The anterior auditory field(AAF)is a core region of the auditory cortex and plays a vital role in discrimination tasks.However,the role of the AAF corticostriatal neurons in frequency discrimination remains unclear.He...The anterior auditory field(AAF)is a core region of the auditory cortex and plays a vital role in discrimination tasks.However,the role of the AAF corticostriatal neurons in frequency discrimination remains unclear.Here,we used c-Fos staining,fiber photometry recording,and pharmacogenetic manipulation to investigate the function of the AAF corticostriatal neurons in a frequency discrimination task.c-Fos staining and fiber photometry recording revealed that the activity of AAF pyramidal neurons was significantly elevated during the frequency discrimination task.Pharmacogenetic inhibition of AAF pyramidal neurons significantly impaired frequency discrimination.In addition,histological results revealed that AAF pyramidal neurons send strong projections to the striatum.Moreover,pharmacogenetic suppression of the striatal projections from pyramidal neurons in the AAF significantly disrupted the frequency discrimination.Collectively,our findings show that AAF pyramidal neurons,particularly the AAF–striatum projections,play a crucial role in frequency discrimination behavior.展开更多
Herein a facile and controllable heterocoagulation between polystyrene （PS） microspheres and multiwalled carbon nanotubes （MWCNTs） is introduced based on colloid thermodynamics. The MWCNTs play the role of steric ...Herein a facile and controllable heterocoagulation between polystyrene （PS） microspheres and multiwalled carbon nanotubes （MWCNTs） is introduced based on colloid thermodynamics. The MWCNTs play the role of steric stabilizer for stabilizing the metastable PS microspheres and thus immobilize spontaneously on the surface of PS microspheres. The synthesized MWCNTs-coated PS composite particles have been extensively characterized by scanning electron microscopy, transmission electron microscopy, thermogravimetry and Raman spectroscopy. The results indicate that the structure and morphology of the resultant MWCNTs-coated PS composite particles are significantly affected by the weight ratio of PS and MWNCTs and the amount of poly（vinylpyrrolidone） that is injected into PS dispersion before they are mixed with MWCNTs. Therefore, these composite particles have the potential to produce MWCNTs-based composite materials with controllable mass loading and dispersity of MWCNTs.展开更多
基金supported by grants from the National Natural Science Foundation of China(31871075 and 32071015)the Open Project of Chongqing Key Laboratory of Neurobiology(cqsjsw202103 and cqsjsw202101)the Natural Science Foundation of Chongqing(cstc2020jcyj-msxmX0391).
文摘The anterior auditory field(AAF)is a core region of the auditory cortex and plays a vital role in discrimination tasks.However,the role of the AAF corticostriatal neurons in frequency discrimination remains unclear.Here,we used c-Fos staining,fiber photometry recording,and pharmacogenetic manipulation to investigate the function of the AAF corticostriatal neurons in a frequency discrimination task.c-Fos staining and fiber photometry recording revealed that the activity of AAF pyramidal neurons was significantly elevated during the frequency discrimination task.Pharmacogenetic inhibition of AAF pyramidal neurons significantly impaired frequency discrimination.In addition,histological results revealed that AAF pyramidal neurons send strong projections to the striatum.Moreover,pharmacogenetic suppression of the striatal projections from pyramidal neurons in the AAF significantly disrupted the frequency discrimination.Collectively,our findings show that AAF pyramidal neurons,particularly the AAF–striatum projections,play a crucial role in frequency discrimination behavior.
基金financially supported by the National Natural Science Foundation of China(Nos.21204030 and 51133002)the Fundamental Research Funds for the Central Universities(JUSRP111A05)
文摘Herein a facile and controllable heterocoagulation between polystyrene （PS） microspheres and multiwalled carbon nanotubes （MWCNTs） is introduced based on colloid thermodynamics. The MWCNTs play the role of steric stabilizer for stabilizing the metastable PS microspheres and thus immobilize spontaneously on the surface of PS microspheres. The synthesized MWCNTs-coated PS composite particles have been extensively characterized by scanning electron microscopy, transmission electron microscopy, thermogravimetry and Raman spectroscopy. The results indicate that the structure and morphology of the resultant MWCNTs-coated PS composite particles are significantly affected by the weight ratio of PS and MWNCTs and the amount of poly（vinylpyrrolidone） that is injected into PS dispersion before they are mixed with MWCNTs. Therefore, these composite particles have the potential to produce MWCNTs-based composite materials with controllable mass loading and dispersity of MWCNTs.
