Suzhong swine is a hybrid breed derived from Taihu sows and Landraee boars. To identify Suzhong swine individuals and trace the source of pork products, single nucleotide polymorphisms （SNPs） identification of Suzho...Suzhong swine is a hybrid breed derived from Taihu sows and Landraee boars. To identify Suzhong swine individuals and trace the source of pork products, single nucleotide polymorphisms （SNPs） identification of Suzhong swine individuals was studied. A total of 29 pairs of primers were designed and sev- en pairs of primers were used for identification of Suzhong swine individuals. The products amplified by seven pairs of primers could be directly sequenced, with clean sequencing map background and no ambiguity in sequence read. Totally 52 SNPs loci were amplified by seven pairs of primers, and 41 SNPs loci were reserved for identification of Suzhong swine individuals through correlation analysis and heterezygosity filtration （ H ≥0.1 ）. Meantime, the digital barcodes for SNP identification of 96 individuals of Suzhong swine derived from seven boars and 12 sows were developed, which well distinguished 96 individuals of Suzhong swine. Theoretically, 41SNPs amplified by seven pairs of primers could be used for identification of 5.0 × 10^6 pig individuals. Therefore, digital barcode devel- opment method for SNP identification of Suzbong swine individuals can be used for individual identification of Suzhong swine in scale pig farm and meat product traceability.展开更多
Myeloid differentiation protein-88(MyD88) is a crucial adaptor protein in the innate immune response.A protective role for MyD88 in normal cardiac function has been proposed in a surgical hypertrophic model.To assess ...Myeloid differentiation protein-88(MyD88) is a crucial adaptor protein in the innate immune response.A protective role for MyD88 in normal cardiac function has been proposed in a surgical hypertrophic model.To assess the in vivo role of MyD88 in cardiac remodeling,we generated transgenic mice with cardiac-restricted expression of a dominant negative mutant of MyD88(dnMyD88).Surprisingly,dnMyD88 transgenic mice displayed characteristic features of heart failure;including heart weight increase,cardiomyocytes enlargement,interstitial fibrosis,and re-expression of "fetal" genes.Echocardiographic examination of dnMyD88 hearts revealed dilated chamber volume and reduced cardiac contractility.DnMyD88 mice died from heart failure before they were 7 months old,as shown by Kaplan-Meier analysis.Additionally,the heart failure phenotype of dnMyD88 mice was associated with abnormal activation of the Akt/GSK-3β signaling pathway.These data provide the first evidence that normal MyD88 signaling is crucial for maintaining the physiological function of the adult heart.展开更多
基金Supported by Agricultural Science and Technology Innovation Project of Jiangsu Province[CX(16)1006]Fundamental Research Fund of Jiangsu Academy of Agricultural Sciences[ZX(15)3003]
文摘Suzhong swine is a hybrid breed derived from Taihu sows and Landraee boars. To identify Suzhong swine individuals and trace the source of pork products, single nucleotide polymorphisms （SNPs） identification of Suzhong swine individuals was studied. A total of 29 pairs of primers were designed and sev- en pairs of primers were used for identification of Suzhong swine individuals. The products amplified by seven pairs of primers could be directly sequenced, with clean sequencing map background and no ambiguity in sequence read. Totally 52 SNPs loci were amplified by seven pairs of primers, and 41 SNPs loci were reserved for identification of Suzhong swine individuals through correlation analysis and heterezygosity filtration （ H ≥0.1 ）. Meantime, the digital barcodes for SNP identification of 96 individuals of Suzhong swine derived from seven boars and 12 sows were developed, which well distinguished 96 individuals of Suzhong swine. Theoretically, 41SNPs amplified by seven pairs of primers could be used for identification of 5.0 × 10^6 pig individuals. Therefore, digital barcode devel- opment method for SNP identification of Suzbong swine individuals can be used for individual identification of Suzhong swine in scale pig farm and meat product traceability.
基金supported in part by the National Natural Science Foundation of China (30825024)the Ministry of Science and Technology of China (2006BAI23B00,2005CB522501 and 2006CB943500)
文摘Myeloid differentiation protein-88(MyD88) is a crucial adaptor protein in the innate immune response.A protective role for MyD88 in normal cardiac function has been proposed in a surgical hypertrophic model.To assess the in vivo role of MyD88 in cardiac remodeling,we generated transgenic mice with cardiac-restricted expression of a dominant negative mutant of MyD88(dnMyD88).Surprisingly,dnMyD88 transgenic mice displayed characteristic features of heart failure;including heart weight increase,cardiomyocytes enlargement,interstitial fibrosis,and re-expression of "fetal" genes.Echocardiographic examination of dnMyD88 hearts revealed dilated chamber volume and reduced cardiac contractility.DnMyD88 mice died from heart failure before they were 7 months old,as shown by Kaplan-Meier analysis.Additionally,the heart failure phenotype of dnMyD88 mice was associated with abnormal activation of the Akt/GSK-3β signaling pathway.These data provide the first evidence that normal MyD88 signaling is crucial for maintaining the physiological function of the adult heart.
