Powdery mildew(PM),caused by Blumeria graminis f.sp.tritici(Bgt),is one of the destructive wheat diseases worldwide.Wild emmer wheat(Triticum turgidum ssp.dicoccoides,WEW),a tetraploid progenitor of common wheat,is a ...Powdery mildew(PM),caused by Blumeria graminis f.sp.tritici(Bgt),is one of the destructive wheat diseases worldwide.Wild emmer wheat(Triticum turgidum ssp.dicoccoides,WEW),a tetraploid progenitor of common wheat,is a valuable genetic resource for wheat disease resistance breeding programs.We developed three hexaploid pre-breeding lines with PM resistance genes derived from three WEW accessions.These resistant pre-breeding lines were crossed with susceptible common wheat accessions.Segregations in the F2populations were 3 resistant:1 susceptible,suggesting a single dominant allele in each resistant parent.Mapping of the resistance gene in each line indicated a single locus on the long arm of chromosome 7A,at the approximate location of previously cloned Pm60 from T.urartu.Sanger sequencing revealed three different Pm60 haplotypes(Hap 3,Hap 5,and Hap 6).Co-segregating diagnostic markers were developed for identification and selection of each haplotype.The resistance function of each haplotype was verified by the virus-induced gene silencing(VIGS).Common wheat lines carrying each of these Pm60 haplotypes were resistant to most Bgt isolates and differences in the response arrays suggested allelic variation in response.展开更多
As a cool season crop, wheat(Triticum aestivum L.) has an optimal daytime growing temperature of 15 ℃ during the reproductive stage. With global climate change, heat stress is becoming an increasingly severe constrai...As a cool season crop, wheat(Triticum aestivum L.) has an optimal daytime growing temperature of 15 ℃ during the reproductive stage. With global climate change, heat stress is becoming an increasingly severe constraint on wheat production. In this review, we summarize recent progress in understanding the molecular mechanisms of heat tolerance in wheat. We firstly describe the impact of heat tolerance on morphology and physiology and its potential effect on agronomic traits. We then review recent discoveries in determining the genetic and molecular factors affecting heat tolerance, including the effects of phytohormone signaling and epigenetic regulation. Finally, we discuss integrative strategies to improve heat tolerance by utilization of existing germplasm including modern cultivars, landraces and related species.展开更多
Hexaploid triticale(×Triticosecale,AABBRR)is an important forage crop and a promising energy plant.Transferring D-genome chromosomes or segments from common wheat(Triticum aestivum)into hexaploid triticale is att...Hexaploid triticale(×Triticosecale,AABBRR)is an important forage crop and a promising energy plant.Transferring D-genome chromosomes or segments from common wheat(Triticum aestivum)into hexaploid triticale is attractive in improving its economically important traits.Here,a hexaploid triticale 6D(6A)substitution line Lin 456 derived from the cross between the octoploid triticale line H400 and the hexaploid wheat Lin 56 was identified and analyzed by genomic in situ hybridization(GISH),fluorescence in situ hybridization(FISH),and molecular markers.The GISH analysis showed that Lin 456 is a hexaploid triticalewith 14 rye(Secale cereale)chromosomes and 28 wheat chromosomes,whereas non-denaturing fluorescence in situ hybridization(ND-FISH)and molecular marker analysis revealed that it is a 6D(6A)substitution line.In contrast to previous studies,the signal of Oligo-pSc119.2 was observed at the distal end of 6DL in Lin 456.The wheat chromosome 6D was associatedwith increased grain weight and decreased spikelet number using the genotypic data combined with the phenotypes of the F2 population in the three environments.The thousand-grain weight and grain width in the substitution individuals were significantly higher than those in the non-substitution individuals in the F2 population across the three environments.We propose that the hexaploid triticale 6D(6A)substitution line Lin 456 can be a valuable and promising donor stock for genetic improvement during triticale breeding.展开更多
Root hairs are fast growing,ephemeral tubular extensions of the root epidermis that aid nutrient and water uptake.The aim of the present study was to identify QTL for root hair length(RHL)using 227 F8 recombinant inbr...Root hairs are fast growing,ephemeral tubular extensions of the root epidermis that aid nutrient and water uptake.The aim of the present study was to identify QTL for root hair length(RHL)using 227 F8 recombinant inbred lines(RILs)derived from a cross of Zhou 8425 B(Z8425 B)and Chinese Spring(CS),and to develop convenient molecular markers for markerassisted breeding in wheat.Analysis of variance of root hair length showed significant differences(P<0.01)among RILs.The genetic map for QTL analysis consisted of 3389 unique SNP markers.Using composite interval mapping,four major QTL(LOD>2.5)for RHL were identified on chromosomes 1 B(2),2 D and 6 D and four putative QTL(2≤LOD≤2.5)were detected on chromosomes 1 A,3 A,6 B,and 7 B,explaining 3.32%–6.52%of the phenotypic variance.The positive alleles for increased RHL of QTL on chromosomes 2 D,6 B and 6 D(QRhl.cau-2 D,q Rhl.cau-6 B,and QRhl.cau-6 D)were contributed by Z8425 B,and CS contributed positive QTL alleles on chromosomes 1 A(q Rhl.cau-1 A),1 B(QRhl.cau-1 B.1 and QRhl.cau-1 B.2),3 A(q Rhl.cau-3 A)and 7 B(q Rhl.cau-7 B).STARP markers were developed for QRhl.cau-1 B.1,QRhl.cau-2 D,QRhl.cau-6 D,and q Rhl.cau-7 B.Haplotype and association analysis indicated that the positive allele of QRhl.cau-6 D had been strongly selected in Chinese wheat breeding programs.Collectively,the identified QTL for root hair length are likely to be useful for marker-assisted selection.展开更多
Gene regulation is central to all aspects of organism growth,and understanding it using large-scale functional datasets can provide a whole view of biological processes controlling complex phenotypic traits in crops.H...Gene regulation is central to all aspects of organism growth,and understanding it using large-scale functional datasets can provide a whole view of biological processes controlling complex phenotypic traits in crops.However,the connection between massive functional datasets and trait-associated gene discovery for crop improvement is still lacking.In this study,we constructed a wheat integrative gene regulatory network(wGRN)by combining an updated genome annotation and diverse complementary functional datasets,including gene expression,sequence motif,transcription factor(TF)binding,chromatin accessibility,and evolutionarily conserved regulation.wGRN contains 7.2 million genome-wide interactions covering 5947 TFs and 127439 target genes,which were further verified using known regulatory relationships,condition-specific expression,gene functional information,and experiments.We used wGRN to assign genome-wide genes to 3891 specific biological pathways and accurately prioritize candidate genes associated with complex phenotypic traits in genome-wide association studies.In addition,wGRN was used to enhance the interpretation of a spike temporal transcriptome dataset to construct high-resolution networks.We further unveiled novel regulators that enhance the power of spike phenotypic trait prediction using machine learning and contribute to the spike phenotypic differences among modern wheat accessions.Finally,we developed an interactive webserver,wGRN(http://wheat.cau.edu.cn/wGRN),for the community to explore gene regulation and discover trait-associated genes.Collectively,this community resource establishes the foundation for using large-scale functional datasets to guide trait-associated gene discovery for crop improvement.展开更多
Awns are important morphological markers for wheat and exert a strong physiological effect on wheat yield.The awn elongation suppressor B1 has recently been cloned through association and linkage analysis in wheat.How...Awns are important morphological markers for wheat and exert a strong physiological effect on wheat yield.The awn elongation suppressor B1 has recently been cloned through association and linkage analysis in wheat.However,the mechanism of awn inhibition centered around B1 remains to be clarified.Here,we identified an allelic variant in the coding region of B1 through analysis of re-sequencing data;this variant causes an amino acid substitution and premature termination,resulting in a long-awn phenotype.Transcriptome analysis indicated that B1 inhibited awn elongation by impeding cytokinin-and auxinpromoted cell division.Moreover,B1 directly repressed the expression of TaRAE2 and TaLks2,whose orthologs have been reported to promote awn development in rice or barley.More importantly,we found that TaTCP4 and TaTCP10 synergistically inhibited the expression of B1,and a G-to-A mutation in the B1 promoter attenuated its inhibition by TaTCP4/10.Taken together,our results reveal novel mechanisms of awn development and provide genetic resources for trait improvement in wheat.展开更多
Interploidy hybridization between hexaploid and tetraploid genotypes occurred repeatedly during genomic introgression events throughout wheat evolution,and is commonly employed in wheat breeding programs.Hexaploid whe...Interploidy hybridization between hexaploid and tetraploid genotypes occurred repeatedly during genomic introgression events throughout wheat evolution,and is commonly employed in wheat breeding programs.Hexaploid wheat usually serves as maternal parent because the reciprocal cross generates progeny with severe defects and poor seed germination,but the underlying mechanism is poorly understood.Here,we performed detailed analysis of phenotypic variation in endosperm between two interploidy reciprocal crosses arising from tetraploid(Triticum durum,AABB)and hexaploid wheat(Triticum aestivum,AABBDD).In the paternal‐versus the maternal‐excess cross,the timing of endosperm cellularization was delayed and starch granule accumulation in the endosperm was repressed,causing reduced germination percentage.The expression profiles of genes involved in nutrient metabolism differed strongly between these endosperm types.Furthermore,expression patterns of parental alleles were dramatically disturbed in interploidy versus intraploidy crosses,leading to increased number of imprinted genes.The endosperm‐specific TaLFL2 showed a paternally imprinted expression pattern in interploidy crosses partially due to allele‐specific DNA methylation.Paternal TaLFL2 binds to and represses a nutrient accumulation regulator TaNAC019,leading to reduced storage protein and starch accumulation during endosperm development in paternal‐excess cross,as confirmed by interploidy crosses between tetraploid wild‐type and clustered regularly interspaced palindromic repeats(CRISPR)–CRISPR‐associated protein 9 generated hexaploid mutants.These findings reveal a contribution of genomic imprinting to paternal‐excess interploidy hybridization barriers during wheat evolution history and explains why experienced breeders preferentially exploit maternal‐excess interploidy crosses in wheat breeding programs.展开更多
Bread wheat(Triticum aestivum L.)is one of the most commonly consumed staples worldwide,with widespread uses in foods such as breads,noodles,cakes,and cookies(Veraverbeke and Delcour,2002).The specific cooking propert...Bread wheat(Triticum aestivum L.)is one of the most commonly consumed staples worldwide,with widespread uses in foods such as breads,noodles,cakes,and cookies(Veraverbeke and Delcour,2002).The specific cooking properties of wheat products are mainly conferred by the gluten proteins contained in wheat grains(Delcour et al.,2012).High molecular weight glutenin subunits(HMW-GSs)are important constituents of wheat gluten proteins,largely determining gluten elasticity and processing quality(Branlard and Dardevet,1985;Payne et al.,1988).展开更多
Common wheat is a staple food for 35%of the global population,therefore increasing wheat yield in an ever-changing environment is essential for food security in the present day(Peng et al.,2011).Root system is respons...Common wheat is a staple food for 35%of the global population,therefore increasing wheat yield in an ever-changing environment is essential for food security in the present day(Peng et al.,2011).Root system is responsible for water and nutrient acquisition,thus crucial for competitive fitness and crop yield in challenging environments(Karlova et al.,2021;Liu et al.,2022).Over the past decades,extensive research has focused on identifying genes accountable for root growth and development in plants(Rogers and Benfey,2015).However,only a limited number of genes have been cloned in wheat(Li et al.,2021).展开更多
Bread wheat(Triticum aestivum L.)is a major crop that feeds 40%of the world’s population.Over the past several decades,advances in genomics have led to tremendous achievements in understanding the origin and domestic...Bread wheat(Triticum aestivum L.)is a major crop that feeds 40%of the world’s population.Over the past several decades,advances in genomics have led to tremendous achievements in understanding the origin and domestication of wheat,and the genetic basis of agronomically important traits,which promote the breeding of elite varieties.In this review,we focus on progress that has been made in genomic research and genetic improvement of traits such as grain yield,end-use traits,flowering regulation,nutrient use efficiency,and biotic and abiotic stress responses,and various breeding strategies that contributed mainly by Chinese scientists.Functional genomic research in wheat is entering a new era with the availability of multiple reference wheat genome assemblies and the development of cutting-edge technologies such as precise genome editing tools,highthroughput phenotyping platforms,sequencing-based cloning strategies,high-efficiency genetic transformation systems,and speed-breeding facilities.These insights will further extend our understanding of the molecular mechanisms and regulatory networks underlying agronomic traits and facilitate the breeding process,ultimately contributing to more sustainable agriculture in China and throughout the world.展开更多
Dear Editor,Introduction of gibberellin(GA)-insensitive Reduced height(Rht)genes,Rht-B1b and Rht-D1b,has resulted in the“Green Revolution”in modern wheat cultivars(Triticum aestivum)that has skyrocketed wheat grain ...Dear Editor,Introduction of gibberellin(GA)-insensitive Reduced height(Rht)genes,Rht-B1b and Rht-D1b,has resulted in the“Green Revolution”in modern wheat cultivars(Triticum aestivum)that has skyrocketed wheat grain yields worldwide since the 1960s(Peng et al.,1999;Velde et al.,2021).However,Rht-B1b/D1b also reduce coleoptiles,which is undesired in dryland regions where deep planting is essential for seedling establishment(Rebetzke et al.,1999,Rebetzke et al.,2001;Ellis et al.,2004).展开更多
Polish wheat (Triticum polonicum) is a unique tetraploid wheat species characterized by an elongated outer glume. The genetic control of the long-glume trait by a single semi-dominant locus, P1 (from Polish wheat), wa...Polish wheat (Triticum polonicum) is a unique tetraploid wheat species characterized by an elongated outer glume. The genetic control of the long-glume trait by a single semi-dominant locus, P1 (from Polish wheat), was established more than 100 years ago, but the underlying causal gene and molecular nature remain elusive. Here, we report the isolation of VRT-A2, encoding an SVP-clade MADS-box transcription factor, as the P1 candidate gene. Genetic evidence suggests that in T. polonicum, a naturally occurring sequence rearrangement in the intron-1 region of VRT-A2 leads to ectopic expression of VRT-A2 in floral organs where the long-glume phenotype appears. Interestingly, we found that the intron-1 region is a key ON/OFF molecular switch for VRT-A2 expression, not only because it recruits transcriptional repressors, but also because it confers intron-mediated transcriptional enhancement. Genotypic analyses using wheat accessions indicated that the P1 locus is likely derived from a single natural mutation in tetraploid wheat, which was subsequently inherited by hexaploid T. petropavlovskyi. Taken together, our findings highlight the promoter-proximal intron variation as a molecular basis for phenotypic differentiation, and thus species formation in Triticum plants.展开更多
Wheat production requires at least-2.4%increase per year rate by 2050 globally to meet food demands.However,heat stress results in serious yield loss of wheat worldwide.Correspondingly,wheat has evolved genetic basis ...Wheat production requires at least-2.4%increase per year rate by 2050 globally to meet food demands.However,heat stress results in serious yield loss of wheat worldwide.Correspondingly,wheat has evolved genetic basis and molecular mechanisms to protect themselves from heat-induced damage.Thus,it is very urgent to understand the underlying genetic basis and molecular mechanisms responsive to elevated temperatures to provide important strategies for heat-tolerant varieties breeding.In this review,we focused on the impact of heat stress on morphology variation at adult stage in wheat breeding programs.We also summarize the recent studies of genetic and molecular factors regulating heat tolerance,including identification of heat stress tolerance related QTLs/genes,and the regulation pathway in response to heat stress.In addition,we discuss the potential ways to improve heat tolerance by developing new technologies such as genome editing.This review of wheat responses to heat stress may shed light on the understanding heat-responsive mechanisms,although the regu-latory network of heat tolerance is still ambiguous in wheat.展开更多
基金supported by grants from the National Key Research and Development Program of China(2023YFF1000404,2022YFF10001501)the National Natural Science Foundation of China(32171971)。
文摘Powdery mildew(PM),caused by Blumeria graminis f.sp.tritici(Bgt),is one of the destructive wheat diseases worldwide.Wild emmer wheat(Triticum turgidum ssp.dicoccoides,WEW),a tetraploid progenitor of common wheat,is a valuable genetic resource for wheat disease resistance breeding programs.We developed three hexaploid pre-breeding lines with PM resistance genes derived from three WEW accessions.These resistant pre-breeding lines were crossed with susceptible common wheat accessions.Segregations in the F2populations were 3 resistant:1 susceptible,suggesting a single dominant allele in each resistant parent.Mapping of the resistance gene in each line indicated a single locus on the long arm of chromosome 7A,at the approximate location of previously cloned Pm60 from T.urartu.Sanger sequencing revealed three different Pm60 haplotypes(Hap 3,Hap 5,and Hap 6).Co-segregating diagnostic markers were developed for identification and selection of each haplotype.The resistance function of each haplotype was verified by the virus-induced gene silencing(VIGS).Common wheat lines carrying each of these Pm60 haplotypes were resistant to most Bgt isolates and differences in the response arrays suggested allelic variation in response.
基金supported in part by the National Key Research and Development Program of China (2016YFD0101802, 2016YFD0100600)the National Natural Science Foundation of China (31561143013)
文摘As a cool season crop, wheat(Triticum aestivum L.) has an optimal daytime growing temperature of 15 ℃ during the reproductive stage. With global climate change, heat stress is becoming an increasingly severe constraint on wheat production. In this review, we summarize recent progress in understanding the molecular mechanisms of heat tolerance in wheat. We firstly describe the impact of heat tolerance on morphology and physiology and its potential effect on agronomic traits. We then review recent discoveries in determining the genetic and molecular factors affecting heat tolerance, including the effects of phytohormone signaling and epigenetic regulation. Finally, we discuss integrative strategies to improve heat tolerance by utilization of existing germplasm including modern cultivars, landraces and related species.
基金supported by the National Key Research and Development Program of China (2017YFD0101004)the National Natural Science Foundation of China (91435204)the Science and Technology Independent Innovation Ability Upgrading Project of Shanxi Academy of Agricultural Sciences (2017ZZCX-23)
文摘Hexaploid triticale(×Triticosecale,AABBRR)is an important forage crop and a promising energy plant.Transferring D-genome chromosomes or segments from common wheat(Triticum aestivum)into hexaploid triticale is attractive in improving its economically important traits.Here,a hexaploid triticale 6D(6A)substitution line Lin 456 derived from the cross between the octoploid triticale line H400 and the hexaploid wheat Lin 56 was identified and analyzed by genomic in situ hybridization(GISH),fluorescence in situ hybridization(FISH),and molecular markers.The GISH analysis showed that Lin 456 is a hexaploid triticalewith 14 rye(Secale cereale)chromosomes and 28 wheat chromosomes,whereas non-denaturing fluorescence in situ hybridization(ND-FISH)and molecular marker analysis revealed that it is a 6D(6A)substitution line.In contrast to previous studies,the signal of Oligo-pSc119.2 was observed at the distal end of 6DL in Lin 456.The wheat chromosome 6D was associatedwith increased grain weight and decreased spikelet number using the genotypic data combined with the phenotypes of the F2 population in the three environments.The thousand-grain weight and grain width in the substitution individuals were significantly higher than those in the non-substitution individuals in the F2 population across the three environments.We propose that the hexaploid triticale 6D(6A)substitution line Lin 456 can be a valuable and promising donor stock for genetic improvement during triticale breeding.
基金supported by the National Key Research and Development Program of China(2017YFD0101004)the National Natural Science Foundation of China(31991214)。
文摘Root hairs are fast growing,ephemeral tubular extensions of the root epidermis that aid nutrient and water uptake.The aim of the present study was to identify QTL for root hair length(RHL)using 227 F8 recombinant inbred lines(RILs)derived from a cross of Zhou 8425 B(Z8425 B)and Chinese Spring(CS),and to develop convenient molecular markers for markerassisted breeding in wheat.Analysis of variance of root hair length showed significant differences(P<0.01)among RILs.The genetic map for QTL analysis consisted of 3389 unique SNP markers.Using composite interval mapping,four major QTL(LOD>2.5)for RHL were identified on chromosomes 1 B(2),2 D and 6 D and four putative QTL(2≤LOD≤2.5)were detected on chromosomes 1 A,3 A,6 B,and 7 B,explaining 3.32%–6.52%of the phenotypic variance.The positive alleles for increased RHL of QTL on chromosomes 2 D,6 B and 6 D(QRhl.cau-2 D,q Rhl.cau-6 B,and QRhl.cau-6 D)were contributed by Z8425 B,and CS contributed positive QTL alleles on chromosomes 1 A(q Rhl.cau-1 A),1 B(QRhl.cau-1 B.1 and QRhl.cau-1 B.2),3 A(q Rhl.cau-3 A)and 7 B(q Rhl.cau-7 B).STARP markers were developed for QRhl.cau-1 B.1,QRhl.cau-2 D,QRhl.cau-6 D,and q Rhl.cau-7 B.Haplotype and association analysis indicated that the positive allele of QRhl.cau-6 D had been strongly selected in Chinese wheat breeding programs.Collectively,the identified QTL for root hair length are likely to be useful for marker-assisted selection.
基金supported by the National Key Research and Development Program of China(2021YFD1200104)the National Natural Science Foundation of China(31991210)+2 种基金the Strategic International Science and Technology Innovation Collaboration Project(2020YFE0202300)the 2115 Talent Development Program of China Agricultural Universitysupported by High-performance Computing Platform of China Agricultural University.
文摘Gene regulation is central to all aspects of organism growth,and understanding it using large-scale functional datasets can provide a whole view of biological processes controlling complex phenotypic traits in crops.However,the connection between massive functional datasets and trait-associated gene discovery for crop improvement is still lacking.In this study,we constructed a wheat integrative gene regulatory network(wGRN)by combining an updated genome annotation and diverse complementary functional datasets,including gene expression,sequence motif,transcription factor(TF)binding,chromatin accessibility,and evolutionarily conserved regulation.wGRN contains 7.2 million genome-wide interactions covering 5947 TFs and 127439 target genes,which were further verified using known regulatory relationships,condition-specific expression,gene functional information,and experiments.We used wGRN to assign genome-wide genes to 3891 specific biological pathways and accurately prioritize candidate genes associated with complex phenotypic traits in genome-wide association studies.In addition,wGRN was used to enhance the interpretation of a spike temporal transcriptome dataset to construct high-resolution networks.We further unveiled novel regulators that enhance the power of spike phenotypic trait prediction using machine learning and contribute to the spike phenotypic differences among modern wheat accessions.Finally,we developed an interactive webserver,wGRN(http://wheat.cau.edu.cn/wGRN),for the community to explore gene regulation and discover trait-associated genes.Collectively,this community resource establishes the foundation for using large-scale functional datasets to guide trait-associated gene discovery for crop improvement.
基金supported by the National Key Research and Development Program of China(2022YFF1003401)the National Natural Science Foundation of China(31991210)the National Natural Science Foundation of China(32172069).
文摘Awns are important morphological markers for wheat and exert a strong physiological effect on wheat yield.The awn elongation suppressor B1 has recently been cloned through association and linkage analysis in wheat.However,the mechanism of awn inhibition centered around B1 remains to be clarified.Here,we identified an allelic variant in the coding region of B1 through analysis of re-sequencing data;this variant causes an amino acid substitution and premature termination,resulting in a long-awn phenotype.Transcriptome analysis indicated that B1 inhibited awn elongation by impeding cytokinin-and auxinpromoted cell division.Moreover,B1 directly repressed the expression of TaRAE2 and TaLks2,whose orthologs have been reported to promote awn development in rice or barley.More importantly,we found that TaTCP4 and TaTCP10 synergistically inhibited the expression of B1,and a G-to-A mutation in the B1 promoter attenuated its inhibition by TaTCP4/10.Taken together,our results reveal novel mechanisms of awn development and provide genetic resources for trait improvement in wheat.
基金This work was supported by the National Natural Science of China(31471479)the Chinese Universities Scientific Fund(2017TC035).
文摘Interploidy hybridization between hexaploid and tetraploid genotypes occurred repeatedly during genomic introgression events throughout wheat evolution,and is commonly employed in wheat breeding programs.Hexaploid wheat usually serves as maternal parent because the reciprocal cross generates progeny with severe defects and poor seed germination,but the underlying mechanism is poorly understood.Here,we performed detailed analysis of phenotypic variation in endosperm between two interploidy reciprocal crosses arising from tetraploid(Triticum durum,AABB)and hexaploid wheat(Triticum aestivum,AABBDD).In the paternal‐versus the maternal‐excess cross,the timing of endosperm cellularization was delayed and starch granule accumulation in the endosperm was repressed,causing reduced germination percentage.The expression profiles of genes involved in nutrient metabolism differed strongly between these endosperm types.Furthermore,expression patterns of parental alleles were dramatically disturbed in interploidy versus intraploidy crosses,leading to increased number of imprinted genes.The endosperm‐specific TaLFL2 showed a paternally imprinted expression pattern in interploidy crosses partially due to allele‐specific DNA methylation.Paternal TaLFL2 binds to and represses a nutrient accumulation regulator TaNAC019,leading to reduced storage protein and starch accumulation during endosperm development in paternal‐excess cross,as confirmed by interploidy crosses between tetraploid wild‐type and clustered regularly interspaced palindromic repeats(CRISPR)–CRISPR‐associated protein 9 generated hexaploid mutants.These findings reveal a contribution of genomic imprinting to paternal‐excess interploidy hybridization barriers during wheat evolution history and explains why experienced breeders preferentially exploit maternal‐excess interploidy crosses in wheat breeding programs.
基金supported by the National Natural Science Foundation of China(32125030)Hainan Yazhou Bay Seed Lab(B21HJ0502)China Postdoctoral Science Foundation(2022M713413).
文摘Bread wheat(Triticum aestivum L.)is one of the most commonly consumed staples worldwide,with widespread uses in foods such as breads,noodles,cakes,and cookies(Veraverbeke and Delcour,2002).The specific cooking properties of wheat products are mainly conferred by the gluten proteins contained in wheat grains(Delcour et al.,2012).High molecular weight glutenin subunits(HMW-GSs)are important constituents of wheat gluten proteins,largely determining gluten elasticity and processing quality(Branlard and Dardevet,1985;Payne et al.,1988).
基金supported by funds of the National Natural Science Foundation of China(U22A6009,32201824).
文摘Common wheat is a staple food for 35%of the global population,therefore increasing wheat yield in an ever-changing environment is essential for food security in the present day(Peng et al.,2011).Root system is responsible for water and nutrient acquisition,thus crucial for competitive fitness and crop yield in challenging environments(Karlova et al.,2021;Liu et al.,2022).Over the past decades,extensive research has focused on identifying genes accountable for root growth and development in plants(Rogers and Benfey,2015).However,only a limited number of genes have been cloned in wheat(Li et al.,2021).
基金This work was supported by the National Natural Science Foundation of China(31788103,31970529,32125030,31921005,31961143013,32072660)the Key Research and Development Program of Ministry of Science and Technology of China(2021YFF1000200)the Strategic Priority Research Program of Chinese Academy of Sciences(XDA24010202).
文摘Bread wheat(Triticum aestivum L.)is a major crop that feeds 40%of the world’s population.Over the past several decades,advances in genomics have led to tremendous achievements in understanding the origin and domestication of wheat,and the genetic basis of agronomically important traits,which promote the breeding of elite varieties.In this review,we focus on progress that has been made in genomic research and genetic improvement of traits such as grain yield,end-use traits,flowering regulation,nutrient use efficiency,and biotic and abiotic stress responses,and various breeding strategies that contributed mainly by Chinese scientists.Functional genomic research in wheat is entering a new era with the availability of multiple reference wheat genome assemblies and the development of cutting-edge technologies such as precise genome editing tools,highthroughput phenotyping platforms,sequencing-based cloning strategies,high-efficiency genetic transformation systems,and speed-breeding facilities.These insights will further extend our understanding of the molecular mechanisms and regulatory networks underlying agronomic traits and facilitate the breeding process,ultimately contributing to more sustainable agriculture in China and throughout the world.
基金This work was supported by the grants from the National Natural Science Foundation of China(grants 91935302 and 31991210)Hainan Yazhou Bay Seed Laboratory(B21HJ0111).
文摘Dear Editor,Introduction of gibberellin(GA)-insensitive Reduced height(Rht)genes,Rht-B1b and Rht-D1b,has resulted in the“Green Revolution”in modern wheat cultivars(Triticum aestivum)that has skyrocketed wheat grain yields worldwide since the 1960s(Peng et al.,1999;Velde et al.,2021).However,Rht-B1b/D1b also reduce coleoptiles,which is undesired in dryland regions where deep planting is essential for seedling establishment(Rebetzke et al.,1999,Rebetzke et al.,2001;Ellis et al.,2004).
基金This work was supported by grants from the National Natural Science Foundation of China(32072055,31991210,and 91935304).
文摘Polish wheat (Triticum polonicum) is a unique tetraploid wheat species characterized by an elongated outer glume. The genetic control of the long-glume trait by a single semi-dominant locus, P1 (from Polish wheat), was established more than 100 years ago, but the underlying causal gene and molecular nature remain elusive. Here, we report the isolation of VRT-A2, encoding an SVP-clade MADS-box transcription factor, as the P1 candidate gene. Genetic evidence suggests that in T. polonicum, a naturally occurring sequence rearrangement in the intron-1 region of VRT-A2 leads to ectopic expression of VRT-A2 in floral organs where the long-glume phenotype appears. Interestingly, we found that the intron-1 region is a key ON/OFF molecular switch for VRT-A2 expression, not only because it recruits transcriptional repressors, but also because it confers intron-mediated transcriptional enhancement. Genotypic analyses using wheat accessions indicated that the P1 locus is likely derived from a single natural mutation in tetraploid wheat, which was subsequently inherited by hexaploid T. petropavlovskyi. Taken together, our findings highlight the promoter-proximal intron variation as a molecular basis for phenotypic differentiation, and thus species formation in Triticum plants.
基金Major Program of the National Natural Science Foundation of China(3213000343)。
文摘Wheat production requires at least-2.4%increase per year rate by 2050 globally to meet food demands.However,heat stress results in serious yield loss of wheat worldwide.Correspondingly,wheat has evolved genetic basis and molecular mechanisms to protect themselves from heat-induced damage.Thus,it is very urgent to understand the underlying genetic basis and molecular mechanisms responsive to elevated temperatures to provide important strategies for heat-tolerant varieties breeding.In this review,we focused on the impact of heat stress on morphology variation at adult stage in wheat breeding programs.We also summarize the recent studies of genetic and molecular factors regulating heat tolerance,including identification of heat stress tolerance related QTLs/genes,and the regulation pathway in response to heat stress.In addition,we discuss the potential ways to improve heat tolerance by developing new technologies such as genome editing.This review of wheat responses to heat stress may shed light on the understanding heat-responsive mechanisms,although the regu-latory network of heat tolerance is still ambiguous in wheat.