Objective:To investigate the mechanism of Fuyang Jiebiao granule(FYJBKL)in the treatment of viral pneumonia.Methods:Firstly,a network model was constructed using network pharmacology to study the target expression sit...Objective:To investigate the mechanism of Fuyang Jiebiao granule(FYJBKL)in the treatment of viral pneumonia.Methods:Firstly,a network model was constructed using network pharmacology to study the target expression sites of FYJBKL viral pneumonia,so as to determine the main targets and important signal transduction pathways for the treatment of viral pneumonia.Secondly,the main components of the drug and the main target are docked.Then,the fever,sweating and inflammation rat models were established to explore the antipyretic,sweating and anti-inflammatory mechanisms of FYJBKL.Finally,the contents of IL-17,IL-1β,TNF-αand IL-6 in blood samples of rats were analyzed by ELISA method,and the morphological changes of lung tissue were observed by HE staining.Results:Quercetin,luteolin,kaempferol,etc.,and the main mechanism targets are IL-17,IL-1β,TNF-α,IL-6 and so on.Thirty signal pathways were identified by KEGG enrichment analysis,including interleukin-17 signaling pathway(IL-17 signaling pathway),human cytomegalovirus infection pathway(human cytomegalovirus infection),Kaposi's sarcoma associated herpesvirus infection pathway(Kaposi's sarcoma-as-sociated herpesvirus infection)and so on.After the study of molecular docking,we found that the contact efficiency between active substances and possible key targets is good.The high and middle concentration groups of FYJBKL significantly decreased the expression of IL-17,IL-1β,TNF-αand IL-6 in the blood of rats with inflammation(P<0.05).FYJBKL significantly reduced the foot swelling induced by egg white and inhibited the increase of body temperature induced by yeast in rats(P<0.05).HE staining showed that FYJBKL improved pulmonary fibrosis and inflammatory exudation to varying degrees.Conclusion:The effects of FuyangJiebiao granules on the related signal pathways of anti-virus,anti-immune and anti-inflammation as well as biological and cellular processes may be caused by the binding of quercetin,luteolin,kaempferol and other active ingredients to their shared targets.Fuyang Jiebiao granules can improve the related symptoms caused by viral pneumonia,and its mechanism may be related to the activities of TNF,IL-17,IL-6 and other related channels,which are multiple targets of inflammation regulation.展开更多
目的:探讨重组人粒细胞集落刺激因子(G-CSF)联合左氧氟沙星或仅用G-CSF支持疗法在预防自体造血干细胞移植感染中的作用,分析移植患者住院时间、住院费用及移植后生存情况。方法:回顾性分析2012年1月至2022年7月于本院就诊的接受自体造...目的:探讨重组人粒细胞集落刺激因子(G-CSF)联合左氧氟沙星或仅用G-CSF支持疗法在预防自体造血干细胞移植感染中的作用,分析移植患者住院时间、住院费用及移植后生存情况。方法:回顾性分析2012年1月至2022年7月于本院就诊的接受自体造血干细胞移植的恶性血液病患者,观察自体造血干细胞移植期间左氧氟沙星+G-CSF预防感染组和G-CSF支持组中性粒细胞缺乏伴发热、细菌感染发生率及静脉抗菌药物使用情况,并比较两组住院时间、住院期间总费用及移植90天后存活情况。结果:102例患者纳入研究,其中多发性骨髓瘤54例,急性白血病36例,淋巴瘤7例,骨髓增生异常综合征3例,轻链型淀粉样变性1例,POEMS综合征1例。47例接受G-CSF+左氧氟沙星预防感染,55例接受G-CSF支持治疗。左氧氟沙星+G-CSF组中40例(85.11%)发生中性粒细胞缺乏伴发热,13例(27.66%)明确为细菌感染。G-CSF组中44例(80.00%)发生中性粒细胞缺乏伴发热,16例(29.09%)细菌感染。两组中性粒细胞缺乏伴发热和细菌感染发生率均无统计学差异(χ^(2)=0.46,P=0.50;χ^(2)=0.03,P=0.87)。左氧氟沙星+G-CSF组静脉抗菌药物使用率为85.11%(40/47),与G-CSF组的85.45%(47/55)比较无统计学差异(χ^(2)=0.04,P=0.84)。左氧氟沙星+G-CSF组与G-CSF组左氧氟沙星耐药菌的检出率分别为8.57%(3/35)和21.43%(6/28),无统计学差异(χ^(2)=0.65,P>0.05)。左氧氟沙星+G-CSF与G-CSF组患者住院中位时间为25 d vs 22 d,住院中位费用为78216.24元vs 80724.38元,差异均无统计学意义(t=3.00,P=0.09;t=0.94,P=0.09)。移植后90天内,左氧氟沙星+G-CSF组有2例(4.26%)死亡,G-CSF组有1例(1.82%)死亡,两组比较无统计学差异(χ^(2)=0.53,P=0.47)。结论:较G-CSF支持相比,患者在自体造血干细胞移植期间应用G-CSF+左氧氟沙星预防感染无明显获益。展开更多
为明确关键栽培措施对菜籽油品质的影响,本研究以“湘杂油518”和“大地199”为材料,分别在武汉和兰州进行播期、密度及氮肥处理单因素试验,籽粒成熟后收获冷榨,测定菜籽油色泽、过氧化值、极性总酚及甾醇含量等关键品质指标,综合评价...为明确关键栽培措施对菜籽油品质的影响,本研究以“湘杂油518”和“大地199”为材料,分别在武汉和兰州进行播期、密度及氮肥处理单因素试验,籽粒成熟后收获冷榨,测定菜籽油色泽、过氧化值、极性总酚及甾醇含量等关键品质指标,综合评价各处理下菜籽油品质。结果表明:本试验条件下,播期、密度、氮肥均显著影响菜籽油关键品质指标,但各指标在两品种、两试验点间的变化规律不尽相同。两试点、两品种均在早播条件下菜籽油的油酸含量较高,且均随播期推迟,色泽加深,叶绿素含量升高,综合品质下降。两试点的湘杂油518品种在D2密度(4.5×10^(5)hm^(–2))下的菜籽油的酸价较低,极性总酚、总植物甾醇和总生育酚含量较高,综合品质较好。两试点的大地199品种则随密度增加菜籽油叶绿素含量增加,色泽加深,亚油酸含量降低,综合品质下降。两试点、两品种均在低氮下(120 kg hm^(–2))有较高的籽粒含油量和出油效率,且均随施氮量的增加菜籽油色泽加深,综合品质下降。主成分分析表明,播期对菜籽油综合品质的影响大于种植密度和施氮量处理,酸价、叶绿素、极性总酚、抗氧化能力和总植物甾醇是影响菜籽油的关键品质指标;相关性分析表明,籽粒含油量与出油效率显著正相关,叶绿素、类胡萝卜素与菜籽油色泽均呈显著正相关,极性总酚与抗氧化性显著正相关。综上所述,生产中适期早播、减少氮肥用量、降低种植密度,可提高压榨菜籽油品质。本试验结果可为高品质菜籽油优质原料的生产提供技术支撑。展开更多
基金Emergency Research Project for Novel Coronavirus(2019-nCoV)Prevention and Control in Shanxi Province(No.202003D31012/GZ)Jingfang Fuyang Key Laboratory of Shanxi Province(No.202104010910011)Shanxi Provincial Health Commission Key Laboratory Construction Project。
文摘Objective:To investigate the mechanism of Fuyang Jiebiao granule(FYJBKL)in the treatment of viral pneumonia.Methods:Firstly,a network model was constructed using network pharmacology to study the target expression sites of FYJBKL viral pneumonia,so as to determine the main targets and important signal transduction pathways for the treatment of viral pneumonia.Secondly,the main components of the drug and the main target are docked.Then,the fever,sweating and inflammation rat models were established to explore the antipyretic,sweating and anti-inflammatory mechanisms of FYJBKL.Finally,the contents of IL-17,IL-1β,TNF-αand IL-6 in blood samples of rats were analyzed by ELISA method,and the morphological changes of lung tissue were observed by HE staining.Results:Quercetin,luteolin,kaempferol,etc.,and the main mechanism targets are IL-17,IL-1β,TNF-α,IL-6 and so on.Thirty signal pathways were identified by KEGG enrichment analysis,including interleukin-17 signaling pathway(IL-17 signaling pathway),human cytomegalovirus infection pathway(human cytomegalovirus infection),Kaposi's sarcoma associated herpesvirus infection pathway(Kaposi's sarcoma-as-sociated herpesvirus infection)and so on.After the study of molecular docking,we found that the contact efficiency between active substances and possible key targets is good.The high and middle concentration groups of FYJBKL significantly decreased the expression of IL-17,IL-1β,TNF-αand IL-6 in the blood of rats with inflammation(P<0.05).FYJBKL significantly reduced the foot swelling induced by egg white and inhibited the increase of body temperature induced by yeast in rats(P<0.05).HE staining showed that FYJBKL improved pulmonary fibrosis and inflammatory exudation to varying degrees.Conclusion:The effects of FuyangJiebiao granules on the related signal pathways of anti-virus,anti-immune and anti-inflammation as well as biological and cellular processes may be caused by the binding of quercetin,luteolin,kaempferol and other active ingredients to their shared targets.Fuyang Jiebiao granules can improve the related symptoms caused by viral pneumonia,and its mechanism may be related to the activities of TNF,IL-17,IL-6 and other related channels,which are multiple targets of inflammation regulation.
文摘目的:探讨重组人粒细胞集落刺激因子(G-CSF)联合左氧氟沙星或仅用G-CSF支持疗法在预防自体造血干细胞移植感染中的作用,分析移植患者住院时间、住院费用及移植后生存情况。方法:回顾性分析2012年1月至2022年7月于本院就诊的接受自体造血干细胞移植的恶性血液病患者,观察自体造血干细胞移植期间左氧氟沙星+G-CSF预防感染组和G-CSF支持组中性粒细胞缺乏伴发热、细菌感染发生率及静脉抗菌药物使用情况,并比较两组住院时间、住院期间总费用及移植90天后存活情况。结果:102例患者纳入研究,其中多发性骨髓瘤54例,急性白血病36例,淋巴瘤7例,骨髓增生异常综合征3例,轻链型淀粉样变性1例,POEMS综合征1例。47例接受G-CSF+左氧氟沙星预防感染,55例接受G-CSF支持治疗。左氧氟沙星+G-CSF组中40例(85.11%)发生中性粒细胞缺乏伴发热,13例(27.66%)明确为细菌感染。G-CSF组中44例(80.00%)发生中性粒细胞缺乏伴发热,16例(29.09%)细菌感染。两组中性粒细胞缺乏伴发热和细菌感染发生率均无统计学差异(χ^(2)=0.46,P=0.50;χ^(2)=0.03,P=0.87)。左氧氟沙星+G-CSF组静脉抗菌药物使用率为85.11%(40/47),与G-CSF组的85.45%(47/55)比较无统计学差异(χ^(2)=0.04,P=0.84)。左氧氟沙星+G-CSF组与G-CSF组左氧氟沙星耐药菌的检出率分别为8.57%(3/35)和21.43%(6/28),无统计学差异(χ^(2)=0.65,P>0.05)。左氧氟沙星+G-CSF与G-CSF组患者住院中位时间为25 d vs 22 d,住院中位费用为78216.24元vs 80724.38元,差异均无统计学意义(t=3.00,P=0.09;t=0.94,P=0.09)。移植后90天内,左氧氟沙星+G-CSF组有2例(4.26%)死亡,G-CSF组有1例(1.82%)死亡,两组比较无统计学差异(χ^(2)=0.53,P=0.47)。结论:较G-CSF支持相比,患者在自体造血干细胞移植期间应用G-CSF+左氧氟沙星预防感染无明显获益。
文摘为明确关键栽培措施对菜籽油品质的影响,本研究以“湘杂油518”和“大地199”为材料,分别在武汉和兰州进行播期、密度及氮肥处理单因素试验,籽粒成熟后收获冷榨,测定菜籽油色泽、过氧化值、极性总酚及甾醇含量等关键品质指标,综合评价各处理下菜籽油品质。结果表明:本试验条件下,播期、密度、氮肥均显著影响菜籽油关键品质指标,但各指标在两品种、两试验点间的变化规律不尽相同。两试点、两品种均在早播条件下菜籽油的油酸含量较高,且均随播期推迟,色泽加深,叶绿素含量升高,综合品质下降。两试点的湘杂油518品种在D2密度(4.5×10^(5)hm^(–2))下的菜籽油的酸价较低,极性总酚、总植物甾醇和总生育酚含量较高,综合品质较好。两试点的大地199品种则随密度增加菜籽油叶绿素含量增加,色泽加深,亚油酸含量降低,综合品质下降。两试点、两品种均在低氮下(120 kg hm^(–2))有较高的籽粒含油量和出油效率,且均随施氮量的增加菜籽油色泽加深,综合品质下降。主成分分析表明,播期对菜籽油综合品质的影响大于种植密度和施氮量处理,酸价、叶绿素、极性总酚、抗氧化能力和总植物甾醇是影响菜籽油的关键品质指标;相关性分析表明,籽粒含油量与出油效率显著正相关,叶绿素、类胡萝卜素与菜籽油色泽均呈显著正相关,极性总酚与抗氧化性显著正相关。综上所述,生产中适期早播、减少氮肥用量、降低种植密度,可提高压榨菜籽油品质。本试验结果可为高品质菜籽油优质原料的生产提供技术支撑。
