Network pharmacology and molecular docking to explore Polygoni Cuspidati Rhizoma et Radix treatment for acute lung injury

BACKGROUND Polygoni Cuspidati Rhizoma et Radix(PCRR),a well-known traditional Chinese medicine(TCM),inhibits inflammation associated with various human diseases.However,the anti-inflammatory effects of PCRR in acute lung injury(ALI)and the underlying mechanisms of action remain unclear.AIM To determine the ingredients related to PCRR for treatment of ALI using multiple databases to obtain potential targets for fishing.METHODS Recognized and candidate active compounds for PCRR were obtained from Traditional Chinese Medicine Systems Pharmacology,STITCH,and PubMed databases.Target ALI databases were built using the Therapeutic Target,DrugBank,DisGeNET,Online Mendelian Inheritance in Man,and Genetic Association databases.Network pharmacology includes network construction,target prediction,topological feature analysis,and enrichment analysis.Bioinformatics resources from the Database for Annotation,Visualization and Integrated Discovery were utilized for gene ontology biological process and Kyoto Encyclopedia of Genes and Genomes network pathway enrichment analysis,and molecular docking techniques were adopted to verify the combination of major active ingredients and core targets.RESULTS Thirteen bioactive compounds corresponding to the 433 PCRR targets were identified.In addition,128 genes were closely associated with ALI,60 of which overlapped with PCRR targets and were considered therapeutically relevant.Functional enrichment analysis suggested that PCRR exerted its pharmacological effects in ALI by modulating multiple pathways,including the cell cycle,cell apoptosis,drug metabolism,inflammation,and immune modulation.Molecular docking results revealed a strong associative relationship between the active ingredient and core target.CONCLUSION PCRR alleviates ALI symptoms via molecular mechanisms predicted by network pharmacology.This study proposes a strategy to elucidate the mechanisms of TCM at the network pharmacology level.

RNF2在结直肠癌中的表达及其对结直肠癌细胞生物学行为的影响

目的探讨环指蛋白2 (RNF2)在结直肠癌中的表达及其对结直肠癌细胞生物学行为的影响。方法采用免疫组织化学法检测RNF2在结直肠癌组织和癌旁正常组织中的表达,并利用过表达的慢病毒转染HCT116细胞构建过表达RNF2的HCT116细胞系,通过CCK-8法、划痕实验、Transwell实验检测过表达RNF2对结直肠癌细胞增殖、迁移、侵袭等生物学行为的影响。结果结直肠癌组织中RNF2阳性表达率高于癌旁正常组织(P <0.05);不同年龄、组织学类型、分化程度、Dukes分期、淋巴结转移的结直肠癌患者的RNF2表达阳性率比较,差异有统计学意义(P <0.05);HCT116组、HCT116-control组、HCT116-RNF2组HCT116细胞增殖、迁移能力及侵袭能力比较,差异有统计学意义(P <0.05);HCT116-RNF2组细胞增殖速度、迁移能力及侵袭能力均高于HCT116-control组和HCT116组(P <0.05)。结论 RNF2在结直肠癌中呈高表达,且过表达RNF2可促进结直肠癌细胞迁移、增殖、侵袭能力,可为结直肠癌治疗提供新的治疗靶点。

Preparation and Properties of Nano-TiO_2 Powders

This paper reports the preparation of nano-TiO2 (about 10 nm) powder by the method of precipitation. In detail, some breparation conditions were investigated in order to find out how to control the grain size and reduce the agglomeration of powders. Also, the reflex spectra of nano-scale powders with different grain size were studied. It tvas found that the wave length and width of reflex spectra are connected with the grain size of nano-TiO2 powders

An Experimental Study on Fuel Combustion Under External Irradiance

In order to investigate the burning characteristics of a 0#diesel and 3#jet fuel,a small-scale experimental bench mainly composed of a cone calorimeter was arranged.The heat release and burning rates were investigated changing the external irradiance in order to clarify the triadic relationship among these quantities.The effective heat of combustion of 0#diesel and 3#jet fuel were 38.89 MJ/kg and 43.15 MJ/kg,respectively,with the corresponding combustion efficiencies being 96.78%and 99.60%(the effective peak heat of combustion being 1.665 times the mean value for both types of fuel).According to our experimental results,the heat release rate and burning rate of 0#diesel and 3#jet fuel both grow linearly with an increase in the external irradiance.Moreover,the heat release rate and burning rate of 3#jet fuel are greater than the equivalent values for the 0#diesel.The average smoke production rate of 0#diesel grows with an increase in the external irradiance,while for the 3#jet fuel it remains approximately the same.The specific extinction area of these two kinds of fuel shows a subtle decrease with the increase of external irradiance.The time to ignition of 3#jet fuel is smaller than 0#diesel for all the considered external irradiances,which indicates that 3#jet fuel is easier to ignite than the 0#diesel.

Investigation of gate oxide traps effect on NAND flash memory by TCAD simulation

The effects of gate oxide traps on gate leakage current and device performance of metal–oxide–nitride–oxide–silicon(MONOS)-structured NAND flash memory are investigated through Sentaurus TCAD. The trap-assisted tunneling(TAT)model is implemented to simulate the leakage current of MONOS-structured memory cell. In this study, trap position, trap density, and trap energy are systematically analyzed for ascertaining their influences on gate leakage current, program/erase speed, and data retention properties. The results show that the traps in blocking layer significantly enhance the gate leakage current and also facilitates the cell program/erase. Trap density ~10^(18) cm^(-3) and trap energy ~ 1 eV in blocking layer can considerably improve cell program/erase speed without deteriorating data retention. The result conduces to understanding the role of gate oxide traps in cell degradation of MONOS-structured NAND flash memory.

Effects of Feeding Time on the Growth Performance and Variation of RNA/DNA Ratio of the Chinese Soft-shelled Turtle, Pelodiscus sinensis

The present study was to investigate the effects of feeding time on growth of the Chinese soft-shelled turtle. The experiment was carried out with a photoperiod of 12L （light）： 12D （dark） with lights on at 06：00 and off at 18：00. Turtles were maintained at a temperature of 32 - 0.2 ℃ in tanks throughout the length of the experiment. The turtles in group 1 to group 6 were fed respectively at 00：00, 04：00, 08：00, 12：00, 16：00 and 20：00 with 60 turtles each （Initial body weight 88.27 - 0.09 g）. Acrophases of postprandial RNA/DNA ratio in liver in each group was shown between 5h and 7h after feeding. A positive linear correlation could be seen between specific growth rate （SGR） and RNA/ DNA：SGR=t.1586RNA/DNA-0.7097 （r = 0.9328, P = 0.0066）. The results indicated that the values at the acrophases of about 6h after feeding might be used as an instantaneous growth index in the Chinese soft-shelled turtle. The turtles in group 1 grew better than group 3, group 4, group 5, group 6, because they ate more, but they ate more and grew slower than group 2, whose feed conversion rate was also higher. Meanwhile, the SGR and feeding rate of turtles fed at 12：00 were the lowest from the six groups （P 〈 0.05）. Turtles fed in group 1, group 2 and group 6 developed more heavy final body weight （FBW）, higher feeding rate and SGR than the other three groups. This probably suggested that turtles fed in scotophase grew better than that fed at photophase in total.

ATA Model of Health Emergency Science Popularization in China: A Pilot Study

A recent novel coronavirus(COVID-19)outbreak has started since December 2019 in Wuhan,Hubei province,People’s Republic of China and has progressively expanded to various parts of China.Based on those practical problems of emergency science popularization works in the current epidemic situation,this article,from the perspective of accuracy,timeliness and acceptability of emergency science popularization,proposes that the professional science popularization knowledge should be popularized,rumor should be refuted in a timely manner,and accurate science popularization strategies should be taken for different science preference,so to provide countermeasures and guidance for problems in emergency science popularization.

Large contributions of emission reductions and meteorological conditions to the abatement of PM_(2.5)in Beijing during the 24th Winter Olympic Games in 2022

To guarantee the blue skies for the 2022 Winter Olympics held in Beijing and Zhangjiakou from February 4 to 20,Beijing and its surrounding areas adopted a series of emission control measures.This provides an opportunity to determine the impacts of large-scale temporary control measures on the air quality in Beijing during this special period.Here,we applied the WRF-CMAQ model to quantify the contributions of emission reduction measures and meteorological conditions.Results show that meteorological conditions in 2022 decreased PM_(2.5)in Beijing by 6.9 and 11.8μg/m^(3)relative to 2021 under the scenarios with and without emission reductions,respectively.Strict emission reduction measures implemented in Beijing and seven neighboring provinces resulted in an average decrease of 13.0μg/m^(3)(-41.2%)in PM_(2.5)in Beijing.Over the entire period,local emission reductions contributed more to good air quality in Beijing than nonlocal emission reductions.Under the emission reduction scenario,local,controlled regions,other regions,and boundary conditions contributed 47.7%,42.0%,5.3%,and 5.0%to the PM_(2.5)concentrations in Beijing,respectively.The results indicate that during the cleaning period with the air masses from the northwest,the abatements of PM_(2.5)were mainly caused by local emission reductions.However,during the potential pollution period with the air masses from the east-northeast and west-southwest,the abatements of PM_(2.5)were caused by both local and nonlocal emission reductions almost equally.This implies that regional coordinated prevention and control strategies need to be arranged scientifically and rationally when heavy pollution events are forecasted.

后BEPS时代高新技术企业税收优惠政策再审视

2017年,我国高新技术企业税收优惠政策以"无害"结论通过了有害税收竞争论坛的审议。本文客观审视我国高新技术企业税收优惠政策存在的问题,并结合BEPS行动计划提出的关联法以及其他国家专利盒制度实施情况,提出进一步完善建议,包括:进一步强化对高新技术企业资格的监管,体现对自主研发知识产权的政策倾斜,统一研发费用归集口径,加大高新技术企业税收优惠政策力度。

Iron Isotopes and Trace Element Compositions of Magnetite from the Submarine Volcanic-Hosted Iron Deposits in East Tianshan,NW China:New Insights into the Mineralization Processes

The Aqishan-Yamansu metallogenic belt(AYMB)in East Tianshan hosts abundant sub-marine volcanic-hosted iron deposits.Although there is agreement with the magmatic source of the ore-forming materials and the role of hydrothermal replacement in iron ore formation,the mineraliza-tion processes of these iron deposits remain uncertain.Three ore types are identified on the basis of the geological occurrences of minerals and the sequence of mineral in ores.The typeⅠores are characte-rized by magnetite,diopside,amphibole with a few pyrite,and chalcopyrite.The type II ores are mainly composed of magnetite,garnet,chlorite with a few pyrite,while the type III ores are mainly composed of magnetite,quartz,calcite with a few pyrite.In order to constrain the mineralization processes of these ore types,we performed iron isotopes and trace element compositions of magnetite from three typical iron deposits(Yamansu,Duotoushan and Luotuofeng).Trace element and Fe isotope investiga-tions of the three ore types reveal two major groups.The groupⅠconsists of analyses of the typeⅠandⅡores,with both showing a narrow range of positiveδ56Fe values(+0.08‰to+0.22‰for typeⅠores and+0.15‰ to+0.22‰ for typeⅡores)and plotting in the range of the ortho-magmatic field.In contrast,the group 2 is composed merely of the typeⅢores,showing a wider range of negativeδ56Fe values(-0.49‰ to-0.01‰),which is similar to the features of Fe-skarn magnetite.As shown in the binary dia-grams of magnetite trace elements and a fractionation of the Fe isotopes,different ore types were likely produced during gradually changing ore-forming stages from magmatic to hydrothermal.Collectively,the submarine volcanic-hosted iron deposits in the East Tianshan are likely the results of a continuous magmatic-hydrothermal mineralization process.

SIZ1-Mediated SUMOylation of ROS1 Enhances Its Stability and Positively Regulates Active DNA Demethylation in Arabidopsis

The 5-methylcytosine DNA glycosylase/lyase REPRESSOR OF SILENCING 1(ROS1)-mediated active DNA demethylation is critical for shaping the genomic DNA methylation landscape in Arabidopsis.Whether and how the stability of ROS1 may be regulated by post-translational modifications is unknown.Using a methylation-sensitive PCR(CHOP-PCR)-based forward genetic screen forArabidopsis DNA hyper-methyl-ation mutants,we identified the SUMO E3 ligase SIZ1 as a critical regulator of active DNA demethylation.Dysfunction of SIZ1 leads to hyper-methylation at approximately 1000 genomic regions.SIZ1 physically in-teracts with ROS1 and mediates the SUMOylation of ROS1.The SUMOylation of ROS1 is reduced in siz1 mutant plants.Compared with that in wild-type plants,the protein level of ROS1 is significantly decreased,whereas there is an increased level of ROS1 transcripts in siz1 mutant plants.Our results suggest that SIZ1-mediated SUMOylation of ROS1 promotes its stability and positively regulates active DNA demethylation.

RCEP:我国自贸区战略的理论深化、实践突破和工作启示

签署《区域全面经济伙伴关系协定》(RCEP)是我国加入世界贸易组织后的又一重大开放成果,对于促进形成更高水平对外开放格局具有重要战略意义。本文从梳理我国自贸区战略的历史发展脉络出发,分析签署RCEP对我国自贸区战略的理论发展和实践突破,力图为进一步深化研究提供新的视角;同时,立足国内“十四五”规划建设开局起步和美国拜登政府上台后世界经贸格局面临新调整的背景形势,分析新时期实现自贸区提升战略的风险挑战,提出进一步构建面向全球的高标准自贸区网络的政策建议。

Structure and electrical properties of ternary BiFeO_(3)-BaTiO_(3)-PbTiO_(3) high-temperature piezoceramics

In the current work,the bulk ternary(0.85-x)BiFeO3-xBaTiO3-0.15PbTiO3(BF-BTx-PT,x=0.08-0.35)system has been studied as a potential high-temperature piezoceramics.Samples with various content of BT were prepared via solid-state route,and pure perovskite phase was confirmed by X-ray diffraction.The temperature dependence of dielectric constants confirmed the decrease of Curie temperature with increasing BT content.It was found that the morphotropic phase boundary(MPB)composition of BF-BTx-PT ceramics was in the vicinity of x=0.15,which exhibits optimal properties with piezoelectric constant d33 of 60 pC/N,high Curie temperature of 550℃,and low sintering temperature of 920℃.Measurements also showed that the depoling temperature was 300℃,about 150℃higher than that of commercialized PZT ceramics,which indicated good temperature stability.BF-BTx-PT ceramics are promising candidates for high temperature applications.