Clostridium peringens, also called clostridium westergren, mainly causes diseases in ruminants, such as gas gangrene, enterotoxaemia, hemorrhagic enteritis of cattle and sheep. The bacteria are divided into five subty...Clostridium peringens, also called clostridium westergren, mainly causes diseases in ruminants, such as gas gangrene, enterotoxaemia, hemorrhagic enteritis of cattle and sheep. The bacteria are divided into five subtypes including A, B, C, D and E subtype, which can secrete more than 10 kinds of exotoxin,In these toxins, a, β, ε and ζ play a key pathogenic role. At present, the disease is prevented mainly by univalent vaccine, combined vaccine and muhivalent inactivated vaccine. And the genetic engineering subunit vaccine and nucleic acid vaccine has wide develop- ment prospects. In order to provide some reference for comprehensive prevention and deep research of C. pe^ringens, the etiological characteristics of C. perfrin- germ, pathogenic mechanism of exotoxin and research progress of related vaccines were reviewed, and the genetic engineering subunit vaccine and nucleic acid vac- cine development prospects were also discussed in this paper.展开更多
[Objective] The paper was to develop genetic engineering vaccine that can express α exotoxin antigen protein efficiently without destroying its immunogenicity for preventing and controlling the diseases caused by Clo...[Objective] The paper was to develop genetic engineering vaccine that can express α exotoxin antigen protein efficiently without destroying its immunogenicity for preventing and controlling the diseases caused by Clostridium perfringens. [Method] Efficiently expressed soluble recombinant α protein was obtained from Escherichia coli expression system by optimizing codon,removing signal peptide,selecting sequences with better hydrophilicity and antigenicity,and optimizing expression conditions. [Result] Mice obtained higher serum antibody level when immunized by α protein,and the immune protection rates against type A,type B,type C and type D C. perfringens were 100%,90%,85% and 90%,respectively. The antibody titer of mice within 7-14 d after the third immunization reached the peak. [Conclusion]The α protein has good immunogenicity,and can be further used to develop genetic engineering subunit vaccines for preventing C. perfringens.展开更多
[ Objective] The paper was to get effective soluble N protein to establish indirect ELISA method for Peste des petits ruminants （PPR）. [ Method] Soluble N protein with high expression was obtained from Escherichia c...[ Objective] The paper was to get effective soluble N protein to establish indirect ELISA method for Peste des petits ruminants （PPR）. [ Method] Soluble N protein with high expression was obtained from Escherichia coli expression system through codon optimization and optimization of expression conditions, and indirect ELISA detection method based on N protein was further established. [ Result] The assay had no cross reaction with other sheep pathogens. The intra- and inter-batch variation coefficients were less than 9%, indicating the method had good repeatability. Furthermore, totally 480 clinical serum samples were detec- ted by the assay, and the agreement rate with commercial ELISA kit （IDVET） was 98.33%. [ Conclusion] The study laid a foundation for further development of mature PPRV antibody detection kits.展开更多
The photoelectrochemical properties of semiconductors mainly depend on the size and shape of the corresponding nanoparticles.Herein,3D WO3 nanosheets were controllably synthesized with the aid of polyethyleneimine,whi...The photoelectrochemical properties of semiconductors mainly depend on the size and shape of the corresponding nanoparticles.Herein,3D WO3 nanosheets were controllably synthesized with the aid of polyethyleneimine,which presents enhanced photocurrent responses.Based on this excellent photoelectrochemical property,a photoelectrochemical chip was prepared by lithography technology for the smart monitoring of the antioxidant capacity(AC)in red wine and exhibits a series of advantages including rapid response time,high sensitivity,and long-lasting stability.The mechanism of the present photoelectrochemical sensing was explored and shows a single electron transfer reaction.Furthermore,only 200µL of samples are required for one testing,which demonstrates that the present photoelectrochemical chip can be potentially integrated with a portable commercial device(such as a mobile phone)for further research and development of food and drug supervision.展开更多
基金Supported by Monitoring and Control Program of Animal Diseases of the Ministry of Agriculture
文摘Clostridium peringens, also called clostridium westergren, mainly causes diseases in ruminants, such as gas gangrene, enterotoxaemia, hemorrhagic enteritis of cattle and sheep. The bacteria are divided into five subtypes including A, B, C, D and E subtype, which can secrete more than 10 kinds of exotoxin,In these toxins, a, β, ε and ζ play a key pathogenic role. At present, the disease is prevented mainly by univalent vaccine, combined vaccine and muhivalent inactivated vaccine. And the genetic engineering subunit vaccine and nucleic acid vaccine has wide develop- ment prospects. In order to provide some reference for comprehensive prevention and deep research of C. pe^ringens, the etiological characteristics of C. perfrin- germ, pathogenic mechanism of exotoxin and research progress of related vaccines were reviewed, and the genetic engineering subunit vaccine and nucleic acid vac- cine development prospects were also discussed in this paper.
基金Supported by the 13th Five-Year National Key Research and Development Program(2016YFD0500901)
文摘[Objective] The paper was to develop genetic engineering vaccine that can express α exotoxin antigen protein efficiently without destroying its immunogenicity for preventing and controlling the diseases caused by Clostridium perfringens. [Method] Efficiently expressed soluble recombinant α protein was obtained from Escherichia coli expression system by optimizing codon,removing signal peptide,selecting sequences with better hydrophilicity and antigenicity,and optimizing expression conditions. [Result] Mice obtained higher serum antibody level when immunized by α protein,and the immune protection rates against type A,type B,type C and type D C. perfringens were 100%,90%,85% and 90%,respectively. The antibody titer of mice within 7-14 d after the third immunization reached the peak. [Conclusion]The α protein has good immunogenicity,and can be further used to develop genetic engineering subunit vaccines for preventing C. perfringens.
基金Supported by New Diagnosis and Detection Technology Research for Major Animal Diseases in Cattle and Sheep(No.2016YFD0500901)
文摘[ Objective] The paper was to get effective soluble N protein to establish indirect ELISA method for Peste des petits ruminants （PPR）. [ Method] Soluble N protein with high expression was obtained from Escherichia coli expression system through codon optimization and optimization of expression conditions, and indirect ELISA detection method based on N protein was further established. [ Result] The assay had no cross reaction with other sheep pathogens. The intra- and inter-batch variation coefficients were less than 9%, indicating the method had good repeatability. Furthermore, totally 480 clinical serum samples were detec- ted by the assay, and the agreement rate with commercial ELISA kit （IDVET） was 98.33%. [ Conclusion] The study laid a foundation for further development of mature PPRV antibody detection kits.
基金Research&Development Projects in Key Areas of Guangdong Province,China(2019B010933001)National Natural Science Foundation of China(21222402)+2 种基金National Basic Research Program of China(973 Program,2017YFE0197900)Innovation Training Program for College Students of Guangzhou University(XJ202011078036)Guangzhou Science and Technology Plan Project(202102010390)。
基金This work was supported by the National Natural Science Foundation of China(Nos.21527806,21627809,21727815)the Project of the Department of Science and Techniques of Guangdong Province,China(No.2019B010933001)the Project of the Department of Science and Techniques of Jilin Province,China(Nos.20170442014SC,20170203004SF,20170101183JC,20180201021GX).
文摘The photoelectrochemical properties of semiconductors mainly depend on the size and shape of the corresponding nanoparticles.Herein,3D WO3 nanosheets were controllably synthesized with the aid of polyethyleneimine,which presents enhanced photocurrent responses.Based on this excellent photoelectrochemical property,a photoelectrochemical chip was prepared by lithography technology for the smart monitoring of the antioxidant capacity(AC)in red wine and exhibits a series of advantages including rapid response time,high sensitivity,and long-lasting stability.The mechanism of the present photoelectrochemical sensing was explored and shows a single electron transfer reaction.Furthermore,only 200µL of samples are required for one testing,which demonstrates that the present photoelectrochemical chip can be potentially integrated with a portable commercial device(such as a mobile phone)for further research and development of food and drug supervision.
