DEAR EDITOR,Osteonecrosis is a common human disease in orthopedics.It is difficult to treat,and half of patients may need artificial joint replacement,resulting in a considerable economic burden and a reduction in qua...DEAR EDITOR,Osteonecrosis is a common human disease in orthopedics.It is difficult to treat,and half of patients may need artificial joint replacement,resulting in a considerable economic burden and a reduction in quality of life.Hormones are one of the major causes of osteonecrosis and high doses of corticosteroids are considered the most dangerous factor.展开更多
In flowering plants, pollen tube growth is essential for delivery of male gametes into the female gametophyte or embryo sac for double fertilization. Although many genes have been identified as being involved in the p...In flowering plants, pollen tube growth is essential for delivery of male gametes into the female gametophyte or embryo sac for double fertilization. Although many genes have been identified as being involved in the process, the molecular mechanisms of pollen tube growth remains poorly understood. in this study, we identified that the Arabidopsis Transmembrane Protein 18 （AtTMEM18） gene played important roles in pollen tube growth. The AtTMEM18 shares a high similarity with the Transmembrane 18 proteins （TMEM18s） that are conserved in most eukaryotes and may play important roles in obesity in humans. Mutation in the AtTMEM18 by a Ds insertion caused abnormal callose deposition in the pollen grains and had a significant impact on pollen germination and pollen tube growth. AtTMEM18 is expressed in pollen grains, pollen tubes, root tips and other vegetative tissues. The pollen-rescued assays showed that the mutation in AtTMEM18 also caused defects in roots, stems, leaves and transmitting tracts. AtTMEM18-GFP was located around the nuclei. Genetic assays demonstrated that the localization of AtTMEM18 around the nuclei in the generative cells of pollen grains was essential for the male fertility.Furthermore, expression of the rice TM EM18-homologous protein （OsTMEM18） driven by LAT52 promoter could recover the fertility of the Arabidopsis attmem18 mutant. These results suggested that the TMEM18 is important for plant growth in Arabidopsis.展开更多
基金supported by the National Science and Key Technology Support Program(2014BAI01B01-07)Science and Technology Key Projects of Kunming(2014-04-A-S-01-3072)。
文摘DEAR EDITOR,Osteonecrosis is a common human disease in orthopedics.It is difficult to treat,and half of patients may need artificial joint replacement,resulting in a considerable economic burden and a reduction in quality of life.Hormones are one of the major causes of osteonecrosis and high doses of corticosteroids are considered the most dangerous factor.
基金supported by the research grants from the Ministry of Sciences and Technology(973 project number:2007CB108700)the Natural Science Foundation of China(NSFC,project number:30530060)+1 种基金the Ministry of Education(111 project numbered B06003)the BJAST Youth Backbone Training Plan(No.2015-24)
文摘In flowering plants, pollen tube growth is essential for delivery of male gametes into the female gametophyte or embryo sac for double fertilization. Although many genes have been identified as being involved in the process, the molecular mechanisms of pollen tube growth remains poorly understood. in this study, we identified that the Arabidopsis Transmembrane Protein 18 （AtTMEM18） gene played important roles in pollen tube growth. The AtTMEM18 shares a high similarity with the Transmembrane 18 proteins （TMEM18s） that are conserved in most eukaryotes and may play important roles in obesity in humans. Mutation in the AtTMEM18 by a Ds insertion caused abnormal callose deposition in the pollen grains and had a significant impact on pollen germination and pollen tube growth. AtTMEM18 is expressed in pollen grains, pollen tubes, root tips and other vegetative tissues. The pollen-rescued assays showed that the mutation in AtTMEM18 also caused defects in roots, stems, leaves and transmitting tracts. AtTMEM18-GFP was located around the nuclei. Genetic assays demonstrated that the localization of AtTMEM18 around the nuclei in the generative cells of pollen grains was essential for the male fertility.Furthermore, expression of the rice TM EM18-homologous protein （OsTMEM18） driven by LAT52 promoter could recover the fertility of the Arabidopsis attmem18 mutant. These results suggested that the TMEM18 is important for plant growth in Arabidopsis.
