背景急性出血坏死型胰腺炎是临床常见的消化系统急症,肺损伤是其常见并发症和致死原因.黄芪注射液具有调节内分泌、增强免疫力、抑制病毒、抗炎、抗氧化应激等作用,但目前尚未见关于黄芪注射液在重症急性胰腺炎肺损伤中的相关研究.目的...背景急性出血坏死型胰腺炎是临床常见的消化系统急症,肺损伤是其常见并发症和致死原因.黄芪注射液具有调节内分泌、增强免疫力、抑制病毒、抗炎、抗氧化应激等作用,但目前尚未见关于黄芪注射液在重症急性胰腺炎肺损伤中的相关研究.目的分析黄芪注射液通过调控雷帕霉素靶蛋白(mammalian target of rapamycin,mTOR)/核糖体70S小亚基S6蛋白激酶(ribosome 70S small subunit S6 protein kinase,p70S6K)信号通路对急性出血坏死型胰腺炎(acute hemorrhagic necrotic pancreatitis,AHNP)肺损伤炎症反应的影响.方法48只成年SD大鼠简单随机化分为对照组、模型组、低、中、高黄芪注射液组、阳性对照组,各8只,记录各组胰腺与肺组织组织病理学变化,比较各组Schmidt评分、血清淀粉酶(amylase,AMS)Hofouaer评分、动脉血二氧化碳分压(PaCO_(2))、动脉血氧分压(PaO_(2))、炎症因子[肿瘤坏死因子-α(tumor necrosis factor-alpha,TNF-α)、白介素-6(interleukin-6,IL-6)]、mTOR/p70S6K信号通路蛋白[mTOR、p70S6K、p-p70S6K、p-mTOR、真核启动因子4E结合蛋白1(p-4E binding protein 1,4EBP1)]水平.结果对照组胰腺、肺组织无异常变化;模型组胰腺实质呈片状坏死、充血水肿,胰腺腺泡细胞与间质细胞大量破坏,小叶结构紊乱,大量炎症细胞浸润与红细胞溢出,肺泡结构排卵紊乱、间质水肿、炎性细胞浸润、肺泡间隔增宽、灶性或片状肺不张、出血;低、中、高黄芪注射液组胰腺与模型组相比,呈现出不同程度改善,且随黄芪注射液剂量增加,改善程度依次增加,其中高黄芪注射液组与阳性对照组组织病理呈现出相似特征;模型组Schmidt评分、AMS、Hofouaer评分PaCO_(2)均高于对照组,PaO_(2)均低于对照组(P<0.05);低、中、高黄芪注射液组Schmidt评分、AMS、Hofouaer评分PaCO_(2)依次降低,PaO_(2)依次升高(P<0.05);模型组TNF-α、IL-6高于对照组(P<0.05);低、中、高黄芪注射液组TNF-α、IL-6依次降低(P<0.05);模型组mTOR、p70S6K、p-p70S6K、p-mTOR、4EBP1高于对照组(P<0.05);低、中、高黄芪注射液组mTOR、p70S6K、p-p70S6K、p-mTOR、4EBP1依次降低(P<0.05).结论黄芪注射液可呈剂量依赖性改善AHNP血气指标、炎症反应、胰腺与肺组织病理状态,其机制可能是通过调控mTOR/p70S6K信号通路实现的,抑制mTOR/p70S6K信号通路可能为AHNP肺损伤的治疗提供了一个新思路.展开更多
The Eastern Kunlun Orogenic Belt(EKOB)has a complex geological structure and diverse magmatic activities,which are closely related to the Qaidam Basin and the Tethys tectonic evolution.There are at least 3 stages mafi...The Eastern Kunlun Orogenic Belt(EKOB)has a complex geological structure and diverse magmatic activities,which are closely related to the Qaidam Basin and the Tethys tectonic evolution.There are at least 3 stages mafic-ultramafic rocks occurred in the Early Paleozoic in EKOB.The first stage is the Later-Silurian to Early Devonian,represented by the giant Xiarihamu super large magmatic Cu-Ni deposit,containing about 1.18 million metric tons(Mt)of nickel with average grades of 0.65%Ni,and its age of ore-forming pyroxene peridotite is 411 Ma;The second stage is the Early Carboniferous,represented by the large Shitoukengde magmatic Cu-Ni sulfide deposit,and its ore-forming age of the olivine websterite is 334 Ma;The third stage of mafic-ultramafic rocks occurred mainly during the Middle-Late Triassic,represented by Xiaojianshan,Lalinggaoli,and Kaimuqi complexes,and no economical ore bodies have been found in this period.The authors summarized the difference between the ore-bearing and the nonmineralized mafic-ultramafic rocks in the EKOB.The olivine of the ore-bearing complexes contains higher MgO and SiO2 content but lower FeO and CaO contents,and the clinopyroxene of ore-bearing complexes contains lower FeO and CaO contents.Crustal sulfur contamination is key to the formation of the giant Xiarihamu Ni deposit,and crustal sulfur contamination degree of the giant magmatic Ni deposit is higher than that of large Ni deposit.The above indicators could guide the exploration and evaluation of similar deposits in the EKOB.展开更多
文摘背景急性出血坏死型胰腺炎是临床常见的消化系统急症,肺损伤是其常见并发症和致死原因.黄芪注射液具有调节内分泌、增强免疫力、抑制病毒、抗炎、抗氧化应激等作用,但目前尚未见关于黄芪注射液在重症急性胰腺炎肺损伤中的相关研究.目的分析黄芪注射液通过调控雷帕霉素靶蛋白(mammalian target of rapamycin,mTOR)/核糖体70S小亚基S6蛋白激酶(ribosome 70S small subunit S6 protein kinase,p70S6K)信号通路对急性出血坏死型胰腺炎(acute hemorrhagic necrotic pancreatitis,AHNP)肺损伤炎症反应的影响.方法48只成年SD大鼠简单随机化分为对照组、模型组、低、中、高黄芪注射液组、阳性对照组,各8只,记录各组胰腺与肺组织组织病理学变化,比较各组Schmidt评分、血清淀粉酶(amylase,AMS)Hofouaer评分、动脉血二氧化碳分压(PaCO_(2))、动脉血氧分压(PaO_(2))、炎症因子[肿瘤坏死因子-α(tumor necrosis factor-alpha,TNF-α)、白介素-6(interleukin-6,IL-6)]、mTOR/p70S6K信号通路蛋白[mTOR、p70S6K、p-p70S6K、p-mTOR、真核启动因子4E结合蛋白1(p-4E binding protein 1,4EBP1)]水平.结果对照组胰腺、肺组织无异常变化;模型组胰腺实质呈片状坏死、充血水肿,胰腺腺泡细胞与间质细胞大量破坏,小叶结构紊乱,大量炎症细胞浸润与红细胞溢出,肺泡结构排卵紊乱、间质水肿、炎性细胞浸润、肺泡间隔增宽、灶性或片状肺不张、出血;低、中、高黄芪注射液组胰腺与模型组相比,呈现出不同程度改善,且随黄芪注射液剂量增加,改善程度依次增加,其中高黄芪注射液组与阳性对照组组织病理呈现出相似特征;模型组Schmidt评分、AMS、Hofouaer评分PaCO_(2)均高于对照组,PaO_(2)均低于对照组(P<0.05);低、中、高黄芪注射液组Schmidt评分、AMS、Hofouaer评分PaCO_(2)依次降低,PaO_(2)依次升高(P<0.05);模型组TNF-α、IL-6高于对照组(P<0.05);低、中、高黄芪注射液组TNF-α、IL-6依次降低(P<0.05);模型组mTOR、p70S6K、p-p70S6K、p-mTOR、4EBP1高于对照组(P<0.05);低、中、高黄芪注射液组mTOR、p70S6K、p-p70S6K、p-mTOR、4EBP1依次降低(P<0.05).结论黄芪注射液可呈剂量依赖性改善AHNP血气指标、炎症反应、胰腺与肺组织病理状态,其机制可能是通过调控mTOR/p70S6K信号通路实现的,抑制mTOR/p70S6K信号通路可能为AHNP肺损伤的治疗提供了一个新思路.
基金This study was financially supported by the Special Fund for Land and Resources Scientific Research of Public Interest(201511020)the Natural Science Foundation of Shaanxi Province(2017JM4002)Natural Science Foundation of China(41873053).
文摘The Eastern Kunlun Orogenic Belt(EKOB)has a complex geological structure and diverse magmatic activities,which are closely related to the Qaidam Basin and the Tethys tectonic evolution.There are at least 3 stages mafic-ultramafic rocks occurred in the Early Paleozoic in EKOB.The first stage is the Later-Silurian to Early Devonian,represented by the giant Xiarihamu super large magmatic Cu-Ni deposit,containing about 1.18 million metric tons(Mt)of nickel with average grades of 0.65%Ni,and its age of ore-forming pyroxene peridotite is 411 Ma;The second stage is the Early Carboniferous,represented by the large Shitoukengde magmatic Cu-Ni sulfide deposit,and its ore-forming age of the olivine websterite is 334 Ma;The third stage of mafic-ultramafic rocks occurred mainly during the Middle-Late Triassic,represented by Xiaojianshan,Lalinggaoli,and Kaimuqi complexes,and no economical ore bodies have been found in this period.The authors summarized the difference between the ore-bearing and the nonmineralized mafic-ultramafic rocks in the EKOB.The olivine of the ore-bearing complexes contains higher MgO and SiO2 content but lower FeO and CaO contents,and the clinopyroxene of ore-bearing complexes contains lower FeO and CaO contents.Crustal sulfur contamination is key to the formation of the giant Xiarihamu Ni deposit,and crustal sulfur contamination degree of the giant magmatic Ni deposit is higher than that of large Ni deposit.The above indicators could guide the exploration and evaluation of similar deposits in the EKOB.
