To investigate effects of nitric oxide on cellular radio-sensitivity, three human glioma cell lines, i.e. A172, A172 transfected green fluorescence protein (EGFP) gene (EA172) and A172 transfected inducible nitric oxi...To investigate effects of nitric oxide on cellular radio-sensitivity, three human glioma cell lines, i.e. A172, A172 transfected green fluorescence protein (EGFP) gene (EA172) and A172 transfected inducible nitric oxide synthesis (iNOS) gene (iA172), were irradiated by 12C6+ ions to 0, 1 or 2Gy. Productions of nitric oxide and glutathione (GSH) in A172, EA172 and iA172 were determined by chemical methods, cell cycle was analyzed by flow cytometry at the 24th hour after irradiation, and survival fraction of the cells was measured by colorimetric MTT assay at the 5th day after irradiation. The results showed that the concentrations of nitric oxide and GSH in iA172 were significantly higher than in A172 and EA172; the G2/M stage arrest induced by the 12C6+ ion irradiation was observed in A172 and EA172 but not in iA172 at the 24th hour after exposure; and the survival fraction of iA172 was higher than that of EA172 and iA172. Data suggest that the radio-sensitivity of the A172 was reduced after the iNOS gene transfection. The increase of GSH production and the change of cellular signals such as the cell cycle control induced by nitric oxide may be involved in this radio-resistance.展开更多
目的检索并总结肝移植患者术后益生菌强化的早期肠内营养使用的最佳证据,为肝移植术后早期肠内营养及益生菌个体化使用提供参考依据。方法根据PIPOST确定科学问题,检索British Medical Journal Best Practice、Cochrane Library、Jonna ...目的检索并总结肝移植患者术后益生菌强化的早期肠内营养使用的最佳证据,为肝移植术后早期肠内营养及益生菌个体化使用提供参考依据。方法根据PIPOST确定科学问题,检索British Medical Journal Best Practice、Cochrane Library、Jonna Briggs Institute、Guidelines International Network、National Guideline Clearinghouse等数据库,对文献证据评价及证据进行总结。结果本研究共纳入1篇证据总结、2篇系统评价及5篇随机对照研究,提取并总结出早期肠内营养时机、剂量、制剂类型、途径、益生菌剂量和种类6个维度共24条证据:术后24 h内开始早期肠内营养,使用要素膳,起始喂养速度20 mL/h,最快速度不超过125 mL/h,联合使用乳酸杆菌属20 mg、双歧杆菌属15 mg,3次/d,鼻饲尽早转换为经口进食,持续至少14 d。结论通过循证方法总结出肝移植患者术后益生菌强化的早期肠内营养使用的最佳证据,但我国关于肝移植术后早期肠内营养的研究较少,最佳证据的应用需要根据我国肝移植现状进行分析和实地策略构建。展开更多
Rhabdomyolysis is a syndrome which involves myoglobin and other intracellular proteins and electrolytes that occurs from breakdown of skeletal muscleand leak into the circulation. The clinical symptoms of rhabdomyolys...Rhabdomyolysis is a syndrome which involves myoglobin and other intracellular proteins and electrolytes that occurs from breakdown of skeletal muscleand leak into the circulation. The clinical symptoms of rhabdomyolysis include myalgia, tenderness on palpation, weakness, brown urine, fever, myoglobinuria, electrolyte imbalance, and acute renal failure. Rhabdomyolysis can be severe enough to be life threatening. The causes of rhabdomyolysis include alcohol, electrolyte imbalance （potassium, phosphorus, and magnesium depletion）, viral myositis, trauma, connective tissue diseases, drug reaction, toxication, exercise, metabolic diseases, seizures, and hereditary enzyme deficiencies. The exact mechanisms underlying rhabdomyolysis are not fully understood, but it is clear that muscle damage can be caused by direct muscle injury or metabolic inequalities between energy production and energy consumption.展开更多
基金grants from the National Natural Science Foundation of China (10675151)the Key Scientific Technology Research Projects of Gansu Prov-ince (2GS052-A43-00 8-02, 2GS063-A43-012)the Scientific Technology Research Project of Lanzhou-Chinese Academy of Sciences (06-2-58).
文摘To investigate effects of nitric oxide on cellular radio-sensitivity, three human glioma cell lines, i.e. A172, A172 transfected green fluorescence protein (EGFP) gene (EA172) and A172 transfected inducible nitric oxide synthesis (iNOS) gene (iA172), were irradiated by 12C6+ ions to 0, 1 or 2Gy. Productions of nitric oxide and glutathione (GSH) in A172, EA172 and iA172 were determined by chemical methods, cell cycle was analyzed by flow cytometry at the 24th hour after irradiation, and survival fraction of the cells was measured by colorimetric MTT assay at the 5th day after irradiation. The results showed that the concentrations of nitric oxide and GSH in iA172 were significantly higher than in A172 and EA172; the G2/M stage arrest induced by the 12C6+ ion irradiation was observed in A172 and EA172 but not in iA172 at the 24th hour after exposure; and the survival fraction of iA172 was higher than that of EA172 and iA172. Data suggest that the radio-sensitivity of the A172 was reduced after the iNOS gene transfection. The increase of GSH production and the change of cellular signals such as the cell cycle control induced by nitric oxide may be involved in this radio-resistance.
文摘Rhabdomyolysis is a syndrome which involves myoglobin and other intracellular proteins and electrolytes that occurs from breakdown of skeletal muscleand leak into the circulation. The clinical symptoms of rhabdomyolysis include myalgia, tenderness on palpation, weakness, brown urine, fever, myoglobinuria, electrolyte imbalance, and acute renal failure. Rhabdomyolysis can be severe enough to be life threatening. The causes of rhabdomyolysis include alcohol, electrolyte imbalance （potassium, phosphorus, and magnesium depletion）, viral myositis, trauma, connective tissue diseases, drug reaction, toxication, exercise, metabolic diseases, seizures, and hereditary enzyme deficiencies. The exact mechanisms underlying rhabdomyolysis are not fully understood, but it is clear that muscle damage can be caused by direct muscle injury or metabolic inequalities between energy production and energy consumption.
