It is known that eddy current effect has a great influence on magnetic flux leakage testing(MFL).Usually,contacttype encoder wheels are used to measure MFL testing speed to evaluate the effect and further compensate t...It is known that eddy current effect has a great influence on magnetic flux leakage testing(MFL).Usually,contacttype encoder wheels are used to measure MFL testing speed to evaluate the effect and further compensate testing signals.This speed measurement method is complicated,and inevitable abrasion and occasional slippage will reduce the measurement accuracy.In order to solve this problem,based on eddy current effect due to the relative movement,a speed measurement method is proposed,which is contactless and simple.In the high-speed MFL testing,eddy current induced in the specimen will cause an obvious modification to the applied field.This modified field,which is measured by Hall sensor,can be utilized to reflect the moving speed.Firstly,the measurement principle is illustrated based on Faraday’s law.Then,dynamic finite element simulations are conducted to investigate the modified magnetic field distribution.Finally,laboratory experiments are performed to validate the feasibility of the proposed method.The results show that Bmz(r1)and Bmx(r2)have a linear relation with moving speed,which could be used as an alternative measurement parameter.展开更多
This paper summarized the nutritional value,use,botany characteristics,cultivation status and common varieties of passion fruit(Passiflora edulis Sims).It aims to provide a basis for the cultivation and development of...This paper summarized the nutritional value,use,botany characteristics,cultivation status and common varieties of passion fruit(Passiflora edulis Sims).It aims to provide a basis for the cultivation and development of passion fruit.展开更多
The open reading frame （ORF） of hAPOA1 was inserted into the prokaryotic expression vector pGEX-4T-1 to construct the recombinant plasmid pGEX- 4T-I-hAPOA1, which was then transformed into Escherichia coil strain BL...The open reading frame （ORF） of hAPOA1 was inserted into the prokaryotic expression vector pGEX-4T-1 to construct the recombinant plasmid pGEX- 4T-I-hAPOA1, which was then transformed into Escherichia coil strain BL21. The expression of target fusion protein was induced with isopropyl β-D-l-thiogalacto- pyranoside （IPTG）. The purified fusion protein in inclusion bodies was used to immunize New Zealand white rabbits to prepare hAPOA1 antiserum and the antibody titer was detected with indirect enzyme-linked immunosorbent assay （ID-EL1SA）. ID-ELISA and Western Blot proved that rabbit polyclonal antibody with a high titer of 1 ： 40 000 was produced, which may bring considerable economic benefits.展开更多
基金supported in part by the National Natural Science Foundation of China(Grant No.92060114)in part by the Sichuan Science and Technology Program(Nos.2022YFS0524 and 2022YFG0044).
文摘It is known that eddy current effect has a great influence on magnetic flux leakage testing(MFL).Usually,contacttype encoder wheels are used to measure MFL testing speed to evaluate the effect and further compensate testing signals.This speed measurement method is complicated,and inevitable abrasion and occasional slippage will reduce the measurement accuracy.In order to solve this problem,based on eddy current effect due to the relative movement,a speed measurement method is proposed,which is contactless and simple.In the high-speed MFL testing,eddy current induced in the specimen will cause an obvious modification to the applied field.This modified field,which is measured by Hall sensor,can be utilized to reflect the moving speed.Firstly,the measurement principle is illustrated based on Faraday’s law.Then,dynamic finite element simulations are conducted to investigate the modified magnetic field distribution.Finally,laboratory experiments are performed to validate the feasibility of the proposed method.The results show that Bmz(r1)and Bmx(r2)have a linear relation with moving speed,which could be used as an alternative measurement parameter.
文摘This paper summarized the nutritional value,use,botany characteristics,cultivation status and common varieties of passion fruit(Passiflora edulis Sims).It aims to provide a basis for the cultivation and development of passion fruit.
基金Supported by the Agricultural Science Independent Innovation Fund Jiangsu Province[CX(16)1326]
文摘The open reading frame （ORF） of hAPOA1 was inserted into the prokaryotic expression vector pGEX-4T-1 to construct the recombinant plasmid pGEX- 4T-I-hAPOA1, which was then transformed into Escherichia coil strain BL21. The expression of target fusion protein was induced with isopropyl β-D-l-thiogalacto- pyranoside （IPTG）. The purified fusion protein in inclusion bodies was used to immunize New Zealand white rabbits to prepare hAPOA1 antiserum and the antibody titer was detected with indirect enzyme-linked immunosorbent assay （ID-EL1SA）. ID-ELISA and Western Blot proved that rabbit polyclonal antibody with a high titer of 1 ： 40 000 was produced, which may bring considerable economic benefits.
