The optimum conditions of supercritical fluid extraction(SFE) desorping the organic pollutants adsorbed and enriched by the adsorbent Porapak P and the effects of the conditions on the efficiencies ofSFE desorping o...The optimum conditions of supercritical fluid extraction(SFE) desorping the organic pollutants adsorbed and enriched by the adsorbent Porapak P and the effects of the conditions on the efficiencies ofSFE desorping organic pollutants are studied emphatically. Different kinds of organic adsorbents andmodifiers were tested. The recoveries of SFE are compared with Soxhlet extraction. The recoveries between93.1% and 109.3% are obtained for 25 kinds of compounds at 35 MPa, 100℃and 9.0ml SF-CO2, andtheir relative standard deviations (RSD) are in the range of 0.6-12.6 percent.展开更多
Overall wave loading and local hydrodynamic pressure distributions on a platform mat in regular waves for shallow waters are experimentally investigated in order to examine the severity of the nonlinear effects and sh...Overall wave loading and local hydrodynamic pressure distributions on a platform mat in regular waves for shallow waters are experimentally investigated in order to examine the severity of the nonlinear effects and shallow water effects. Wave focusing phenomenon is observed in the tests. The measured results may also provide a comparison basis for the theoretical development to consider the nonlinear interaction between waves and viscous flow by introducing viscosity into wave theories.展开更多
To explore correlation between the tk gene structure of pseudorabies virus (PRV) and its virulence, to study the effect of the gene mutation on PRV biological properties, and to investigate mechinism of reduced virule...To explore correlation between the tk gene structure of pseudorabies virus (PRV) and its virulence, to study the effect of the gene mutation on PRV biological properties, and to investigate mechinism of reduced virulence, thymidine kinase (TK)-deficient mutant of pseudorabies virus strain Hubei (PRV HB) was isolated by selection for resistance to 5-bromodeoxyuridine. The tk genes of PRV HB and its TK mutant were cloned and sequenced. 1587 base pairs of the tk gene and flanking regions of wild-type (wt) virus were sequenced, which included an open reading frame (ORF) of 1098 bp encoding a protein of 366 amino acids. The ORF contained two 137-bp repeated sequences, which were connected by an adenosine. 1458 bp of the tk and flanking regions of TK- mutant were sequenced. Analysis of the tk gene sequence of TK mutant indicated that one of 137 bp repeated sequence and the connecting adenosine in the tk gene of the wt virus was deleted and a repeated sequence of 8 nucleotides (GCGCGCC) was inserted. All展开更多
文摘The optimum conditions of supercritical fluid extraction(SFE) desorping the organic pollutants adsorbed and enriched by the adsorbent Porapak P and the effects of the conditions on the efficiencies ofSFE desorping organic pollutants are studied emphatically. Different kinds of organic adsorbents andmodifiers were tested. The recoveries of SFE are compared with Soxhlet extraction. The recoveries between93.1% and 109.3% are obtained for 25 kinds of compounds at 35 MPa, 100℃and 9.0ml SF-CO2, andtheir relative standard deviations (RSD) are in the range of 0.6-12.6 percent.
基金The project was financially supported by the Foundation of Doctorate Program of the State Education Commission of China
文摘Overall wave loading and local hydrodynamic pressure distributions on a platform mat in regular waves for shallow waters are experimentally investigated in order to examine the severity of the nonlinear effects and shallow water effects. Wave focusing phenomenon is observed in the tests. The measured results may also provide a comparison basis for the theoretical development to consider the nonlinear interaction between waves and viscous flow by introducing viscosity into wave theories.
文摘To explore correlation between the tk gene structure of pseudorabies virus (PRV) and its virulence, to study the effect of the gene mutation on PRV biological properties, and to investigate mechinism of reduced virulence, thymidine kinase (TK)-deficient mutant of pseudorabies virus strain Hubei (PRV HB) was isolated by selection for resistance to 5-bromodeoxyuridine. The tk genes of PRV HB and its TK mutant were cloned and sequenced. 1587 base pairs of the tk gene and flanking regions of wild-type (wt) virus were sequenced, which included an open reading frame (ORF) of 1098 bp encoding a protein of 366 amino acids. The ORF contained two 137-bp repeated sequences, which were connected by an adenosine. 1458 bp of the tk and flanking regions of TK- mutant were sequenced. Analysis of the tk gene sequence of TK mutant indicated that one of 137 bp repeated sequence and the connecting adenosine in the tk gene of the wt virus was deleted and a repeated sequence of 8 nucleotides (GCGCGCC) was inserted. All
