The management of oral and maxillofacial tissue defects caused by tumors,trauma,and congenital or acquired deformities has been a major challenge for surgeons over the last few decades.Autologous tissue transplantatio...The management of oral and maxillofacial tissue defects caused by tumors,trauma,and congenital or acquired deformities has been a major challenge for surgeons over the last few decades.Autologous tissue transplantation,the gold standard of tissue reconstruction,is a valid method for repairing the oral and maxillofacial functions and aesthetics.However,several limitations hinder its clinical applications including complications of donor sites,limited tissue volume,and uncertain long-term outcomes.Adipose-derived mesenchymal stem cells(ADMSCs)widely exist in adipose tissue and can be easily obtained through liposuction.Like the bone marrow-derived mesenchymal stem cells(BMSCs),ADMSCs also have the multi-pluripotent potencies to differentiate into osteoblasts,chondrocytes,neurons,and myocytes.Therefore,the multilineage capacity of ADMSCs makes them valuable for cell-based medical therapies.In recent years,researchers have developed many candidates of ADMSCs-based biomaterial scaffolds to cater for the needs of oral and maxillofacial tissue engineering due to their superior performance.This review presents the advances and applications of ADMSCs-based biomaterial scaffolds,and explores their tissue engineering prospects in oral and maxillofacial reconstructions.展开更多
Nowadays, there are still many challenges to skin regeneration. As a new type of skin substitute, hydrogel has emerging gradually with its excellent properties. However, it is still a challenge to combine with biologi...Nowadays, there are still many challenges to skin regeneration. As a new type of skin substitute, hydrogel has emerging gradually with its excellent properties. However, it is still a challenge to combine with biological active agents to facilitate skin regeneration. Under the circumstance, we synthesized argininebased poly(ester amide)(Arg-PEA) and hyaluronic acid(HA-MA), and combined them into new hybrid hydrogels via photo-crosslinking. We found that the internal structure and physicochemical properties of hybrid hydrogels were greatly improved with the increase of content of Arg-PEA. Therefore, we designed hybrid hydrogels with 5 wt% and 10 wt% of Arg-PEA content, respectively. Besides, we selected the corresponding anti-inflammatory(CRP, TNF-α) indicators to detect the anti-inflammatory properties of the hybrid hydrogels at the protein level, and the corresponding antioxidant indicators(SOD, GSH/GSSG, MDA)were selected to investigate the antioxidant properties of hybrid hydrogels at the cellular level in vitro.In addition, we also selected relevant genes to test the effect of hybrid hydrogels on fibrosis and vascularization in the process of skin wound healing in vitro and verified them in vivo with a mouse dorsum wound model. The results confirmed that Arg-PEA/HA-MA(AH) hybrid hydrogel was a prospective scaffold material for skin regeneration.展开更多
文摘The management of oral and maxillofacial tissue defects caused by tumors,trauma,and congenital or acquired deformities has been a major challenge for surgeons over the last few decades.Autologous tissue transplantation,the gold standard of tissue reconstruction,is a valid method for repairing the oral and maxillofacial functions and aesthetics.However,several limitations hinder its clinical applications including complications of donor sites,limited tissue volume,and uncertain long-term outcomes.Adipose-derived mesenchymal stem cells(ADMSCs)widely exist in adipose tissue and can be easily obtained through liposuction.Like the bone marrow-derived mesenchymal stem cells(BMSCs),ADMSCs also have the multi-pluripotent potencies to differentiate into osteoblasts,chondrocytes,neurons,and myocytes.Therefore,the multilineage capacity of ADMSCs makes them valuable for cell-based medical therapies.In recent years,researchers have developed many candidates of ADMSCs-based biomaterial scaffolds to cater for the needs of oral and maxillofacial tissue engineering due to their superior performance.This review presents the advances and applications of ADMSCs-based biomaterial scaffolds,and explores their tissue engineering prospects in oral and maxillofacial reconstructions.
基金supported by the National Natural Science Foundation of China (No. 52103039)Sichuan University postdoctoral interdisciplinary Innovation Fund。
文摘Nowadays, there are still many challenges to skin regeneration. As a new type of skin substitute, hydrogel has emerging gradually with its excellent properties. However, it is still a challenge to combine with biological active agents to facilitate skin regeneration. Under the circumstance, we synthesized argininebased poly(ester amide)(Arg-PEA) and hyaluronic acid(HA-MA), and combined them into new hybrid hydrogels via photo-crosslinking. We found that the internal structure and physicochemical properties of hybrid hydrogels were greatly improved with the increase of content of Arg-PEA. Therefore, we designed hybrid hydrogels with 5 wt% and 10 wt% of Arg-PEA content, respectively. Besides, we selected the corresponding anti-inflammatory(CRP, TNF-α) indicators to detect the anti-inflammatory properties of the hybrid hydrogels at the protein level, and the corresponding antioxidant indicators(SOD, GSH/GSSG, MDA)were selected to investigate the antioxidant properties of hybrid hydrogels at the cellular level in vitro.In addition, we also selected relevant genes to test the effect of hybrid hydrogels on fibrosis and vascularization in the process of skin wound healing in vitro and verified them in vivo with a mouse dorsum wound model. The results confirmed that Arg-PEA/HA-MA(AH) hybrid hydrogel was a prospective scaffold material for skin regeneration.
