In order to determine the physiological mechanism of drought resistance of northern wheat in China,six drought resistant wheat and one sensitivity to drought wheat were planted in pots.They were subjected to drought t...In order to determine the physiological mechanism of drought resistance of northern wheat in China,six drought resistant wheat and one sensitivity to drought wheat were planted in pots.They were subjected to drought treatment and normal water when the plants grew to the 3-leaf stage.Samples were collected at 10,20,30,and 40 days after the drought treatment,respectively.The electrical conductivity,photosynthetic parameters,chlorophyll fluorescence parameters,sugar content,proline content,protein content,and active oxygen scavenging enzyme activity of the plants were detected,and the agronomic traits of the wheat varieties were investigated at maturity.The results indicated that the phenotype and yield-related factors of Darkhan 144 changed little under the drought stress.The relative electrical conductivity of Kefeng 6 and Darkhan 166 was lower under the drought stress,and their cell membrane was less damaged.The Darkhan 144 and Darkhan 166 had higher drought resistance coefficients,and were the wheat varieties with stronger drought resistance.However,the physiological mechanisms of drought resistance of these three wheat were different:Darkhan 144 maintained a higher photosynthetic activity under the drought stress;Darkhan 166 maintained a higher protein content,photosynthetic activity and active oxygen scavenging enzyme activity.In addition,other drought-resistant varieties Kefeng 6,Kefeng 10 and Longmai 26 had a higher content of osmoregulatory substances under the drought stress.展开更多
目的:分离纯化铁皮石斛多糖(DOP),得到纯化后的铁皮石斛多糖DOP-1,并以秀丽隐杆线虫为模式生物,研究DOP-1的抗衰老作用。方法:以铁皮石斛鲜品为原料,经提取、浓缩、乙醇沉淀和真空干燥后,得到铁皮石斛粗多糖,铁皮石斛粗多糖经DEAE-Sepha...目的:分离纯化铁皮石斛多糖(DOP),得到纯化后的铁皮石斛多糖DOP-1,并以秀丽隐杆线虫为模式生物,研究DOP-1的抗衰老作用。方法:以铁皮石斛鲜品为原料,经提取、浓缩、乙醇沉淀和真空干燥后,得到铁皮石斛粗多糖,铁皮石斛粗多糖经DEAE-Sepharose Fast Flow层析柱和HW-55F凝胶柱洗脱后获得纯品铁皮石斛多糖DOP-1,通过高效凝胶渗透色谱法测定其纯度及相对分子质量等参数,同时研究不同浓度DOP-1(100μg/mL、200μg/mL、400μg/mL)对秀丽隐杆线虫寿命、生殖能力、抗高温损伤、氧化应激损能力及脂褐素的影响。结果:分离纯化后的DOP-1为均一性多糖,其相对分子质量为3.260×10^(5),不同浓度DOP-1均可以显著延长秀丽隐杆线虫的寿命(P<0.05或P<0.01),其中以200μg/mL DOP-1作用最为明显,与对照组比较,寿命延长了43.37%,DOP-1对高温损伤和氧化应激损伤有一定的抵御作用,能有效降低秀丽隐杆线虫体内脂褐素的积累,并对其生殖能力无明显影响。结论:分离纯化后的DOP-1为均一性多糖,并具有较显著的抗衰老作用,可对其结构与抗衰老活性之间关系的研究提供参考。展开更多
基金the National Ministry of Science and Technology Key Project(2018YFE0123300)the National Modern Agricultural Wheat Industry Technology System Keshan Comprehensive Test Station(CARS‒03‒54)the Collaborative Innovation and Extension System of Modern Agricultural Wheat in Heilongjiang Province。
文摘In order to determine the physiological mechanism of drought resistance of northern wheat in China,six drought resistant wheat and one sensitivity to drought wheat were planted in pots.They were subjected to drought treatment and normal water when the plants grew to the 3-leaf stage.Samples were collected at 10,20,30,and 40 days after the drought treatment,respectively.The electrical conductivity,photosynthetic parameters,chlorophyll fluorescence parameters,sugar content,proline content,protein content,and active oxygen scavenging enzyme activity of the plants were detected,and the agronomic traits of the wheat varieties were investigated at maturity.The results indicated that the phenotype and yield-related factors of Darkhan 144 changed little under the drought stress.The relative electrical conductivity of Kefeng 6 and Darkhan 166 was lower under the drought stress,and their cell membrane was less damaged.The Darkhan 144 and Darkhan 166 had higher drought resistance coefficients,and were the wheat varieties with stronger drought resistance.However,the physiological mechanisms of drought resistance of these three wheat were different:Darkhan 144 maintained a higher photosynthetic activity under the drought stress;Darkhan 166 maintained a higher protein content,photosynthetic activity and active oxygen scavenging enzyme activity.In addition,other drought-resistant varieties Kefeng 6,Kefeng 10 and Longmai 26 had a higher content of osmoregulatory substances under the drought stress.
文摘目的:分离纯化铁皮石斛多糖(DOP),得到纯化后的铁皮石斛多糖DOP-1,并以秀丽隐杆线虫为模式生物,研究DOP-1的抗衰老作用。方法:以铁皮石斛鲜品为原料,经提取、浓缩、乙醇沉淀和真空干燥后,得到铁皮石斛粗多糖,铁皮石斛粗多糖经DEAE-Sepharose Fast Flow层析柱和HW-55F凝胶柱洗脱后获得纯品铁皮石斛多糖DOP-1,通过高效凝胶渗透色谱法测定其纯度及相对分子质量等参数,同时研究不同浓度DOP-1(100μg/mL、200μg/mL、400μg/mL)对秀丽隐杆线虫寿命、生殖能力、抗高温损伤、氧化应激损能力及脂褐素的影响。结果:分离纯化后的DOP-1为均一性多糖,其相对分子质量为3.260×10^(5),不同浓度DOP-1均可以显著延长秀丽隐杆线虫的寿命(P<0.05或P<0.01),其中以200μg/mL DOP-1作用最为明显,与对照组比较,寿命延长了43.37%,DOP-1对高温损伤和氧化应激损伤有一定的抵御作用,能有效降低秀丽隐杆线虫体内脂褐素的积累,并对其生殖能力无明显影响。结论:分离纯化后的DOP-1为均一性多糖,并具有较显著的抗衰老作用,可对其结构与抗衰老活性之间关系的研究提供参考。
