China is a country with high seismicity. It is very important for industry and structure to fortify against earthquakes. In this paper the outline of seismicity in China, the criteria for fortification against earthqu...China is a country with high seismicity. It is very important for industry and structure to fortify against earthquakes. In this paper the outline of seismicity in China, the criteria for fortification against earthquakes and the contents of seismic zonation map of China are described. The contents of seismic safety evaluation for major construction projects, such as large dams, large bridges, long distance pipe lines for transporting oil and natural gas, nuclear plants, petrochemical enterprises and so on, are presented. Some geological disasters caused by destructive earthquake, such as earthquake caused collapse and landslide, liquefaction of saturated soil and earthquake fault and so on, are also presented. Preventive countermeasures for these disasters are discussed.展开更多
Expression vector pBPC102, which carries winged bean lysine-rich protein (wblrp) gene and dihydropicolinate synthase (DHDPS) gene, was transferred into hexaploid winter wheat cv. Jinghua No.l, Jing411, You899 and Yang...Expression vector pBPC102, which carries winged bean lysine-rich protein (wblrp) gene and dihydropicolinate synthase (DHDPS) gene, was transferred into hexaploid winter wheat cv. Jinghua No.l, Jing411, You899 and Yangnongl5 explants of immature inflorescence and immature embryos by particle bombardment. More than 100 transgenic plants were obtained under the selection of s-(2-aminoethyl)-L-cysteine (AEC). Confirmed transgenic plants of To and TI generation by PCR and PCR-Southern blotting analyses showed successful integration of wblrp gene into wheat genome. Analysis of transgenic plant lines of T2 by Northern dot-blotting showed good expression of wblrp gene in offspring seed. The content of free lysine in leaves, contents of bound lysine and total proteins in seeds of T2 transgenie wheat lines were determined and analyzed. Among 34 tested transgenic lines, levels of free lysine content in leaves of 9 transgenic lines are 2~3times higher than un-trans-formed wild-type cultivars. Among 17 analyzed transgenic lines, bound lysine content of 4 transgenic lines is more than 10% higher than that of wild-type cultivars. Our research suggests that introducing wblrp gene into wheat is an effective way to improve its nutrition quality.展开更多
This paper characterizes all connected graphs with exactly two Laplacian eigenval-ues greater than two and all connected graphs with exactly one Laplacian eigenvalue greater than three.
文摘China is a country with high seismicity. It is very important for industry and structure to fortify against earthquakes. In this paper the outline of seismicity in China, the criteria for fortification against earthquakes and the contents of seismic zonation map of China are described. The contents of seismic safety evaluation for major construction projects, such as large dams, large bridges, long distance pipe lines for transporting oil and natural gas, nuclear plants, petrochemical enterprises and so on, are presented. Some geological disasters caused by destructive earthquake, such as earthquake caused collapse and landslide, liquefaction of saturated soil and earthquake fault and so on, are also presented. Preventive countermeasures for these disasters are discussed.
文摘Expression vector pBPC102, which carries winged bean lysine-rich protein (wblrp) gene and dihydropicolinate synthase (DHDPS) gene, was transferred into hexaploid winter wheat cv. Jinghua No.l, Jing411, You899 and Yangnongl5 explants of immature inflorescence and immature embryos by particle bombardment. More than 100 transgenic plants were obtained under the selection of s-(2-aminoethyl)-L-cysteine (AEC). Confirmed transgenic plants of To and TI generation by PCR and PCR-Southern blotting analyses showed successful integration of wblrp gene into wheat genome. Analysis of transgenic plant lines of T2 by Northern dot-blotting showed good expression of wblrp gene in offspring seed. The content of free lysine in leaves, contents of bound lysine and total proteins in seeds of T2 transgenie wheat lines were determined and analyzed. Among 34 tested transgenic lines, levels of free lysine content in leaves of 9 transgenic lines are 2~3times higher than un-trans-formed wild-type cultivars. Among 17 analyzed transgenic lines, bound lysine content of 4 transgenic lines is more than 10% higher than that of wild-type cultivars. Our research suggests that introducing wblrp gene into wheat is an effective way to improve its nutrition quality.
基金the National Natural Science Foundation of Chinathe Scientific ResearchFoundation for the Returned Overseas Chinese Scholars the Ministry of Education of China.
文摘This paper characterizes all connected graphs with exactly two Laplacian eigenval-ues greater than two and all connected graphs with exactly one Laplacian eigenvalue greater than three.
