The aim of this study was to detect evidence for signatures of recent selection in the Jinhua pig genome.These results can be useful to better understand the regions under selection in Jinhua pigs and might shed some ...The aim of this study was to detect evidence for signatures of recent selection in the Jinhua pig genome.These results can be useful to better understand the regions under selection in Jinhua pigs and might shed some lights on groups of genes that control production traits.In the present study,we performed extended haplotype homozygosity(EHH)tests to identify significant core regions in 202 Jinhua pigs.A total of 26161 core regions spanning 636.42 Mb were identified,which occupied approximately 28%of the genome across all autosomes,and 1158 significant(P<0.01)core haplotypes were selected.Genes in these regions were related to several economically important traits,including meat quality,reproduction,immune responses and exterior traits.A panel of genes including ssc-mir-365-2,KDM8,RABEP2,GSG1L,RHEB,RPH3AL and a signal pathway of PI3K-Akt were detected with the most extreme P-values.The findings in our study could draw a comparatively genome-wide map of selection signature in the pig genome,and also help to detect functional candidate genes under positive selection for further genetic and breeding research in Jinhua and other pigs.展开更多
The present study was designed to isolate and characterize a purified extract from Fusarium solani FG319,termed MFS(Metabolite of Fusarium solani FG319) that showed anti-atherosclerosis activity by inhibiting 3-hydrox...The present study was designed to isolate and characterize a purified extract from Fusarium solani FG319,termed MFS(Metabolite of Fusarium solani FG319) that showed anti-atherosclerosis activity by inhibiting 3-hydroxy-3-methylglutaryl coenzyme A(HMG-Co A) reductase.Response surface methodology(RSM) was employed to achieve an improved yield from the fermentation medium.The inhibiting effect of the isolate,MFS,on HMG-Co A reductase was greater than that of the positive control,lovastatin.The average recovery of MFS and the relative standard deviation(RSD) ranged between 99.75% to 101.18%,and 0.31% to 0.74%,respectively.The RSDs intra-and inter-assay of the three samples ranged from 0.288% to 2.438%,and from 0.934% to 2.383%,respectively.From the RSM,the concentration of inducer,cultivation time,and culture temperatures had significant effects on the MFS production,with the effect of inducer concentration being more pronounced that other factors.In conclusion,the optimal conditions for the MFS production were achieved using RSM and that MFS could be explored as an anti-atherosclerosis agent based on its ability to inhibit HMG-Co A reductase.展开更多
基金supported by the Key Technology R&D Program of China(2016C02054-2)the National Natural Science Foundation of China(31872976)。
文摘The aim of this study was to detect evidence for signatures of recent selection in the Jinhua pig genome.These results can be useful to better understand the regions under selection in Jinhua pigs and might shed some lights on groups of genes that control production traits.In the present study,we performed extended haplotype homozygosity(EHH)tests to identify significant core regions in 202 Jinhua pigs.A total of 26161 core regions spanning 636.42 Mb were identified,which occupied approximately 28%of the genome across all autosomes,and 1158 significant(P<0.01)core haplotypes were selected.Genes in these regions were related to several economically important traits,including meat quality,reproduction,immune responses and exterior traits.A panel of genes including ssc-mir-365-2,KDM8,RABEP2,GSG1L,RHEB,RPH3AL and a signal pathway of PI3K-Akt were detected with the most extreme P-values.The findings in our study could draw a comparatively genome-wide map of selection signature in the pig genome,and also help to detect functional candidate genes under positive selection for further genetic and breeding research in Jinhua and other pigs.
基金supported by National High Technology Research and Development Program of China(No.2011AA09070109)Marine Pharmaceutical-food Science Interdisciplinary Project of Shanghai Ocean University
文摘The present study was designed to isolate and characterize a purified extract from Fusarium solani FG319,termed MFS(Metabolite of Fusarium solani FG319) that showed anti-atherosclerosis activity by inhibiting 3-hydroxy-3-methylglutaryl coenzyme A(HMG-Co A) reductase.Response surface methodology(RSM) was employed to achieve an improved yield from the fermentation medium.The inhibiting effect of the isolate,MFS,on HMG-Co A reductase was greater than that of the positive control,lovastatin.The average recovery of MFS and the relative standard deviation(RSD) ranged between 99.75% to 101.18%,and 0.31% to 0.74%,respectively.The RSDs intra-and inter-assay of the three samples ranged from 0.288% to 2.438%,and from 0.934% to 2.383%,respectively.From the RSM,the concentration of inducer,cultivation time,and culture temperatures had significant effects on the MFS production,with the effect of inducer concentration being more pronounced that other factors.In conclusion,the optimal conditions for the MFS production were achieved using RSM and that MFS could be explored as an anti-atherosclerosis agent based on its ability to inhibit HMG-Co A reductase.
