氮元素过多过少都可能导致植株表型发生明显变化,甚至影响植株的正常生长发育。为了探究西藏青稞对不同氮浓度的响应,本研究采用藏青13作为材料,利用同位素相对标记与绝对定量技术(isobaric tags for relative and absolute quantitatio...氮元素过多过少都可能导致植株表型发生明显变化,甚至影响植株的正常生长发育。为了探究西藏青稞对不同氮浓度的响应,本研究采用藏青13作为材料,利用同位素相对标记与绝对定量技术(isobaric tags for relative and absolute quantitation, Itraq)标记联合液相色谱串联质谱(LC-MS/MS)技术并结合生物信息学和分子生物学技术对适氮、缺氮、低氮和高氮处理样品的蛋白谱进行分析。iTRAQ测序结果表明,在根组织共鉴定得到8589个蛋白质,肽链长度主要分布在6～18个氨基酸之间;在叶组织中鉴定得到4608个蛋白质,肽链长度主要分布在6～16个氨基酸之间。COG分析显示,R类蛋白数量最多,同时鉴定的蛋白涉及次生代谢生物合成、信号转导、防御机制等多个方面。在不同氮素浓度处理下,其中缺氮处理与对照(适氮处理)相比,所筛选到的差异蛋白最多,有1518个蛋白,其中810个上调,708个下调。四个处理共同筛选到的差异蛋白共富集到47个功能项中,包含20个生物过程GO功能项,15个细胞组分GO功能项,12个分子功能GO功能项。展开更多
为进一步了解冷驯化后的青稞苗对低温胁迫的响应机制,本研究采用藏青148作为材料,利用同位素相对标记与绝对定量技术(isobaric tags for relative and absolute quantitation, iTRAQ)标记联合液相色谱串联质谱(LC-MS/MS)技术并结合生物...为进一步了解冷驯化后的青稞苗对低温胁迫的响应机制,本研究采用藏青148作为材料,利用同位素相对标记与绝对定量技术(isobaric tags for relative and absolute quantitation, iTRAQ)标记联合液相色谱串联质谱(LC-MS/MS)技术并结合生物信息学和分子生物学技术对青稞低温诱导的蛋白组差异蛋白谱进行分析。结果表明,在iTRAQ测序分析的常温及冷驯化后低温处理的样品肽段图谱中,共鉴定出5111个蛋白质,肽链长度主要分布在6-24个氨基酸之间。通路分析显示,鉴定到的蛋白质被富集到126个通路。COG分析显示,R类蛋白数量最多,同时鉴定的蛋白涉及能量代谢、信号转导、防御机制等多个方面。对照常温和低温处理18h筛选到的差异蛋白有886个,存在499个上调,387个下调,且在代谢通路、乙醛酸代谢、三羧酸循环、糖酵解四条通路显著富集。展开更多
Data from the Tibet air shower array were used to examine the cosmic ray shadows of the Moon and the Sun at energies around 10Tev.The shadowing effect was clearly observed at the 5.8level for the Moon,while the shadow...Data from the Tibet air shower array were used to examine the cosmic ray shadows of the Moon and the Sun at energies around 10Tev.The shadowing effect was clearly observed at the 5.8level for the Moon,while the shadow of the Sun was found in the direction away from the sun by 0.86°tothe west and 0.43°to the south.The effect of the geomagnetic field has also been observed in the shadow of cosmic rays by the Moon.The observed deflection of the sun’s shadow is briefly discussed in connection with the effect of the magnetic fields between the sun and the Earth.This is the first observation of the effects of such magnetic fields on the cosmic ray shadow.The maximum-likelihood analysis of the Moon data set shows that the angular resolutions of the array for showers with its mode energies 7TeVand 35TeV are 0.87°-0.13°+0.10°and 0.54°-0.08°+0.11°,respectively.展开更多
文摘氮元素过多过少都可能导致植株表型发生明显变化,甚至影响植株的正常生长发育。为了探究西藏青稞对不同氮浓度的响应,本研究采用藏青13作为材料,利用同位素相对标记与绝对定量技术(isobaric tags for relative and absolute quantitation, Itraq)标记联合液相色谱串联质谱(LC-MS/MS)技术并结合生物信息学和分子生物学技术对适氮、缺氮、低氮和高氮处理样品的蛋白谱进行分析。iTRAQ测序结果表明,在根组织共鉴定得到8589个蛋白质,肽链长度主要分布在6～18个氨基酸之间;在叶组织中鉴定得到4608个蛋白质,肽链长度主要分布在6～16个氨基酸之间。COG分析显示,R类蛋白数量最多,同时鉴定的蛋白涉及次生代谢生物合成、信号转导、防御机制等多个方面。在不同氮素浓度处理下,其中缺氮处理与对照(适氮处理)相比,所筛选到的差异蛋白最多,有1518个蛋白,其中810个上调,708个下调。四个处理共同筛选到的差异蛋白共富集到47个功能项中,包含20个生物过程GO功能项,15个细胞组分GO功能项,12个分子功能GO功能项。
文摘为进一步了解冷驯化后的青稞苗对低温胁迫的响应机制,本研究采用藏青148作为材料,利用同位素相对标记与绝对定量技术(isobaric tags for relative and absolute quantitation, iTRAQ)标记联合液相色谱串联质谱(LC-MS/MS)技术并结合生物信息学和分子生物学技术对青稞低温诱导的蛋白组差异蛋白谱进行分析。结果表明,在iTRAQ测序分析的常温及冷驯化后低温处理的样品肽段图谱中,共鉴定出5111个蛋白质,肽链长度主要分布在6-24个氨基酸之间。通路分析显示,鉴定到的蛋白质被富集到126个通路。COG分析显示,R类蛋白数量最多,同时鉴定的蛋白涉及能量代谢、信号转导、防御机制等多个方面。对照常温和低温处理18h筛选到的差异蛋白有886个,存在499个上调,387个下调,且在代谢通路、乙醛酸代谢、三羧酸循环、糖酵解四条通路显著富集。
基金We are grateful to Prof.J.Arafune of ICRR,University of Tokyo and Prof.S.X.Fan of IHEP,Chinese Academy of Science for their support and encouragement.
文摘Data from the Tibet air shower array were used to examine the cosmic ray shadows of the Moon and the Sun at energies around 10Tev.The shadowing effect was clearly observed at the 5.8level for the Moon,while the shadow of the Sun was found in the direction away from the sun by 0.86°tothe west and 0.43°to the south.The effect of the geomagnetic field has also been observed in the shadow of cosmic rays by the Moon.The observed deflection of the sun’s shadow is briefly discussed in connection with the effect of the magnetic fields between the sun and the Earth.This is the first observation of the effects of such magnetic fields on the cosmic ray shadow.The maximum-likelihood analysis of the Moon data set shows that the angular resolutions of the array for showers with its mode energies 7TeVand 35TeV are 0.87°-0.13°+0.10°and 0.54°-0.08°+0.11°,respectively.
