软件分析高产作者和高产机构之间的关系并生成可视化知识图谱。结果:共纳入证据图研究112篇,发文量最多的国家为美国(50篇),发文量最多的机构为塔夫茨大学(11篇),591位作者参与了证据图研究的发表,但发文量大于2篇的作者仅占4.74%。主要机构形成了3个研究团队,主要作者形成了6个合作团体,但各作者之间、机构之间合作较少。研究的主题主要有4个,主要的研究热点主题是肥胖、营养等人类的公共健康、婴幼儿的临床状况和青少年的心理健康。结论:高产的作者较少,参与研究的团体较多,各团体、机构之间的联系不够紧密,仍需进一步增强合作。证据图研究主题不多,仍需开拓新的研究主题。
