Brain-derived neurotrophic factor(BDNF)has robust effects on synaptogenesis,neuronal differentiation and synaptic transmission and plasticity.The maturation of BDNF is a complex process.Proprotein convertase 1/3(PC1/3...Brain-derived neurotrophic factor(BDNF)has robust effects on synaptogenesis,neuronal differentiation and synaptic transmission and plasticity.The maturation of BDNF is a complex process.Proprotein convertase 1/3(PC1/3)has a key role in the cleavage of protein precursors that are directed to regulated secretory pathways;however,it is not clear whether PC1/3 mediates the change in BDNF levels caused by ischemia.To clarify the role of PC1/3 in BDNF maturation in ischemic cortical neurons,primary cortical neurons from fetal rats were cultured in a humidified environment of 95%N_2 and 5%CO_2 in a glucose-free Dulbecco's modified Eagle's medium at 37℃for3 hours.Enzyme-linked immunosorbent assays and western blotting showed that after oxygen-glucose deprivation,the secreted and intracellular levels of BDNF were significantly reduced and the intracellular level of PC1/3 was decreased.Transient transfection of cortical neurons with a PC1/3 overexpression plasmid followed by oxygen-glucose deprivation resulted in increased PC1/3 levels and increased BDNF levels.When levels of the BDNF precursor protein were reduced,the concentration of BDNF in the culture medium was increased.These results indicate that PC 1/3 cleavage of BDNF is critical for the conversion of pro-BDNF in rat cortical neurons during ischemia.The study was approved by the Animal Ethics Committee of Wuhan University School of Basic Medical Sciences.展开更多
Objectives:To observe the effects of transitional care on the quality of life of chronic obstructive pulmonary disease(COPD)patients.Methods:A total of 114 COPD patients were recruited from the First Affiliated Hospit...Objectives:To observe the effects of transitional care on the quality of life of chronic obstructive pulmonary disease(COPD)patients.Methods:A total of 114 COPD patients were recruited from the First Affiliated Hospital,Sun Yat-sen University,Guangzhou,China and divided equally into an intervention group and control group.Following discharge,patients from the intervention group recieved threemonths intervention in addition to regular nursing care,while control group patients received regular nursing care only.Patients’quality of life was measured using the St.George’s respiratory questionnaire(SGRQ),the 12-item General Health Questionnaire(GHQ-12)and body mass index(BMI).Results:The symptoms section score,the activity section score,the impacts section score,the total score and the rate of mental disorders were significantly changed after the intervention while there was no statistical difference in BMI between groups.Conclusions:Transitional care can improve health-related quality of life in COPD patients who have recently suffered an exacerbation.展开更多
The G2019S mutation of the leucine-rich repeat kinase 2(LRRK2)is the most common genetic cause of Parkinson's disease (PD).However,the molecular mechanisms of LRRK2 mutation contributing to the onset and progressi...The G2019S mutation of the leucine-rich repeat kinase 2(LRRK2)is the most common genetic cause of Parkinson's disease (PD).However,the molecular mechanisms of LRRK2 mutation contributing to the onset and progression of PD have not been fully illustrated.We generated HEK293 cells stably transfected with α-synuclein and investigated the effect of LRRK2 G2019S mutation on the degradation of α-synuclein.The lysosomal activity was assessed by the protein degradation of glyceraldehyde-3-phosphate dehydrogenase and ribonuclease A.It was found that α-synuclein was mainly degraded in lysosomes.LRRK2G2019S inhibited the degradation of α-synuclein,and promoted its aggregation.LRRK2G 2019S also decreased the activities of lysosomal enzymes including cathepsin B and cathepsin L.Furthermore,the inhibitory effect of LRRK2 G2019S on lysosomal functions did not depend on its kinase activity.These findings indicated that the inhibitory effect of LRRK2 G2019S on α-synuclein degradation could underlie the pathogenesis of aberrant α-synuclein aggregation in PD with LRRK2 mutation.展开更多
H13 tool steel was successfully prepared by selective laser melting(SLM)technology.The effects of heat treatment on the microstructure,mechanical properties,and tribological properties of SLMed H13 steel were investig...H13 tool steel was successfully prepared by selective laser melting(SLM)technology.The effects of heat treatment on the microstructure,mechanical properties,and tribological properties of SLMed H13 steel were investigated.The heat treatment process involved a solution treatment and a double aging treatment of the deposited H13 tool steel prepared by SLM.The aim is to optimize the microstructure and mechanical properties of SLMed H13 steel.Due to the rapid heating and cooling effects of SLM,carbide precipitation in the deposited H13 steel was not uniform and residual stresses were present.The purpose of the solution treatment is to dissolve the solution at a high temperature to eliminate the residual stresses and defects introduced by the SLM-forming structure.The solution treatment and first aging treatment produced the precipitation of small carbides at the grain boundaries and inside the crystals,which increased the hardness of SLMed H13 steel.The hardness increased from 538±4.0 HV of the as-deposited sample to 548±5.8 HV of samples after the first aging treatment.Accordingly,the ultimate tensile strength and the elongation at break decreased from 1882 MPa and 11.5%in the as-deposited sample to 1697 MPa and 7.9%in those after the first aging treatment,respectively.Furthermore,the friction coefficient and wear rate in the as-deposited sample decreased from 0.5160 and 2.36×10^(–6)mm^(−3)N^(−1)m^(−1)to 0.4244 and 1.04×10^(–6)mm^(−3)N^(−1)m^(−1),respectively.However,the distribution of carbides inside the crystals was not uniform.The second aging treatment adjusted the morphology of carbide precipitation and made it more uniform,but the precipitation of carbides grew and settled at the bottom of the grain boundaries.The hardness decreased to 533±6.7 HV compared with that with the first aging treatment,but the ultimate tensile strength and plasticity reached a balance(1807 MPa,14.05%).Accordingly,the friction coefficient and wear rate also showed a stable and decreasing trend(0.4407,0.98×10^(–6)mm^(−3)N^(−1)m^(−1)).展开更多
Development of multifunctional and high-performance silicone aerogel is highly required for various promising applications.However,unstable cross-linking structure and poor thermal stability of silicone network as wel...Development of multifunctional and high-performance silicone aerogel is highly required for various promising applications.However,unstable cross-linking structure and poor thermal stability of silicone network as well as complicated processing restrict the practical use significantly.Herein,we report a facile and versatile ambient drying strategy to fabricate lightweight,wide-temperature flexible,super-hydrophobic and flame retardant silicone composite aerogels modified with low-content functionalized graphene oxide(FGO).After optimizing silane molecules,incorporation ofγ-aminopropyltriethoxysilane functionalization is found to promote the dispersion stability of GO during the hydrolysis-polymerization process and thus produce the formation of unique strip-like co-cross-linked network.Consequently,the aerogels containing∼2.0 wt%FGO not only possess good cyclic compressive stability under strain of 70%for 100 cycles and outstanding mechanical reliability in wide temperature range(from liquid nitrogen to 350℃),but also display excellent flame resistance and super-hydrophobicity.Further,the optimized silicone/FGO aerogels display exceptional thermal insulating performance superior to pure aerogel and hydrocarbon polymer foams,and they also show efficient oil absorption and separation capacity for var-ious solvents and oil from water.Clearly,this work provides a new route for the rational design and development of advanced silicone composite aerogels for multifunctional applications.展开更多
Porous titanium fiber materials with the fiber sizes of 70--120 μm in diameter were prepared by vacuum sintering technology. The morphology and compressive properties of porous titanium fiber materials were investiga...Porous titanium fiber materials with the fiber sizes of 70--120 μm in diameter were prepared by vacuum sintering technology. The morphology and compressive properties of porous titanium fiber materials were investigated by using a scanning electron microscope (SEM) and an MST 858 compression testing machine in quasi-static condition. The results show that porous titanium fibers form complex micro-networks. The stress-strain curves of por- ous titanium fiber materials exhibit elastic region, platform region and densification region and no collapse during platform region. The yield strength of porous titanium fiber materials decreases with increasing the porosity and increasing the fiber diameter.展开更多
The porous titanium fiber materials with open porosity were successfully prepared by the vacuum sintering technology. The morphology characteristics of sintering neck of porous titanium fiber materials were investigat...The porous titanium fiber materials with open porosity were successfully prepared by the vacuum sintering technology. The morphology characteristics of sintering neck of porous titanium fiber materials were investigated by scanning electron microscopy (SEM). The results show that the formation and growth of sintering neck of porous ti- tanium fiber material approximately follow the rule that the primary mechanism is grain boundary diffusion and sub- sidiary mechanisms are other diffusion mechanisms during the sintering process. The formation and growth of the sintering neck depend mainly on the sintering temperature and slightly on the soaking time. The sintering system of porous titanium fiber material was determined and the equation of the sintering neck's length was established.展开更多
The effect of hydrogen addition on compression deformation behaviour of Ti-0.3Mo-0.8Ni alloy argon-arc welded joint has been investigated.Evolution mechanism of hydrogen-induced flow stress was discussed in detail.The...The effect of hydrogen addition on compression deformation behaviour of Ti-0.3Mo-0.8Ni alloy argon-arc welded joint has been investigated.Evolution mechanism of hydrogen-induced flow stress was discussed in detail.The results show that with increasing hydrogen content,the stretching and bending extent of fully lamellar microstructures including ot lamellas and acicular hydride continued to increase,the morphology of dynamic recrystallization(DRX)grains tended to change from approximately equiaxed to large lamellar shape,and the quantity of DRX grains and recrystallization degree of grains increased obviously.A large number of dislocations concentrated in the vicinity of the hydride.Steady stress was decreased continuously with increasing hydrogen content,while peak stress of the hydrogenated 0.12 wt.% H weld zone was decreased to the minimum value and then increased slowly.A slight decrease in flow stress of the hydrogenated 0.05 wt.% H weld zone was caused by limited increase in the volume fraction of softer βphase.Hydrogen-induced DRX of a phase and improved dislocation movement by strong interaction between the hydride and dislocation directly resulted in a sharp drop in flow stress of the hydrogenated 0.12 and 0.21 wt.% H weld zone.Solute hydrogen also finitely contributed to a sharp drop in flow stress of the hydrogenated 0.12 and 0.21 wt.% H weld zone by promoted local softening,which induced continuous DRX and more movable dislocations to participate in slipping or climbing.The reinforcement effect and plastic deformation of the hydride and solution strengthening of P phase induced by solute hydrogen finally led to the increase in flow stress of the hydrogenated 0.21 wt.% H weld zone in its true strain range from 0 to 0.36.展开更多
Porous titanium fibre materials with different structural parameters were prepared by vacu- um sintering method. The thickness, porosity and wire diameter of prepared materials were investigated to understand the effe...Porous titanium fibre materials with different structural parameters were prepared by vacu- um sintering method. The thickness, porosity and wire diameter of prepared materials were investigated to understand the effects of structural parameters on pool heat transmission performance of titanium fibre porous material. As a result, better heat transfer performance is obtained when overheating is less than 10 ℃. In addition, when the wire diameter is smal- ler, the heat transfer is better. However, when superheating is above 10 ℃, heat transfer performance can be improved by increasing the wire diameter. Moreover, thickness influ- ences the superficial area of the prepared material and affects the thermal resistance when bubbles move inside the material; superficial area and thermal resistance are the two key factors that jointly impact the heat transfer in relation to the thickness of the materials. Ex- perimental results also show that the materials of 3 mm in thickness exhibit the best per formance for heat transmission. Furthermore, changes in porosity affect the nucleation site density and the resistance to bubble detachment; however, the nucleation site density and the resistance to bubble detachment conflict with each other. In summary, the titanium fi- bre porous material with a 50% porosity exhibits suitable heat transfer performance.展开更多
基金supported by the National Nature Science Foundation of China,No.81501053(to YC)
文摘Brain-derived neurotrophic factor(BDNF)has robust effects on synaptogenesis,neuronal differentiation and synaptic transmission and plasticity.The maturation of BDNF is a complex process.Proprotein convertase 1/3(PC1/3)has a key role in the cleavage of protein precursors that are directed to regulated secretory pathways;however,it is not clear whether PC1/3 mediates the change in BDNF levels caused by ischemia.To clarify the role of PC1/3 in BDNF maturation in ischemic cortical neurons,primary cortical neurons from fetal rats were cultured in a humidified environment of 95%N_2 and 5%CO_2 in a glucose-free Dulbecco's modified Eagle's medium at 37℃for3 hours.Enzyme-linked immunosorbent assays and western blotting showed that after oxygen-glucose deprivation,the secreted and intracellular levels of BDNF were significantly reduced and the intracellular level of PC1/3 was decreased.Transient transfection of cortical neurons with a PC1/3 overexpression plasmid followed by oxygen-glucose deprivation resulted in increased PC1/3 levels and increased BDNF levels.When levels of the BDNF precursor protein were reduced,the concentration of BDNF in the culture medium was increased.These results indicate that PC 1/3 cleavage of BDNF is critical for the conversion of pro-BDNF in rat cortical neurons during ischemia.The study was approved by the Animal Ethics Committee of Wuhan University School of Basic Medical Sciences.
基金This study was supported by Natural Science Foundation of Guangdong Province,China(Project No.07001681).
文摘Objectives:To observe the effects of transitional care on the quality of life of chronic obstructive pulmonary disease(COPD)patients.Methods:A total of 114 COPD patients were recruited from the First Affiliated Hospital,Sun Yat-sen University,Guangzhou,China and divided equally into an intervention group and control group.Following discharge,patients from the intervention group recieved threemonths intervention in addition to regular nursing care,while control group patients received regular nursing care only.Patients’quality of life was measured using the St.George’s respiratory questionnaire(SGRQ),the 12-item General Health Questionnaire(GHQ-12)and body mass index(BMI).Results:The symptoms section score,the activity section score,the impacts section score,the total score and the rate of mental disorders were significantly changed after the intervention while there was no statistical difference in BMI between groups.Conclusions:Transitional care can improve health-related quality of life in COPD patients who have recently suffered an exacerbation.
基金the National Natura Science Foundation of China(NSFC)(No.81401051,No 81671051,and No.81501107).
文摘The G2019S mutation of the leucine-rich repeat kinase 2(LRRK2)is the most common genetic cause of Parkinson's disease (PD).However,the molecular mechanisms of LRRK2 mutation contributing to the onset and progression of PD have not been fully illustrated.We generated HEK293 cells stably transfected with α-synuclein and investigated the effect of LRRK2 G2019S mutation on the degradation of α-synuclein.The lysosomal activity was assessed by the protein degradation of glyceraldehyde-3-phosphate dehydrogenase and ribonuclease A.It was found that α-synuclein was mainly degraded in lysosomes.LRRK2G2019S inhibited the degradation of α-synuclein,and promoted its aggregation.LRRK2G 2019S also decreased the activities of lysosomal enzymes including cathepsin B and cathepsin L.Furthermore,the inhibitory effect of LRRK2 G2019S on lysosomal functions did not depend on its kinase activity.These findings indicated that the inhibitory effect of LRRK2 G2019S on α-synuclein degradation could underlie the pathogenesis of aberrant α-synuclein aggregation in PD with LRRK2 mutation.
基金supported by National Natural Science Foundation of China(Grant No.52104341)Key Technologies Research and Development Program(Grant Nos.2021YFB3701902 and 2021YFB3701903)+1 种基金Natural Science Basic Research Program of Shaanxi Province(Grant Nos.2022JM-259 and 2022JQ-367)Postdoctoral Research Foundation of China(Grant No.2021M702554).
文摘H13 tool steel was successfully prepared by selective laser melting(SLM)technology.The effects of heat treatment on the microstructure,mechanical properties,and tribological properties of SLMed H13 steel were investigated.The heat treatment process involved a solution treatment and a double aging treatment of the deposited H13 tool steel prepared by SLM.The aim is to optimize the microstructure and mechanical properties of SLMed H13 steel.Due to the rapid heating and cooling effects of SLM,carbide precipitation in the deposited H13 steel was not uniform and residual stresses were present.The purpose of the solution treatment is to dissolve the solution at a high temperature to eliminate the residual stresses and defects introduced by the SLM-forming structure.The solution treatment and first aging treatment produced the precipitation of small carbides at the grain boundaries and inside the crystals,which increased the hardness of SLMed H13 steel.The hardness increased from 538±4.0 HV of the as-deposited sample to 548±5.8 HV of samples after the first aging treatment.Accordingly,the ultimate tensile strength and the elongation at break decreased from 1882 MPa and 11.5%in the as-deposited sample to 1697 MPa and 7.9%in those after the first aging treatment,respectively.Furthermore,the friction coefficient and wear rate in the as-deposited sample decreased from 0.5160 and 2.36×10^(–6)mm^(−3)N^(−1)m^(−1)to 0.4244 and 1.04×10^(–6)mm^(−3)N^(−1)m^(−1),respectively.However,the distribution of carbides inside the crystals was not uniform.The second aging treatment adjusted the morphology of carbide precipitation and made it more uniform,but the precipitation of carbides grew and settled at the bottom of the grain boundaries.The hardness decreased to 533±6.7 HV compared with that with the first aging treatment,but the ultimate tensile strength and plasticity reached a balance(1807 MPa,14.05%).Accordingly,the friction coefficient and wear rate also showed a stable and decreasing trend(0.4407,0.98×10^(–6)mm^(−3)N^(−1)m^(−1)).
基金financially supported by the National Natural Science Foundation of China (Nos. 51973047 and 12002112)the Science Foundation and Technology Project of Zhejiang Province (No. Z22E035302)+1 种基金the Science Foundation and Technology Project of Shandong Province (No. ZR2020LFG004)the Project for Science and Technology Program of Hangzhou (Nos. 20191203B16 and 20201203B136)
文摘Development of multifunctional and high-performance silicone aerogel is highly required for various promising applications.However,unstable cross-linking structure and poor thermal stability of silicone network as well as complicated processing restrict the practical use significantly.Herein,we report a facile and versatile ambient drying strategy to fabricate lightweight,wide-temperature flexible,super-hydrophobic and flame retardant silicone composite aerogels modified with low-content functionalized graphene oxide(FGO).After optimizing silane molecules,incorporation ofγ-aminopropyltriethoxysilane functionalization is found to promote the dispersion stability of GO during the hydrolysis-polymerization process and thus produce the formation of unique strip-like co-cross-linked network.Consequently,the aerogels containing∼2.0 wt%FGO not only possess good cyclic compressive stability under strain of 70%for 100 cycles and outstanding mechanical reliability in wide temperature range(from liquid nitrogen to 350℃),but also display excellent flame resistance and super-hydrophobicity.Further,the optimized silicone/FGO aerogels display exceptional thermal insulating performance superior to pure aerogel and hydrocarbon polymer foams,and they also show efficient oil absorption and separation capacity for var-ious solvents and oil from water.Clearly,this work provides a new route for the rational design and development of advanced silicone composite aerogels for multifunctional applications.
基金Item Sponsored by National Natural Science Foundation of China(51304153)Natural Science Foundation of Shaanxi Province of China(2012JM6017)
文摘Porous titanium fiber materials with the fiber sizes of 70--120 μm in diameter were prepared by vacuum sintering technology. The morphology and compressive properties of porous titanium fiber materials were investigated by using a scanning electron microscope (SEM) and an MST 858 compression testing machine in quasi-static condition. The results show that porous titanium fibers form complex micro-networks. The stress-strain curves of por- ous titanium fiber materials exhibit elastic region, platform region and densification region and no collapse during platform region. The yield strength of porous titanium fiber materials decreases with increasing the porosity and increasing the fiber diameter.
基金Item Sponsored by National Natural Science Foundation of China(51304153)Natural Science Foundation of Shaanxi Province of China(2012JM6017)
文摘The porous titanium fiber materials with open porosity were successfully prepared by the vacuum sintering technology. The morphology characteristics of sintering neck of porous titanium fiber materials were investigated by scanning electron microscopy (SEM). The results show that the formation and growth of sintering neck of porous ti- tanium fiber material approximately follow the rule that the primary mechanism is grain boundary diffusion and sub- sidiary mechanisms are other diffusion mechanisms during the sintering process. The formation and growth of the sintering neck depend mainly on the sintering temperature and slightly on the soaking time. The sintering system of porous titanium fiber material was determined and the equation of the sintering neck's length was established.
基金The authors would like to gratefully acknowledge that this work was supported by the China Postdoctoral Science Foundation(Grant No.2020M672306)National Natural Science Foundation of China(Grant Nos.51874225 and 51671152).
文摘The effect of hydrogen addition on compression deformation behaviour of Ti-0.3Mo-0.8Ni alloy argon-arc welded joint has been investigated.Evolution mechanism of hydrogen-induced flow stress was discussed in detail.The results show that with increasing hydrogen content,the stretching and bending extent of fully lamellar microstructures including ot lamellas and acicular hydride continued to increase,the morphology of dynamic recrystallization(DRX)grains tended to change from approximately equiaxed to large lamellar shape,and the quantity of DRX grains and recrystallization degree of grains increased obviously.A large number of dislocations concentrated in the vicinity of the hydride.Steady stress was decreased continuously with increasing hydrogen content,while peak stress of the hydrogenated 0.12 wt.% H weld zone was decreased to the minimum value and then increased slowly.A slight decrease in flow stress of the hydrogenated 0.05 wt.% H weld zone was caused by limited increase in the volume fraction of softer βphase.Hydrogen-induced DRX of a phase and improved dislocation movement by strong interaction between the hydride and dislocation directly resulted in a sharp drop in flow stress of the hydrogenated 0.12 and 0.21 wt.% H weld zone.Solute hydrogen also finitely contributed to a sharp drop in flow stress of the hydrogenated 0.12 and 0.21 wt.% H weld zone by promoted local softening,which induced continuous DRX and more movable dislocations to participate in slipping or climbing.The reinforcement effect and plastic deformation of the hydride and solution strengthening of P phase induced by solute hydrogen finally led to the increase in flow stress of the hydrogenated 0.21 wt.% H weld zone in its true strain range from 0 to 0.36.
基金supported by the National Natural Science Foundation of China(Grant No.51671152 and No.51304153)State Key Laboratory of Powder Metallurgy,Central South University,Changsha,China
文摘Porous titanium fibre materials with different structural parameters were prepared by vacu- um sintering method. The thickness, porosity and wire diameter of prepared materials were investigated to understand the effects of structural parameters on pool heat transmission performance of titanium fibre porous material. As a result, better heat transfer performance is obtained when overheating is less than 10 ℃. In addition, when the wire diameter is smal- ler, the heat transfer is better. However, when superheating is above 10 ℃, heat transfer performance can be improved by increasing the wire diameter. Moreover, thickness influ- ences the superficial area of the prepared material and affects the thermal resistance when bubbles move inside the material; superficial area and thermal resistance are the two key factors that jointly impact the heat transfer in relation to the thickness of the materials. Ex- perimental results also show that the materials of 3 mm in thickness exhibit the best per formance for heat transmission. Furthermore, changes in porosity affect the nucleation site density and the resistance to bubble detachment; however, the nucleation site density and the resistance to bubble detachment conflict with each other. In summary, the titanium fi- bre porous material with a 50% porosity exhibits suitable heat transfer performance.