Construction of the ecological compensation mechanism is an important approach to put the“Beijing-Tianjin-Hebei Coordinated Development Plan”into practice and improve the ecological environment of the Beijing-Tianji...Construction of the ecological compensation mechanism is an important approach to put the“Beijing-Tianjin-Hebei Coordinated Development Plan”into practice and improve the ecological environment of the Beijing-Tianjin-Hebei region.This paper constructs an ecological compensation mechanism for the Beijing-Tianjin-Hebei region based on the footprint balance and footprint deficit after clarifying ecological governance objectives.First,this paper proposes to establish a uniform,hierarchical and classified supply mechanism of ecosystem services according to the classification of land resources,water resources and forest resources for the Beijing-Tianjin-Hebei region.Then,the“Authority with Corresponding Responsibility”for the supply of ecosystem services in the Beijing-Tianjin-Hebei region can be realized through a footprint balance and footprint deficit indicator circulation mechanism.Finally,the scientific and rational ecological compensation standard can be guaranteed through establishing the integrated governance mechanism for ecological compensation in the Beijing-Tianjin-Hebei region and improving the“ecological compensation standard”.The results of this paper can provide sound theoretical support for effectively promoting the improvement of ecosystem services and human well-being in the Beijing-Tianjin-Hebei region.展开更多
In order to obtain pure enzyme with high activity,two amylase producing strains were isolated from soil samples,and named as the strains LZ-10 and LZ-11.According to morphologic observation,physiology and biochemistry...In order to obtain pure enzyme with high activity,two amylase producing strains were isolated from soil samples,and named as the strains LZ-10 and LZ-11.According to morphologic observation,physiology and biochemistry experiments,16S rRNA and gyrB gene analysis,the strains LZ-10 and LZ-11 were identified as Bacillus subtilis.Adopted the method of ammonium sulfate,DEAE-52 anion purify enzyme,finally used polyacrylamide gel electrophoresis(SDS-PAGE)to detect molecular weight.The strain LZ-10 had an amylase activity of 123.3 U·mL^(-1),a purification factor of 6.8,a recovery rate of 69.5% and an optimal temperature of 50℃.The amylase activity of the strain LZ-11 was 59.91 U·mL^(-1),the purification factor was 4.5,the recovery rate was 60.5%,and the optimum temperature was 55℃.The commodity enzyme derived from Bacillus subtilis was 37.5 U·mL^(-1).The relative molecular weight of amylase activity from the two strains was 55 ku.Both thermal stability and pH stability were higher than those of commercialized amylase.展开更多
基金This research is supporteded by the Major Project of Humanities and Social Science Research of the Hebei Education Department(Grant No.ZD201907)the Young Scientists Fund of the Hebei College Science and Technology Research Program(Grant No.QN2018252)the Young Scientists Fund of the National Natural Science Foundation of China(Grant Nos..51909052,41807169).
文摘Construction of the ecological compensation mechanism is an important approach to put the“Beijing-Tianjin-Hebei Coordinated Development Plan”into practice and improve the ecological environment of the Beijing-Tianjin-Hebei region.This paper constructs an ecological compensation mechanism for the Beijing-Tianjin-Hebei region based on the footprint balance and footprint deficit after clarifying ecological governance objectives.First,this paper proposes to establish a uniform,hierarchical and classified supply mechanism of ecosystem services according to the classification of land resources,water resources and forest resources for the Beijing-Tianjin-Hebei region.Then,the“Authority with Corresponding Responsibility”for the supply of ecosystem services in the Beijing-Tianjin-Hebei region can be realized through a footprint balance and footprint deficit indicator circulation mechanism.Finally,the scientific and rational ecological compensation standard can be guaranteed through establishing the integrated governance mechanism for ecological compensation in the Beijing-Tianjin-Hebei region and improving the“ecological compensation standard”.The results of this paper can provide sound theoretical support for effectively promoting the improvement of ecosystem services and human well-being in the Beijing-Tianjin-Hebei region.
基金Supported by Young Doctor Fund of Gansu Education Department,China(2021QB-034)。
文摘In order to obtain pure enzyme with high activity,two amylase producing strains were isolated from soil samples,and named as the strains LZ-10 and LZ-11.According to morphologic observation,physiology and biochemistry experiments,16S rRNA and gyrB gene analysis,the strains LZ-10 and LZ-11 were identified as Bacillus subtilis.Adopted the method of ammonium sulfate,DEAE-52 anion purify enzyme,finally used polyacrylamide gel electrophoresis(SDS-PAGE)to detect molecular weight.The strain LZ-10 had an amylase activity of 123.3 U·mL^(-1),a purification factor of 6.8,a recovery rate of 69.5% and an optimal temperature of 50℃.The amylase activity of the strain LZ-11 was 59.91 U·mL^(-1),the purification factor was 4.5,the recovery rate was 60.5%,and the optimum temperature was 55℃.The commodity enzyme derived from Bacillus subtilis was 37.5 U·mL^(-1).The relative molecular weight of amylase activity from the two strains was 55 ku.Both thermal stability and pH stability were higher than those of commercialized amylase.
基金supported by the natural Science Foundation of hubei Province(no.2015CFB288)hubei Province health and Family Planning Scientific Research Project,China(No.WJ2015MB171)
