Caspase-8 is an initiator of apoptotic signalling,and aberrations in procaspase-8 have been verified to be associated with malignant tumours.However,it remains to be confirmed whether chemotherapy resistance is relate...Caspase-8 is an initiator of apoptotic signalling,and aberrations in procaspase-8 have been verified to be associated with malignant tumours.However,it remains to be confirmed whether chemotherapy resistance is related to procaspase-8 aberrations in acute myeloid leukaemia(AML)patients.Procaspase-8 WT or mutant plasmids were constructed with molecular techniques and were transfected into HeLa cells with Lipofectamine.Apoptosis analysis was performed with annexin V-FITC in the cells overexpressing the WT or mutant procaspase-8 with or without TRAIL treatment.The cleavage of procaspase-8 or the expression of proteins downstream in the apoptotic cascade was detected by Western blotting.The dimerization of WT and mutant procaspase-8 proteins w as demonstrated with Co-IP.展开更多
In November 2016,Phumzile Mlambo-Ngcuka,Executive Director of UN Women,delivered the famous speech"Can the Sustainable Development Goals Deliver on their Promise of Gender Equality and the Empowerment of Women by...In November 2016,Phumzile Mlambo-Ngcuka,Executive Director of UN Women,delivered the famous speech"Can the Sustainable Development Goals Deliver on their Promise of Gender Equality and the Empowerment of Women by 2030?",which systematically expounded upon the relationship between gender equality and sustainable development in the arena of the UN.Ngcuka believes that all the 17 sustainable development goals mentioned in the report"Transforming our World by 2030-a New Agenda for Global Action"released by the United Nations in 2015 are of great significance for realizing gender equality.展开更多
文摘Caspase-8 is an initiator of apoptotic signalling,and aberrations in procaspase-8 have been verified to be associated with malignant tumours.However,it remains to be confirmed whether chemotherapy resistance is related to procaspase-8 aberrations in acute myeloid leukaemia(AML)patients.Procaspase-8 WT or mutant plasmids were constructed with molecular techniques and were transfected into HeLa cells with Lipofectamine.Apoptosis analysis was performed with annexin V-FITC in the cells overexpressing the WT or mutant procaspase-8 with or without TRAIL treatment.The cleavage of procaspase-8 or the expression of proteins downstream in the apoptotic cascade was detected by Western blotting.The dimerization of WT and mutant procaspase-8 proteins w as demonstrated with Co-IP.
文摘In November 2016,Phumzile Mlambo-Ngcuka,Executive Director of UN Women,delivered the famous speech"Can the Sustainable Development Goals Deliver on their Promise of Gender Equality and the Empowerment of Women by 2030?",which systematically expounded upon the relationship between gender equality and sustainable development in the arena of the UN.Ngcuka believes that all the 17 sustainable development goals mentioned in the report"Transforming our World by 2030-a New Agenda for Global Action"released by the United Nations in 2015 are of great significance for realizing gender equality.