The dynamic response performance of a large,cylindrical,fluid-filled steel container under high-speed impact is evaluated through fluid-structure interaction analysis using arbitrary Lagrange-Eulerian(ALE)method.The A...The dynamic response performance of a large,cylindrical,fluid-filled steel container under high-speed impact is evaluated through fluid-structure interaction analysis using arbitrary Lagrange-Eulerian(ALE)method.The ALE method is adopted to accurately calculate the structural behavior induced by the internal liquid impact of the container.The stress and strain results obtained from the finite element analysis are in line with the experimental shell impact data.The influences of drop angle,drop height,and flow impact frequency are discussed.Calculation results indicate that the impact stress and damage of the container increase with drop height.However,the amplitude of the oscillation and the impact stress increase when the container and flow impact resonance occur at a certain drop height.The impact stress shows a nonlinear relationship with drop angle.展开更多
目的:探讨葛根素经p53/GLIPR1途径调节膀胱癌UMUC-3细胞增殖及侵袭的机制。方法:设膀胱癌UMUC-3细胞组、氟尿嘧啶组(80.0μg·ml^-1)、葛根素低、高剂量组(剂量分别为60.0μg·ml^-1、120.0μg·ml^-1),置于CO2培养箱(37℃...目的:探讨葛根素经p53/GLIPR1途径调节膀胱癌UMUC-3细胞增殖及侵袭的机制。方法:设膀胱癌UMUC-3细胞组、氟尿嘧啶组(80.0μg·ml^-1)、葛根素低、高剂量组(剂量分别为60.0μg·ml^-1、120.0μg·ml^-1),置于CO2培养箱(37℃、5%CO2、2%O2、93%N2)培养72 h。细胞培养结束后,测定各组UMUC-3细胞增殖水平、侵袭能力、凋亡率水平以及p53、GLIPR1基因蛋白水平。结果:氟尿嘧啶组、葛根素低、高剂量组吸光度(A)值、存活率水平、克隆形成数目、穿膜数、p53 m RNA、蛋白水平低于UMUC-3细胞组(P<0.05),凋亡率、GLIPR1 m RNA、蛋白水平高于UMUC-3细胞组(P<0.05);葛根素低剂量组A值、存活率水平、克隆形成数目、穿膜数、p53 m RNA、蛋白水平高于氟尿嘧啶组(P<0.05),凋亡率、GLIPR1 m RNA、蛋白水平低于氟尿嘧啶组(P<0.05);葛根素高剂量组A值、存活率水平、克隆形成数目、穿膜数、p53 m RNA、蛋白水平低于氟尿嘧啶组(P<0.05),凋亡率、GLIPR1 m RNA、蛋白水平高于氟尿嘧啶组(P<0.05)。结论:葛根素通过抑制p53 m RNA、蛋白的表达,促进GLIPR1 m RNA、蛋白的表达进而抑制膀胱癌细胞的增殖及侵袭,促进膀胱癌细胞的凋亡。展开更多
基金supported by the Prospective Joint Research Fund of the Jiangsu Provincial Department of Science and Technology Agency(No.BY2016073-11)
文摘The dynamic response performance of a large,cylindrical,fluid-filled steel container under high-speed impact is evaluated through fluid-structure interaction analysis using arbitrary Lagrange-Eulerian(ALE)method.The ALE method is adopted to accurately calculate the structural behavior induced by the internal liquid impact of the container.The stress and strain results obtained from the finite element analysis are in line with the experimental shell impact data.The influences of drop angle,drop height,and flow impact frequency are discussed.Calculation results indicate that the impact stress and damage of the container increase with drop height.However,the amplitude of the oscillation and the impact stress increase when the container and flow impact resonance occur at a certain drop height.The impact stress shows a nonlinear relationship with drop angle.
文摘目的:探讨葛根素经p53/GLIPR1途径调节膀胱癌UMUC-3细胞增殖及侵袭的机制。方法:设膀胱癌UMUC-3细胞组、氟尿嘧啶组(80.0μg·ml^-1)、葛根素低、高剂量组(剂量分别为60.0μg·ml^-1、120.0μg·ml^-1),置于CO2培养箱(37℃、5%CO2、2%O2、93%N2)培养72 h。细胞培养结束后,测定各组UMUC-3细胞增殖水平、侵袭能力、凋亡率水平以及p53、GLIPR1基因蛋白水平。结果:氟尿嘧啶组、葛根素低、高剂量组吸光度(A)值、存活率水平、克隆形成数目、穿膜数、p53 m RNA、蛋白水平低于UMUC-3细胞组(P<0.05),凋亡率、GLIPR1 m RNA、蛋白水平高于UMUC-3细胞组(P<0.05);葛根素低剂量组A值、存活率水平、克隆形成数目、穿膜数、p53 m RNA、蛋白水平高于氟尿嘧啶组(P<0.05),凋亡率、GLIPR1 m RNA、蛋白水平低于氟尿嘧啶组(P<0.05);葛根素高剂量组A值、存活率水平、克隆形成数目、穿膜数、p53 m RNA、蛋白水平低于氟尿嘧啶组(P<0.05),凋亡率、GLIPR1 m RNA、蛋白水平高于氟尿嘧啶组(P<0.05)。结论:葛根素通过抑制p53 m RNA、蛋白的表达,促进GLIPR1 m RNA、蛋白的表达进而抑制膀胱癌细胞的增殖及侵袭,促进膀胱癌细胞的凋亡。
