The large yellow croaker(Larimichthys crocea)is an important maricultured fish species in southeast China.Body shape is an important economic trait for this species,because consumers prefer to purchase fish that have ...The large yellow croaker(Larimichthys crocea)is an important maricultured fish species in southeast China.Body shape is an important economic trait for this species,because consumers prefer to purchase fish that have a slender shape.Furthermore,investigating the genetic basis of this trait may be useful for understanding the evolution of fish body shape in general.This study randomly selected 500 large yellow croakers to perform genome-wide association study of this trait.We used Genotyping-By-Sequencing technology combined with a genome-wide prediction model(BayesC)to identify and test QTLs.We also compared the association results using BayesC and single-marker analysis,and found that BayesC outperformed single-marker analysis in detecting significant SNPs explaining a proportion of the total genetic variance in this experiment.Using 124,419 SNP markers,10 candidate genes,correspond to 4 QTL regions located around 3.5,1.8,23.9 and 10.8 Mb on chromosomes 2,4,8 and 22 respectively,were suggested to be relevant to the trait.All of these genes may directly or indirectly participate in bone development.These genes may provide a valuable reference for marker-assisted selective breeding and investigating the genetic basis of the evolution of fish body shape.展开更多
基金Kun Ye,Qingkai Chen,Junwei Chen,Yang Liu and other colleagues in the laboratory participated in fish sampling and traits measurement.The work was supported by China Agriculture Research System(CARS-47-G04)Key projects of the Xiamen Southern Ocean Research Centre(14GZY70NF34)the National Natural Science Foundation of China(U1705231).
文摘The large yellow croaker(Larimichthys crocea)is an important maricultured fish species in southeast China.Body shape is an important economic trait for this species,because consumers prefer to purchase fish that have a slender shape.Furthermore,investigating the genetic basis of this trait may be useful for understanding the evolution of fish body shape in general.This study randomly selected 500 large yellow croakers to perform genome-wide association study of this trait.We used Genotyping-By-Sequencing technology combined with a genome-wide prediction model(BayesC)to identify and test QTLs.We also compared the association results using BayesC and single-marker analysis,and found that BayesC outperformed single-marker analysis in detecting significant SNPs explaining a proportion of the total genetic variance in this experiment.Using 124,419 SNP markers,10 candidate genes,correspond to 4 QTL regions located around 3.5,1.8,23.9 and 10.8 Mb on chromosomes 2,4,8 and 22 respectively,were suggested to be relevant to the trait.All of these genes may directly or indirectly participate in bone development.These genes may provide a valuable reference for marker-assisted selective breeding and investigating the genetic basis of the evolution of fish body shape.
