75 MeV/u oxygen ion (^16O+8) beams supplied by HIRFL were used to irradiate 14615 of spring wheat dry seeds of stable multi-generation. Range of the ions in the seeds is about 11 mm. They can enter the seed from its e...75 MeV/u oxygen ion (^16O+8) beams supplied by HIRFL were used to irradiate 14615 of spring wheat dry seeds of stable multi-generation. Range of the ions in the seeds is about 11 mm. They can enter the seed from its embryo, come out from its top, and penetrate through an entire grain of wheat, length of which is about 6~7 mm. The seeds were vertically inserted in a sample plate of 4 cm in diameter, which can contain about 100 grain of the seeds. During irradiation, the embryo of the seeds faced to ion beams and was irradiated at normal temperature and atmosphere. Irradiation dose was arranged in 1×10^7, 5×10^7, 1×10^8, 2×10^8 and 5×10^8 ions/cm^2 with dose rate of about 3 Gy/min. A part of the irradiated seeds was employed for cytology examination in laboratory, the others were used for breeding in the fields.展开更多
文摘75 MeV/u oxygen ion (^16O+8) beams supplied by HIRFL were used to irradiate 14615 of spring wheat dry seeds of stable multi-generation. Range of the ions in the seeds is about 11 mm. They can enter the seed from its embryo, come out from its top, and penetrate through an entire grain of wheat, length of which is about 6~7 mm. The seeds were vertically inserted in a sample plate of 4 cm in diameter, which can contain about 100 grain of the seeds. During irradiation, the embryo of the seeds faced to ion beams and was irradiated at normal temperature and atmosphere. Irradiation dose was arranged in 1×10^7, 5×10^7, 1×10^8, 2×10^8 and 5×10^8 ions/cm^2 with dose rate of about 3 Gy/min. A part of the irradiated seeds was employed for cytology examination in laboratory, the others were used for breeding in the fields.